FastQCFastQC Report
Thu 2 Feb 2017
SRR4063069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090081
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT22940.2104430771658253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16090.14760371018300475No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11150.10228597691364219No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11040.10127687758983049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG756.401751E-821.33330215
CGAACGA801.2653618E-719.99997116
TTTTCGG2400.019.33330329
TTTCGGA2350.019.06380330
CTAGCGG1900.018.52628929
TAACGAA904.366284E-717.77775213
TCTAGCG2000.017.59997628
TTCGGAA2500.017.27997631
GCGTAAC1651.8189894E-1216.48482511
ATCGTTT1750.016.45711929
TAGCGGC2150.016.3720730
AACGAAC1001.3142362E-615.99997714
GTTTTCG2700.015.99997728
CGCGTAA1501.1459633E-1015.99997610
TCGCGTA1501.1459633E-1015.9999769
ACGCTGA2750.015.70906911
CGTTTAT1753.6379788E-1215.54283531
GTCCTAT2800.015.4292571
CGATAAC1052.18461E-615.23807310
ACACGCT2750.015.1272529
Produced by FastQC (version 0.11.5)