##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063068_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2970220
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25269575991004	32.0	32.0	32.0	32.0	32.0
2	30.85473735952219	32.0	32.0	32.0	32.0	32.0
3	30.90096693174243	32.0	32.0	32.0	32.0	32.0
4	30.97289157032139	32.0	32.0	32.0	32.0	32.0
5	30.863359616459388	32.0	32.0	32.0	32.0	32.0
6	34.520202207243905	36.0	36.0	36.0	32.0	36.0
7	34.45838086067699	36.0	36.0	36.0	32.0	36.0
8	34.43640908754234	36.0	36.0	36.0	32.0	36.0
9	34.59246688797463	36.0	36.0	36.0	32.0	36.0
10	34.29451724114712	36.0	36.0	36.0	32.0	36.0
11	34.56372760266916	36.0	36.0	36.0	32.0	36.0
12	34.37612096073691	36.0	36.0	36.0	32.0	36.0
13	34.47005609012127	36.0	36.0	36.0	32.0	36.0
14	34.36232736968979	36.0	36.0	36.0	32.0	36.0
15	34.291442721414576	36.0	36.0	36.0	32.0	36.0
16	34.320691396596885	36.0	36.0	36.0	32.0	36.0
17	34.23065362161726	36.0	36.0	36.0	32.0	36.0
18	34.2670761761755	36.0	36.0	36.0	32.0	36.0
19	34.26293877221216	36.0	36.0	36.0	32.0	36.0
20	34.24867383560814	36.0	36.0	36.0	32.0	36.0
21	34.23382948064453	36.0	36.0	36.0	32.0	36.0
22	34.21315626451913	36.0	36.0	36.0	32.0	36.0
23	34.147716330776845	36.0	36.0	36.0	32.0	36.0
24	34.13006410299574	36.0	36.0	36.0	32.0	36.0
25	34.1083030886601	36.0	36.0	36.0	32.0	36.0
26	34.05501646342695	36.0	36.0	36.0	32.0	36.0
27	34.05791826868043	36.0	36.0	36.0	32.0	36.0
28	34.047512978836586	36.0	36.0	36.0	32.0	36.0
29	34.011404542424465	36.0	36.0	36.0	32.0	36.0
30	33.98552363124617	36.0	36.0	36.0	32.0	36.0
31	34.01100356202571	36.0	36.0	36.0	32.0	36.0
32	33.96242668893213	36.0	36.0	36.0	32.0	36.0
33	33.92836254553535	36.0	36.0	36.0	32.0	36.0
34	33.945503363387225	36.0	36.0	36.0	32.0	36.0
35	33.90390341456188	36.0	36.0	36.0	32.0	36.0
36	33.86010766879221	36.0	36.0	36.0	32.0	36.0
37	33.862052979240595	36.0	36.0	36.0	32.0	36.0
38	33.25911043626398	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	5.0
8	8.0
9	13.0
10	28.0
11	38.0
12	11.0
13	16.0
14	170.0
15	472.0
16	706.0
17	843.0
18	1143.0
19	1593.0
20	2324.0
21	3486.0
22	5399.0
23	8241.0
24	12720.0
25	19438.0
26	28808.0
27	41517.0
28	58806.0
29	79081.0
30	106624.0
31	144998.0
32	199024.0
33	299280.0
34	653203.0
35	1302224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05881690687762	16.993775571359134	11.209904075170442	25.737503446592815
2	17.24906186629658	18.731043635849172	37.15076836013555	26.869126137718702
3	19.780705239665927	22.35693380616886	27.567642372564045	30.294718581601167
4	12.663553887685422	14.443682784747564	35.30999736711152	37.5827659604555
5	15.192652915010779	35.83936096143979	33.14568118370002	15.82230493984941
6	35.94410095979681	34.576218801890235	16.00407647669594	13.475603761617016
7	31.76355009512767	29.527317682635523	19.84745905941616	18.861673162820654
8	28.86911325095305	32.65315673072165	18.610340215116175	19.867389803209136
9	26.999012866404325	14.314177824104373	18.315233639573687	40.37157566991761
10	16.23456420194424	25.82692917595615	30.655752496628182	27.28275412547143
11	38.93020181815795	20.86689927003662	21.147794268907045	19.05510464289839
12	25.211034675946625	23.27398011129487	27.801663766679415	23.713321446079092
13	29.336239486213	18.607807754821803	25.25133923565665	26.80461352330854
14	23.548540678572174	19.61772317439196	24.262190978813727	32.57154516822214
15	25.47087941156364	26.919710644597384	21.699297894559248	25.910112049279725
16	26.594136336638062	25.526653566267232	22.99227848629778	24.88693161079692
17	24.47843722270957	25.831791310907082	24.41169388988697	25.278077576496376
18	25.788136472052265	24.245196367461222	25.607465747982182	24.359201412504326
19	25.67728319715447	24.773509414674994	24.983914092668968	24.56529329550157
20	25.914366021152606	24.000483495822948	24.508692823925365	25.576457659099084
21	27.36385261453148	23.879954276633104	24.111264682066036	24.64492842676938
22	26.016342832477047	23.847545460636695	24.932836406354642	25.20327530053162
