##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090081
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172011070736946	32.0	32.0	32.0	32.0	32.0
2	31.227786742453084	32.0	32.0	32.0	32.0	32.0
3	31.347840206369984	32.0	32.0	32.0	32.0	32.0
4	31.446335639278182	32.0	32.0	32.0	32.0	32.0
5	31.36722133492832	32.0	32.0	32.0	32.0	32.0
6	34.87710638016808	36.0	36.0	36.0	36.0	36.0
7	34.91442929470379	36.0	36.0	36.0	36.0	36.0
8	34.83978254826935	36.0	36.0	36.0	32.0	36.0
9	34.973739566142335	36.0	36.0	36.0	36.0	36.0
10	34.799561683948255	36.0	36.0	36.0	32.0	36.0
11	34.987921998457	36.0	36.0	36.0	36.0	36.0
12	34.883948073583525	36.0	36.0	36.0	32.0	36.0
13	34.94617647679392	36.0	36.0	36.0	36.0	36.0
14	34.88208123983447	36.0	36.0	36.0	32.0	36.0
15	34.86022231375467	36.0	36.0	36.0	32.0	36.0
16	34.86802815570586	36.0	36.0	36.0	32.0	36.0
17	34.840604505536746	36.0	36.0	36.0	32.0	36.0
18	34.838956921549865	36.0	36.0	36.0	32.0	36.0
19	34.831185022030475	36.0	36.0	36.0	32.0	36.0
20	34.82064635563779	36.0	36.0	36.0	32.0	36.0
21	34.803280673638014	36.0	36.0	36.0	32.0	36.0
22	34.793123630262336	36.0	36.0	36.0	32.0	36.0
23	34.74053120823132	36.0	36.0	36.0	32.0	36.0
24	34.717361370393576	36.0	36.0	36.0	32.0	36.0
25	34.70261017300549	36.0	36.0	36.0	32.0	36.0
26	34.645391489256305	36.0	36.0	36.0	32.0	36.0
27	34.62639657053008	36.0	36.0	36.0	32.0	36.0
28	34.59436867535532	36.0	36.0	36.0	32.0	36.0
29	34.571568534815306	36.0	36.0	36.0	32.0	36.0
30	34.54358345847694	36.0	36.0	36.0	32.0	36.0
31	34.538280182848794	36.0	36.0	36.0	32.0	36.0
32	34.52304186569622	36.0	36.0	36.0	32.0	36.0
33	34.48094040718075	36.0	36.0	36.0	32.0	36.0
34	34.476697603205636	36.0	36.0	36.0	32.0	36.0
35	34.433848493827526	36.0	36.0	36.0	32.0	36.0
36	34.42466202052875	36.0	36.0	36.0	32.0	36.0
37	34.41100707195153	36.0	36.0	36.0	32.0	36.0
38	33.98063813606512	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	17.0
22	71.0
23	244.0
24	637.0
25	1621.0
26	3534.0
27	7018.0
28	12386.0
29	20020.0
30	31102.0
31	44961.0
32	65428.0
33	104900.0
34	252499.0
35	545641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20358020647839	18.13892382795119	11.961544707167844	26.695951258402577
2	15.670945553587302	20.418482663214935	37.02284509132808	26.887726691869684
3	18.2152518941253	24.202788600113205	28.705664991867575	28.876294513893924
4	12.11561342689213	16.064310817269543	36.08098847700308	35.73908727883524
5	14.023269830407099	37.16246774322275	33.60731908913191	15.20694333723824
6	34.526700309426545	35.919257376286716	16.369700967175834	13.184341347110903
7	30.18351081396458	30.53660675625096	20.740464850028257	18.539417579756197
8	27.846820424799578	33.78425362844669	19.13276890319586	19.23615704355787
9	27.152839765989594	14.200160178049002	18.279838207326907	40.367161848634495
10	15.166245899353436	27.341863656712583	32.26768507034398	25.224205373590003
11	37.628161375078896	21.26854991413096	21.8681653382653	19.235123372524843
12	24.19395676272707	23.985623047158093	29.005447316614042	22.814972873500796
13	29.612009415839967	19.59006603197202	25.09251631534624	25.705408236841766
14	23.0714965218181	19.63881583111714	25.553697385790596	31.73599026127416
