FastQCFastQC Report
Thu 2 Feb 2017
SRR4063068_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063068_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970220
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT61190.20601167590279507No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC58150.1957767438102228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT56490.1901879322070419No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT47310.15928113069065591No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA43690.14709348129094815No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC43500.14645379803516237No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG41560.13992229531819192No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC41550.13988862777841374No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG40560.1365555413403721No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC38740.13042804910074002No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG36700.12355987098598756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35930.12096747042306631No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG35850.1206981301048407No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC35720.12026045208772414No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG35580.11978910653082937No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG33450.11261792055807314No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG33280.11204557238184377No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG33130.11154055928517079No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC32740.11022752523382107No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC32330.10884715610291493No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA32220.10847681316535475No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA30450.10251765862461368No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC29910.10069961147659097No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT29710.100026260681027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG10400.018.92277729
TCTAGCG10550.018.80475628
CAAGACG13500.018.4848614
AAGACGG13750.018.0315255
ATCGTTT7800.017.84587329
TAGCGGC11000.017.74547430
CGCAAGA12550.017.217552
GCGCAAG12700.017.1512051
CATCGTT8050.017.09232128
TACCGTC8600.016.9288257
AGCGGCG11550.016.90045231
GCAAGAC15350.016.8843063
AAACGCT7900.016.8098629
ACCGTCG8350.016.6692738
GACGGAC14600.016.546577
CGCTTCG8050.016.49691232
TAAACGC8250.016.48403428
ATACCGT8850.016.4481186
TCGTTTA8400.016.38096830
AACGCTT8200.016.19513930
Produced by FastQC (version 0.11.5)