23	24.357745805456695	23.683925210358495	25.581141643069028	26.377187341115782
24	24.897938567840043	24.84864198694853	24.95026567624538	25.30315376896605
25	25.142750349783395	24.25242322864806	24.972732890077936	25.632093531490607
26	24.69670637621239	25.555124902050647	25.409147955969182	24.33902076576778
27	25.71213272133691	24.21493680705951	24.688614496126537	25.384315975477044
28	24.704878208916025	24.31902546931107	25.639173817231725	25.336922504541175
29	24.474052823683113	24.690559887941603	25.616893611863105	25.218493676512182
30	24.407970048348215	24.98245862120049	25.83942736320418	24.770143967247115
31	25.27945713935551	24.843301984751744	24.320245760668865	25.55699511522388
32	24.962878912865612	24.530557136171257	24.27732566557015	26.22923828539298
33	24.4036283316947	24.16985413944969	25.197544748077416	26.228972780778193
34	25.700445711395727	24.440183860580586	25.08499561763707	24.77437481038662
35	26.171332285878417	24.102159853832223	25.478308187456268	24.248199672833092
36	24.461631035805286	25.150913020829652	24.80112009164386	25.586335851721202
37	25.94324391392814	24.691197153340287	24.724026132540857	24.64153280019071
38	24.644129132354536	24.20167688090186	25.368362290314128	25.785831696429472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	74.0
1	117.0
2	160.0
3	160.0
4	486.0
5	812.0
6	812.0
7	1026.0
8	1240.0
9	1239.0
10	1238.0
11	1238.0
12	1761.0
13	2284.0
14	3438.0
15	4592.0
16	4592.0
17	7032.0
18	9472.0
19	9472.0
20	11932.5
21	14393.0
22	16228.5
23	18064.0
24	18064.0
25	22284.5
26	26505.0
27	26505.0
28	33404.5
29	40304.0
30	49141.0
31	57978.0
32	57978.0
33	76093.5
34	94209.0
35	94209.0
36	106766.5
37	119324.0
38	137392.5
39	155461.0
40	155461.0
41	166816.5
42	178172.0
43	203137.5
44	228103.0
45	228103.0
46	232467.5
47	236832.0
48	236832.0
49	256330.0
50	275828.0
51	283802.0
52	291776.0
53	291776.0
54	278058.5
55	264341.0
56	264341.0
57	256499.0
58	248657.0
59	223607.0
60	198557.0
61	198557.0
62	189064.5
63	179572.0
64	149456.0
65	119340.0
66	119340.0
67	100197.0
68	81054.0
69	81054.0
70	64632.0
71	48210.0
72	37000.5
73	25791.0
74	25791.0
75	19085.5
76	12380.0
77	12380.0
78	12541.0
79	12702.0
80	9985.5
81	7269.0
82	7269.0
83	7443.5
84	7618.0
85	7618.0
86	5096.0
87	2574.0
88	2231.5
89	1889.0
90	1889.0
91	1353.5
92	818.0
93	719.5
94	621.0
95	621.0
96	580.0
97	539.0
98	539.0
99	1003.0
100	1467.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11857707509881424
2	0.052521362053989266
3	0.011312293365474612
4	0.0032994188982634286
5	2.69340318225586E-4
6	2.69340318225586E-4
7	3.366753977819825E-5
8	3.703429375601807E-4
9	1.34670159112793E-4
10	4.713455568947755E-4
11	0.0023903953242520757
12	0.003097413659594239
13	0.014443374564847048
14	0.009359576058339113
15	0.024341631259637335
16	0.011312293365474612
17	0.021378887759155887
18	0.005925487000962891
19	0.007709866609207399
20	0.006194827319188478
21	0.006228494858966677
22	0.007844536768320191
23	0.009763586535677491
24	0.014813717502407227
25	0.017675458383554082
26	0.019291500292907597
27	0.00902290066055713
28	0.005891819461184693
29	0.011446963524587404
30	0.0033667539778198248
31	0.005925487000962891
32	0.007070183353421633
33	0.008753560342331544
34	0.012153981859929568
35	0.016227754173091555
36	0.015588070917305788
37	0.009527913757230104
38	0.006127492239632081
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2970220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.344120371205975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68232638407873	34.610867235454364
2	14.13490567104311	13.10139539708928
3	4.938383307771386	6.865950913635343
4	2.1696615169138638	4.022042180185179
5	1.2028390451838666	2.787225874859379
6	0.696110495815583	1.9356377165842356
7	0.4347269849583048	1.410292780366339
8	0.2991279475905434	1.109025728762235
9	0.21678008599009496	0.904183415928481
>10	1.0513029149712945	8.550341523648529
>50	0.0764108311208883	2.470192822854288
>100	0.07069880288113892	7.110734511055716
>500	0.015188553086568834	4.851834711426388