15	25.025479757926245	27.415026956712392	22.44686404037865	25.112629244982713
16	25.487647248232015	26.224931908729722	23.847126956620652	24.440293886417617
17	24.021792875942246	26.18144890150365	25.23234511930765	24.564413103246455
18	24.640645970345325	25.29775310275108	26.16356032258153	23.898040604322063
19	25.195742334743933	25.5240665601914	25.579842231907534	23.700348873157132
20	25.48370258723893	24.561661014181517	25.40912097357903	24.545515425000527
21	26.261925730221634	24.716351093497725	24.848543226185235	24.173179950095406
22	25.309220140521667	24.757793228209646	25.275369445022893	24.65761718624579
23	24.22822178188757	24.56260091002495	25.66591442829884	25.543262879788642
24	24.38552731402529	25.3031655445788	25.60158373552057	24.709723405875344
25	24.698348104406918	24.803202697781174	25.538193950724764	24.960255247087144
26	24.312321744897854	25.678825701943246	25.737078253817835	24.271774299341057
27	25.050432032115044	25.209135834859982	25.104556450392217	24.635875682632758
28	24.3319533135611	25.107767220967986	25.75148085325769	24.80879861221322
29	24.24333604567	25.352703147747736	25.841749374587756	24.562211431994506
30	24.405525827897193	25.31197222958661	25.91495494371519	24.367546998801007
31	24.633307066172147	25.30711020557188	25.141709652768924	24.91787307548705
32	24.447082372777803	25.291239825297385	25.12391281014897	25.13776499177584
33	24.117749048006523	24.991996007636132	25.529570738321283	25.360684206036066
34	24.828154971969973	25.154460998769817	25.613234245895487	24.404149783364723
35	25.173542149620076	25.016397864011942	25.586905927174218	24.223154059193767
36	24.347640221231266	25.6220409309033	25.305458952132913	24.724859895732518
37	25.090979477671844	25.4975547688658	25.176294238685017	24.235171514777342
38	24.41022677234698	25.235762512843095	25.588121972699252	24.76588874211067
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	53.5
2	61.0
3	61.0
4	219.5
5	378.0
6	378.0
7	496.5
8	615.0
9	681.0
10	747.0
11	747.0
12	1016.0
13	1285.0
14	1804.0
15	2323.0
16	2323.0
17	3466.0
18	4609.0
19	4609.0
20	5673.0
21	6737.0
22	7823.5
23	8910.0
24	8910.0
25	10590.0
26	12270.0
27	12270.0
28	15712.5
29	19155.0
30	22750.5
31	26346.0
32	26346.0
33	32068.5
34	37791.0
35	37791.0
36	42580.5
37	47370.0
38	54012.0
39	60654.0
40	60654.0
41	66161.0
42	71668.0
43	79866.5
44	88065.0
45	88065.0
46	92156.0
47	96247.0
48	96247.0
49	100397.0
50	104547.0
51	104141.0
52	103735.0
53	103735.0
54	99036.5
55	94338.0
56	94338.0
57	89879.0
58	85420.0
59	76348.0
60	67276.0
61	67276.0
62	61514.0
63	55752.0
64	46222.0
65	36692.0
66	36692.0
67	30287.0
68	23882.0
69	23882.0
70	18956.0
71	14030.0
72	10820.0
73	7610.0
74	7610.0
75	5816.0
76	4022.0
77	4022.0
78	3695.0
79	3368.0
80	2594.5
81	1821.0
82	1821.0
83	1683.5
84	1546.0
85	1546.0
86	980.0
87	414.0
88	341.5
89	269.0
90	269.0
91	157.5
92	46.0
93	32.0
94	18.0
95	18.0
96	16.0
97	14.0
98	14.0
99	9.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008072794590493735
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	8.256267194823137E-4
8	0.0012843082303058214
9	0.0040363972952468675
10	8.256267194823137E-4
11	0.004495078806070374
12	2.7520890649410454E-4
