FastQCFastQC Report
Thu 2 Feb 2017
SRR4063067_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063067_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789463
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT181881.0163943037659902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT118170.6603657074776065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT64450.3601639150963166No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53850.3009282673070077No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA50540.2824310980444971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29420.16440686395862894No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA29220.1632892102267552No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA27700.15479504186451465No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26610.14870382902580273No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25680.14350673917258977No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG24850.13886847618531367No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24500.1369125821545346No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24470.13674493409475355No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC24380.13624198991541037No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23650.13216255379407119No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23010.12858606185207516No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA22690.12679781588107716No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT22090.12344485468545592No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA22020.1230536758793001No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21780.12171249140105159No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21770.12165660871445792No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT21220.1185830609518051No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT21140.11813599945905559No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21020.11746540721993134No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.11685069766740079No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20570.1149506863232154No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20320.11355361915837321No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT19930.11137419438121939No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19550.10925065229065926No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC19320.10796535049900444No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT19290.10779770243922338No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT19160.10707122751350544No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18910.10567416034866325No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC18540.10360650094469682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG507.212138E-419.1944334
CGTAAAC1900.016.842432
GTATTAG4650.016.530281
CATCGTT4700.016.3393428
CCCACGA703.6941952E-416.002072
GGTTCCG2600.015.9975978
ATCGTTT4950.015.51542329
GTGTTAC951.4217096E-515.1708811
TATTAGC4300.014.8856452
AACGAAT1401.27947715E-814.85740331
TAATACT2400.014.6624154
TAGGACG1452.0396328E-814.3406694
TTAGGCA1901.8189894E-1114.3116394
ATTAGCT4250.014.3025333
GATATAC2250.014.2344071
CGGTGTT1351.3182398E-714.22366314
GTCGTAA2053.6379788E-1214.049026530
TACCGTC4100.014.046677
TCTAGCG5050.013.9396728
TATACAC3450.013.9097873
Produced by FastQC (version 0.11.5)