FastQCFastQC Report
Thu 2 Feb 2017
SRR4063068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970220
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC62570.21065779639218643No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT61850.20823373352815616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT47560.16012281918511087No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT46840.15769875632108057No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC45890.15450034004215177No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC44870.15106625098477555No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA43620.14685780851250077No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC40680.13695955181771047No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG39780.13392947323767262No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG39740.13379480307855984No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG39410.1326837742658793No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG37540.12638794432735623No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC36860.12409855162243874No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG35590.11982277407060757No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG35180.11844240493970144No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA34270.11537865881988539No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG34190.11510931850165981No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA33470.11268525563762953No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG33460.11265158809785135No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC33170.1116752294442836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33110.1114732242056144No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC31220.10511005918753492No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT30970.10426837069307997No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG30900.1040326979146326No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG30800.1036960225168506No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT29850.10049760623792178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG10850.021.23418628
CTAGCGG10850.021.23418629
TAGCGGC11100.020.75593830
AGCGGCG12700.018.26699631
AAGACGG14050.017.7671175
CAAGACG14150.017.7534454
TAAACGC7850.017.52797128
GCGGCGC13800.017.04280532
CGCAAGA13700.016.8177032
GCGCAAG13950.016.631851
AACGCTT8450.016.47272330
CGGTCCA14500.016.44211410
AAACGCT8450.016.2833829
CGCTTCG8650.016.0918532
GACGGAC15050.016.0549557
CCGGTCC14950.015.947479
GTATCAA42800.015.8140571
CGACCCG7350.015.6752265
CGGACCA15300.015.5826589
ACGCTTC9050.015.55739731
Produced by FastQC (version 0.11.5)