13	2.7520890649410454E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	9.173630216470152E-5
22	0.0
23	9.173630216470152E-5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.173630216470152E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1090081.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.47236780189893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.56724760462002	48.91187109735964
2	13.128258570533971	16.14050278892592
3	3.6316957878625	6.69746817648272
4	1.366829857701358	3.3608907094094023
5	0.6700190176514529	2.059382774366856
6	0.3822077027965063	1.4097127489815424
7	0.2420995310656152	1.0417701992832926
8	0.17813893266361766	0.8760497590828489
9	0.1204916228901702	0.6666214823417022
>10	0.59585557986749	6.6259615180919775
>50	0.056494532343442296	2.493676648796512
>100	0.05437046474239291	6.7538008096495155
>500	0.005541891063657837	2.305620741723756
>1k	7.489041977915996E-4	0.656670545504326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2294	0.2104430771658253	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1609	0.14760371018300475	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1115	0.10228597691364219	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1104	0.10127687758983049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	9.173630216470152E-5	0.0
6	0.0	0.0	0.0	9.173630216470152E-5	0.0
7	0.0	0.0	0.0	9.173630216470152E-5	0.0
8	0.0	0.0	0.0	9.173630216470152E-5	0.0
9	0.0	0.0	0.0	9.173630216470152E-5	0.0
10	0.0	0.0	0.0	9.173630216470152E-5	0.0
11	0.0	0.0	0.0	9.173630216470152E-5	0.0
12	0.0	0.0	0.0	9.173630216470152E-5	1.8347260432940304E-4
13	0.0	0.0	0.0	9.173630216470152E-5	2.752089064941046E-4
14	0.0	0.0	0.0	9.173630216470152E-5	2.752089064941046E-4
15	0.0	0.0	0.0	9.173630216470152E-5	4.5868151082350763E-4
16	0.0	0.0	0.0	1.8347260432940304E-4	5.504178129882092E-4
17	0.0	0.0	0.0	2.752089064941046E-4	5.504178129882092E-4
18	0.0	0.0	0.0	2.752089064941046E-4	5.504178129882092E-4
19	0.0	0.0	0.0	3.669452086588061E-4	5.504178129882092E-4
20	0.0	0.0	0.0	3.669452086588061E-4	7.338904173176122E-4
21	0.0	0.0	0.0	4.5868151082350763E-4	7.338904173176122E-4
22	0.0	0.0	0.0	6.421541151529107E-4	7.338904173176122E-4
23	0.0	0.0	0.0	8.256267194823137E-4	7.338904173176122E-4
24	0.0	9.173630216470152E-5	0.0	0.0013760445324705228	7.338904173176122E-4
25	0.0	9.173630216470152E-5	0.0	0.0016512534389646273	7.338904173176122E-4
26	0.0	9.173630216470152E-5	0.0	0.0025686164606116427	7.338904173176122E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAACG	75	6.401751E-8	21.333302	15
CGAACGA	80	1.2653618E-7	19.999971	16
TTTTCGG	240	0.0	19.333303	29
TTTCGGA	235	0.0	19.063803	30
CTAGCGG	190	0.0	18.526289	29
TAACGAA	90	4.366284E-7	17.777752	13
TCTAGCG	200	0.0	17.599976	28
TTCGGAA	250	0.0	17.279976	31
GCGTAAC	165	1.8189894E-12	16.484825	11
ATCGTTT	175	0.0	16.457119	29
TAGCGGC	215	0.0	16.37207	30
AACGAAC	100	1.3142362E-6	15.999977	14
GTTTTCG	270	0.0	15.999977	28
CGCGTAA	150	1.1459633E-10	15.999976	10
TCGCGTA	150	1.1459633E-10	15.999976	9
ACGCTGA	275	0.0	15.709069	11
CGTTTAT	175	3.6379788E-12	15.542835	31
GTCCTAT	280	0.0	15.429257	1
CGATAAC	105	2.18461E-6	15.238073	10
ACACGCT	275	0.0	15.127252	9
>>END_MODULE