##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2970220
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.165866164795872	32.0	32.0	32.0	32.0	32.0
2	31.184304192955405	32.0	32.0	32.0	32.0	32.0
3	31.313960918719825	32.0	32.0	32.0	32.0	32.0
4	31.427616472853863	32.0	32.0	32.0	32.0	32.0
5	31.32476584226084	32.0	32.0	32.0	32.0	32.0
6	34.853695012490654	36.0	36.0	36.0	32.0	36.0
7	34.88453919238306	36.0	36.0	36.0	32.0	36.0
8	34.8073920450337	36.0	36.0	36.0	32.0	36.0
9	34.94541885786238	36.0	36.0	36.0	32.0	36.0
10	34.760227188558424	36.0	36.0	36.0	32.0	36.0
11	34.958821568772684	36.0	36.0	36.0	36.0	36.0
12	34.84356714317458	36.0	36.0	36.0	32.0	36.0
13	34.91171529381662	36.0	36.0	36.0	32.0	36.0
14	34.847808243160436	36.0	36.0	36.0	32.0	36.0
15	34.81655735938752	36.0	36.0	36.0	32.0	36.0
16	34.82885005151134	36.0	36.0	36.0	32.0	36.0
17	34.78362882210745	36.0	36.0	36.0	32.0	36.0
18	34.787198254674735	36.0	36.0	36.0	32.0	36.0
19	34.79202988330831	36.0	36.0	36.0	32.0	36.0
20	34.78039370821017	36.0	36.0	36.0	32.0	36.0
21	34.76760879665479	36.0	36.0	36.0	32.0	36.0
22	34.75439260391486	36.0	36.0	36.0	32.0	36.0
23	34.69202954663291	36.0	36.0	36.0	32.0	36.0
24	34.66185770750988	36.0	36.0	36.0	32.0	36.0
25	34.64446471978507	36.0	36.0	36.0	32.0	36.0
26	34.5758206462821	36.0	36.0	36.0	32.0	36.0
27	34.56203075866434	36.0	36.0	36.0	32.0	36.0
28	34.53717771747547	36.0	36.0	36.0	32.0	36.0
29	34.49317356963457	36.0	36.0	36.0	32.0	36.0
30	34.46831345826235	36.0	36.0	36.0	32.0	36.0
31	34.47410831520897	36.0	36.0	36.0	32.0	36.0
32	34.45449697328817	36.0	36.0	36.0	32.0	36.0
33	34.41993556032887	36.0	36.0	36.0	32.0	36.0
34	34.41680717253267	36.0	36.0	36.0	32.0	36.0
35	34.38487990788561	36.0	36.0	36.0	32.0	36.0
36	34.35859195615139	36.0	36.0	36.0	32.0	36.0
37	34.36337039007211	36.0	36.0	36.0	32.0	36.0
38	33.89636895583492	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	59.0
22	216.0
23	658.0
24	1897.0
25	4760.0
26	10252.0
27	20063.0
28	35196.0
29	56535.0
30	86837.0
31	126974.0
32	184671.0
33	299841.0
34	714353.0
35	1427899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.193064931418014	18.28112199906463	11.978421178276125	26.54739189124123
2	16.18378436614123	19.763350862899046	36.33373958831332	27.719125182646405
3	18.63663297668186	23.14448088020416	27.940118913750496	30.278767229363478
4	12.136954257229604	15.408094624704315	35.304344664263695	37.15060645380238
5	14.710810691609403	36.37044027422912	32.744442577764524	16.174306456396955
6	35.99378900807954	35.04951488409268	15.614039104200433	13.342657003627343
7	31.54923830250272	29.72761503571467	19.76659547075491	18.9565511910277
8	28.753723725179654	32.93890801254081	18.33207413512611	19.975294127153433
9	26.91580446515449	14.103046688506488	18.06545885505924	40.915689991279784
10	15.939288711930452	26.227960577592874	30.579367171584032	27.253383538892646
11	39.185555646088275	20.771098632559323	20.96924184380591	19.074103877546484
12	25.13979627737386	23.443218756891337	27.55840907139719	23.858575894337616
13	29.39261972618144	18.624207338526	25.144846416841876	26.838326518450685
14	23.45729388977715	19.551420282477487	24.234206299266148	32.75707952847921
15	25.5015790076156	27.04335032421841	21.449656927769663	26.00541374039633
16	26.63268264057297	25.680968305704056	22.70866222322327	24.977686830499703
17	24.49323619125856	25.97948973476712	24.16251994801732	25.364754125957
18	25.831790237760167	24.372605396233276	25.35936731959249	24.43623704641407
19	25.68853485600393	24.895630626687584	24.765034239887953	24.65080027742053
20	26.02867801038307	23.950448114954447	24.288537549407117	25.732336325255368
21	27.405294830211112	23.955851052010324	23.907235091835012	24.731619025943548
22	25.972082876015918	23.972231013190942	24.779073603975462	25.276612506817674
23	24.35304368436504	23.674170498037853	25.409431502624724	26.563354314972383
