##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063067_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1789463
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0189649073493	32.0	32.0	32.0	32.0	32.0
2	30.709406117924765	32.0	32.0	32.0	32.0	32.0
3	30.80033339610822	32.0	32.0	32.0	32.0	32.0
4	30.8226305880591	32.0	32.0	32.0	32.0	32.0
5	30.81062083988325	32.0	32.0	32.0	32.0	32.0
6	34.41374758796354	36.0	36.0	36.0	32.0	36.0
7	34.2622144185155	36.0	36.0	36.0	32.0	36.0
8	34.23420322186041	36.0	36.0	36.0	32.0	36.0
9	34.294794583626484	36.0	36.0	36.0	32.0	36.0
10	34.11025374651501	36.0	36.0	36.0	32.0	36.0
11	34.402742051665776	36.0	36.0	36.0	32.0	36.0
12	34.227849919221576	36.0	36.0	36.0	32.0	36.0
13	34.30953531869617	36.0	36.0	36.0	32.0	36.0
14	34.21347912753714	36.0	36.0	36.0	32.0	36.0
15	34.201831499170424	36.0	36.0	36.0	32.0	36.0
16	34.19042584283665	36.0	36.0	36.0	32.0	36.0
17	34.107771437576524	36.0	36.0	36.0	32.0	36.0
18	34.14515192546591	36.0	36.0	36.0	32.0	36.0
19	34.11543798335031	36.0	36.0	36.0	32.0	36.0
20	34.04866990823504	36.0	36.0	36.0	32.0	36.0
21	34.00636783213735	36.0	36.0	36.0	32.0	36.0
22	34.008239343311374	36.0	36.0	36.0	32.0	36.0
23	33.995245501024606	36.0	36.0	36.0	32.0	36.0
24	33.98108650472236	36.0	36.0	36.0	32.0	36.0
25	33.95978849520778	36.0	36.0	36.0	32.0	36.0
26	33.91660459031564	36.0	36.0	36.0	32.0	36.0
27	33.9359623529517	36.0	36.0	36.0	32.0	36.0
28	33.92962413863824	36.0	36.0	36.0	32.0	36.0
29	33.89317297982691	36.0	36.0	36.0	32.0	36.0
30	33.848725008564024	36.0	36.0	36.0	32.0	36.0
31	33.860974493465356	36.0	36.0	36.0	32.0	36.0
32	33.80594513549595	36.0	36.0	36.0	32.0	36.0
33	33.71826240609613	36.0	36.0	36.0	27.0	36.0
34	33.696649777056024	36.0	36.0	36.0	27.0	36.0
35	33.63252160005543	36.0	36.0	36.0	27.0	36.0
36	33.586309971203654	36.0	36.0	36.0	27.0	36.0
37	33.582584831315316	36.0	36.0	36.0	27.0	36.0
38	32.90838592359831	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	8.0
10	11.0
11	20.0
12	9.0
13	10.0
14	780.0
15	1337.0
16	1515.0
17	1858.0
18	2330.0
19	2789.0
20	3381.0
21	4261.0
22	5777.0
23	7711.0
24	10234.0
25	13975.0
26	19242.0
27	26539.0
28	36418.0
29	48479.0
30	64225.0
31	85852.0
32	116411.0
33	173009.0
34	377824.0
35	785453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.139165076682914	17.818467684629287	12.060059421562977	23.982307817124823
2	15.935003900101485	21.203300128885076	38.20673613227355	24.65495983873989
3	18.104593537670652	27.39031461001961	28.49430844871344	26.010783403596292
4	11.998428524405654	15.964504322955001	36.268393725051006	35.76867342758834
5	13.802599666938631	37.79385960010283	32.39116828540454	16.01237244755401
6	31.653436381468104	36.490439826651134	17.849512840501717	14.00661095137905
7	28.500449576213647	31.99658221488793	21.70707078045201	17.795897428446413
8	28.536054751743407	31.130805455727124	20.295575366633155	20.037564425896317
9	28.688910273390043	14.108070711913886	19.489197287670347	37.713821727025724
10	16.680451132021275	26.429011307359673	30.379937120484797	26.51060044013425
11	35.93271846548298	22.572531233304797	22.740237706182285	18.754512595029944
12	25.83761683940401	24.734579098725735	28.290643570940784	21.137160490929475
13	29.10981432597944	20.260774162466024	26.190147500769896	24.43926401078464
14	24.610291914996356	20.39196596635086	26.244852701186172	28.752889417466616
15	24.7176283633205	26.739994511031878	24.769667358470425	23.7727097671772
16	24.575564995827886	26.523520045426572	24.751615331692804	24.149299627052734
17	22.667580349192917	25.955440090727333	26.264087413930042	25.112892146149708
18	23.98073493603554	24.463591029830337	28.44866272893051	23.107011305203613
19	24.75187542998106	24.86761752908782	27.929613602463053	22.45089343846807
20	25.55612311048916	23.412218501446333	27.141836821374444	23.889821566690063