>1k	0.011318392925059793	9.675242686714471
>5k	2.1906566951728631E-4	0.5950325014358185
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	6119	0.20601167590279507	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5815	0.1957767438102228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5649	0.1901879322070419	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4731	0.15928113069065591	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4369	0.14709348129094815	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	4350	0.14645379803516237	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	4156	0.13992229531819192	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	4155	0.13988862777841374	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	4056	0.1365555413403721	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3874	0.13042804910074002	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3670	0.12355987098598756	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3593	0.12096747042306631	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3585	0.1206981301048407	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3572	0.12026045208772414	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3558	0.11978910653082937	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3345	0.11261792055807314	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3328	0.11204557238184377	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	3313	0.11154055928517079	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	3274	0.11022752523382107	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	3233	0.10884715610291493	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	3222	0.10847681316535475	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3045	0.10251765862461368	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2991	0.10069961147659097	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2971	0.100026260681027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3667539778198247E-4
2	0.0	0.0	0.0	0.0	3.3667539778198247E-4
3	0.0	0.0	0.0	0.0	3.3667539778198247E-4
4	0.0	0.0	0.0	0.0	3.3667539778198247E-4
5	0.0	0.0	0.0	0.0	3.703429375601807E-4
6	0.0	0.0	0.0	0.0	4.713455568947755E-4
7	0.0	0.0	0.0	0.0	4.713455568947755E-4
8	0.0	0.0	0.0	0.0	4.713455568947755E-4
9	0.0	0.0	0.0	3.366753977819825E-5	7.406858751203614E-4
10	0.0	0.0	0.0	3.366753977819825E-5	7.406858751203614E-4
11	0.0	0.0	0.0	3.366753977819825E-5	7.743534148985597E-4
12	0.0	0.0	0.0	3.366753977819825E-5	0.0013130340513497316
13	0.0	0.0	0.0	6.73350795563965E-5	0.0014477042104625248
14	0.0	0.0	0.0	6.73350795563965E-5	0.0015823743695753177
15	0.0	0.0	0.0	6.73350795563965E-5	0.0019527173071354983
16	0.0	0.0	0.0	6.73350795563965E-5	0.0020200523866918947
17	0.0	0.0	0.0	6.73350795563965E-5	0.0020200523866918947
18	0.0	0.0	0.0	1.0100261933459474E-4	0.00212105500602649
19	0.0	0.0	0.0	1.0100261933459474E-4	0.0022557251651392825
20	0.0	0.0	0.0	1.34670159112793E-4	0.002323060244695679
21	0.0	0.0	0.0	3.3667539778198247E-4	0.0023903953242520757
22	0.0	0.0	0.0	5.050130966729737E-4	0.0023903953242520757
23	0.0	0.0	0.0	8.416884944549562E-4	0.002424062864030274
24	0.0	0.0	0.0	0.0013467015911279299	0.002457730403808472
25	0.0	0.0	0.0	0.0016160419093535158	0.002457730403808472
26	0.0	0.0	0.0	0.0022557251651392825	0.002558733023143067
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	1040	0.0	18.922777	29
TCTAGCG	1055	0.0	18.804756	28
CAAGACG	1350	0.0	18.484861	4
AAGACGG	1375	0.0	18.031525	5
ATCGTTT	780	0.0	17.845873	29
TAGCGGC	1100	0.0	17.745474	30
CGCAAGA	1255	0.0	17.21755	2
GCGCAAG	1270	0.0	17.151205	1
CATCGTT	805	0.0	17.092321	28
TACCGTC	860	0.0	16.928825	7
AGCGGCG	1155	0.0	16.900452	31
GCAAGAC	1535	0.0	16.884306	3
AAACGCT	790	0.0	16.80986	29
ACCGTCG	835	0.0	16.669273	8
GACGGAC	1460	0.0	16.54657	7
CGCTTCG	805	0.0	16.496912	32
TAAACGC	825	0.0	16.484034	28
ATACCGT	885	0.0	16.448118	6
TCGTTTA	840	0.0	16.380968	30
AACGCTT	820	0.0	16.195139	30
>>END_MODULE