24	24.965894782204685	24.911387035303783	24.7394805771963	25.38323760529523
25	25.1275495603353	24.366258638254834	24.874201152912008	25.631990648497855
26	24.849253489137833	25.53347936077419	25.168960662146684	24.44830648794129
27	25.76489386630467	24.259212124640094	24.465156742809278	25.510737266245957
28	24.702547286059616	24.44263387897193	25.5261226441139	25.32869619085455
29	24.568483142662835	24.668240063025635	25.452323396920097	25.31095339739144
30	24.4962662698386	24.91293574213358	25.690521240850845	24.900276747176978
31	25.335396031270413	24.80381924571244	24.254769007009582	25.60601571600757
32	25.15561136885483	24.45862596036657	24.21069819743992	26.175064473338676
33	24.382907663405405	24.242446687450762	25.075011278625826	26.29963437051801
34	25.663317868710063	24.407585970062822	25.099958925601474	24.829137235625645
35	26.173044420951985	24.150870979254062	25.31452215660793	24.361562443186028
36	24.551245362296395	25.15022456249032	24.68308744806782	25.615442627145462
37	26.008174478658148	24.766852287035977	24.52552336190585	24.699449872400024
38	24.7359368019239	24.1262170407078	25.26414736701056	25.87369879035774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	60.0
2	63.0
3	63.0
4	274.0
5	485.0
6	485.0
7	704.5
8	924.0
9	978.0
10	1032.0
11	1032.0
12	1511.0
13	1990.0
14	3102.0
15	4214.0
16	4214.0
17	6576.5
18	8939.0
19	8939.0
20	11194.0
21	13449.0
22	15740.5
23	18032.0
24	18032.0
25	22265.0
26	26498.0
27	26498.0
28	33363.5
29	40229.0
30	49294.5
31	58360.0
32	58360.0
33	76774.5
34	95189.0
35	95189.0
36	107478.0
37	119767.0
38	137742.5
39	155718.0
40	155718.0
41	166825.0
42	177932.0
43	202846.0
44	227760.0
45	227760.0
46	231910.5
47	236061.0
48	236061.0
49	255888.5
50	275716.0
51	283615.0
52	291514.0
53	291514.0
54	278732.5
55	265951.0
56	265951.0
57	258210.5
58	250470.0
59	224949.0
60	199428.0
61	199428.0
62	190456.5
63	181485.0
64	151051.5
65	120618.0
66	120618.0
67	101457.5
68	82297.0
69	82297.0
70	65625.5
71	48954.0
72	37331.5
73	25709.0
74	25709.0
75	18875.0
76	12041.0
77	12041.0
78	12237.5
79	12434.0
80	9790.5
81	7147.0
82	7147.0
83	6765.0
84	6383.0
85	6383.0
86	4089.5
87	1796.0
88	1502.5
89	1209.0
90	1209.0
91	724.0
92	239.0
93	157.5
94	76.0
95	76.0
96	49.0
97	22.0
98	22.0
99	27.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009123903279891725
2	0.0
3	0.0
4	6.73350795563965E-5
5	1.0100261933459474E-4
6	6.73350795563965E-5
7	4.0401047733837895E-4
8	0.002558733023143067
9	0.003703429375601807
10	0.0010436937331241456
11	0.004915460807616945
12	1.6833769889099123E-4
13	1.6833769889099123E-4
14	3.366753977819825E-5
15	0.0
16	3.366753977819825E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.73350795563965E-5
22	0.0
23	1.34670159112793E-4
24	0.0
25	6.73350795563965E-5
26	6.73350795563965E-5
27	1.34670159112793E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0100261933459474E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2970220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.1251240393757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48519851912658	31.984183756752323
2	15.227894016661997	13.438654246873552
3	5.37992982259253	7.121702122350956
4	2.4478499624391117	4.320467328896278
5	1.272817854534149	2.808162285542569
6	0.7669920811806465	2.030617243158897
7	0.4828765391509339	1.491489103001759
8	0.35345916892618595	1.2477143737378082
9	0.23439574754730458	0.9308467291344306
>10	1.1641163272077584	8.97436188036986
>50	0.07860067415364581	2.4149428866428138
>100	0.07675394401062369	7.296116562276252
>500	0.016670262046584748	5.14243163686702
>1k	0.012291437453688749	10.376556214483209
>5k	1.5364296817110937E-4	0.4217536299122882
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	6257	0.21065779639218643	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	6185	0.20823373352815616	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4756	0.16012281918511087	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4684	0.15769875632108057	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	4589	0.15450034004215177	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	4487	0.15106625098477555	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4362	0.14685780851250077	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	4068	0.13695955181771047	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3978	0.13392947323767262	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3974	0.13379480307855984	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3941	0.1326837742658793	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	3754	0.12638794432735623	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3686	0.12409855162243874	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3559	0.11982277407060757	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3518	0.11844240493970144	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	3427	0.11537865881988539	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3419	0.11510931850165981	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3347	0.11268525563762953	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3346	0.11265158809785135	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	3317	0.1116752294442836	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3311	0.1114732242056144	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	3122	0.10511005918753492	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	3097	0.10426837069307997	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	3090	0.1040326979146326	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	3080	0.1036960225168506	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2985	0.10049760623792178	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.69340318225586E-4
2	0.0	0.0	0.0	0.0	2.69340318225586E-4
3	0.0	0.0	0.0	0.0	2.69340318225586E-4
4	0.0	0.0	0.0	0.0	3.030078580037842E-4
5	0.0	0.0	0.0	0.0	3.030078580037842E-4
6	0.0	0.0	0.0	0.0	4.0401047733837895E-4
7	0.0	0.0	0.0	0.0	4.0401047733837895E-4
8	0.0	0.0	0.0	0.0	4.3767801711657725E-4
9	0.0	0.0	0.0	3.366753977819825E-5	4.713455568947755E-4
10	0.0	0.0	0.0	3.366753977819825E-5	4.713455568947755E-4
11	0.0	0.0	0.0	3.366753977819825E-5	5.050130966729737E-4
12	0.0	0.0	0.0	3.366753977819825E-5	0.0010100261933459473
13	0.0	0.0	0.0	3.366753977819825E-5	0.0010436937331241456
14	0.0	0.0	0.0	3.366753977819825E-5	0.0011446963524587405
15	0.0	0.0	0.0	6.73350795563965E-5	0.0013803691309061282
16	0.0	0.0	0.0	6.73350795563965E-5	0.0014813717502407228
17	0.0	0.0	0.0	6.73350795563965E-5	0.0015150392900189211
18	0.0	0.0	0.0	1.0100261933459474E-4	0.0015150392900189211
19	0.0	0.0	0.0	1.0100261933459474E-4	0.0015150392900189211
20	0.0	0.0	0.0	1.6833769889099123E-4	0.0016160419093535158
21	0.0	0.0	0.0	3.3667539778198247E-4	0.0016160419093535158
22	0.0	0.0	0.0	5.38680636451172E-4	0.0016160419093535158
23	0.0	0.0	0.0	8.416884944549562E-4	0.0018180471480227054
24	0.0	0.0	0.0	0.0013130340513497316	0.0018517146878009037
25	0.0	0.0	0.0	0.0015487068297971194	0.0018517146878009037
26	0.0	0.0	0.0	0.00212105500602649	0.00212105500602649
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	1085	0.0	21.234186	28
CTAGCGG	1085	0.0	21.234186	29
TAGCGGC	1110	0.0	20.755938	30
AGCGGCG	1270	0.0	18.266996	31
AAGACGG	1405	0.0	17.767117	5
CAAGACG	1415	0.0	17.753445	4
TAAACGC	785	0.0	17.527971	28
GCGGCGC	1380	0.0	17.042805	32
CGCAAGA	1370	0.0	16.817703	2
GCGCAAG	1395	0.0	16.63185	1
AACGCTT	845	0.0	16.472723	30
CGGTCCA	1450	0.0	16.442114	10
AAACGCT	845	0.0	16.28338	29
CGCTTCG	865	0.0	16.09185	32
GACGGAC	1505	0.0	16.054955	7
CCGGTCC	1495	0.0	15.94747	9
GTATCAA	4280	0.0	15.814057	1
CGACCCG	735	0.0	15.675226	5
CGGACCA	1530	0.0	15.582658	9
ACGCTTC	905	0.0	15.557397	31
>>END_MODULE