21	27.44600981472074	23.50944726948791	26.033208651395224	23.011334264396126
22	25.506878820208424	23.893149011391397	26.666808246424782	23.933163921975396
23	23.19071988929681	23.942806332571205	27.777513861651194	25.08895991648079
24	23.675591628502797	25.695417093762963	27.202615226574352	23.426376051159895
25	24.014429069186424	24.580726439022428	27.310881159154597	24.093963332636548
26	23.389459152048335	25.544299340183947	28.063281977044774	23.002959530722947
27	24.217852304583236	25.35471892981852	26.75040127334427	23.67702749225397
28	22.894769932350304	25.207601579331218	28.071913779469256	23.82571470884923
29	22.881158901904676	25.4287199320397	28.047482786372175	23.642638379683447
30	23.13398095798003	25.65425159451909	27.994854172685873	23.21691327481501
31	23.553465674656287	25.755646003633696	26.468972477915326	24.22191584379469
32	23.010946549296914	25.96640867452696	26.426189452929616	24.59645532324651
33	22.83670249598031	25.289370189854566	27.134990613736974	24.73893670042815
34	23.702439357001456	25.59888489092029	27.316874967164868	23.381800784913388
35	24.720583196817746	25.42856392310861	27.033830713966733	22.81702216610691
36	23.171378541171684	26.592454852803176	26.45429277728687	23.78187382873827
37	24.263312792643354	26.561425903177454	26.400300900802215	22.774960403376973
38	23.04306470379813	26.441721425721237	26.929215795197596	23.585998075283037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3435.0
1	2430.0
2	1425.0
3	1425.0
4	3396.0
5	5367.0
6	5367.0
7	6105.0
8	6843.0
9	6359.0
10	5875.0
11	5875.0
12	7459.0
13	9043.0
14	10622.0
15	12201.0
16	12201.0
17	17548.5
18	22896.0
19	22896.0
20	25937.0
21	28978.0
22	25508.0
23	22038.0
24	22038.0
25	21857.0
26	21676.0
27	21676.0
28	28125.5
29	34575.0
30	41018.0
31	47461.0
32	47461.0
33	56145.5
34	64830.0
35	64830.0
36	69586.5
37	74343.0
38	84653.0
39	94963.0
40	94963.0
41	101008.0
42	107053.0
43	122665.5
44	138278.0
45	138278.0
46	142396.5
47	146515.0
48	146515.0
49	152883.0
50	159251.0
51	156158.5
52	153066.0
53	153066.0
54	143527.5
55	133989.0
56	133989.0
57	131165.5
58	128342.0
59	114977.5
60	101613.0
61	101613.0
62	98942.5
63	96272.0
64	78710.0
65	61148.0
66	61148.0
67	51578.0
68	42008.0
69	42008.0
70	33684.0
71	25360.0
72	19711.0
73	14062.0
74	14062.0
75	10452.5
76	6843.0
77	6843.0
78	6876.5
79	6910.0
80	5365.5
81	3821.0
82	3821.0
83	4089.0
84	4357.0
85	4357.0
86	2879.0
87	1401.0
88	1234.5
89	1068.0
90	1068.0
91	766.0
92	464.0
93	414.0
94	364.0
95	364.0
96	337.5
97	311.0
98	311.0
99	664.5
100	1018.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12478603916370441
2	0.05856505555018461
3	0.012964783289735524
4	0.0034647265688086313
5	1.676480597810628E-4
6	4.470614927495008E-4
7	0.0
8	2.235307463747504E-4
9	2.79413432968438E-4
10	5.029441793431885E-4
11	0.0015088325380295652
12	0.0022911901503411916
13	0.014082437021609277
14	0.00977947015389533
15	0.023470728369348794
16	0.011288302691924896
17	0.020453063293289664
18	0.006147095525305637
19	0.007823576123116264
20	0.006202978211899324
21	0.006147095525305637
22	0.007264749257179388
23	0.009723587467301642
24	0.013914788961828215
25	0.01659715791832522
26	0.018329521202729532
27	0.008885347168396328
28	0.005476503286181385
29	0.010226531646644832
30	0.0029058997028717556
31	0.0055882686593687605
32	0.007376514630366764
33	0.00815887224267839
34	0.012350073737204962
35	0.016205979112169405
36	0.014529498514358776
37	0.009444174034333204
38	0.006091212838711948
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1789463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.130845698167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79967053157966	25.91720796290978
2	14.849536621376489	11.027517059552208
3	6.116425488401704	6.813241531025382
4	3.015630812074588	4.478916894631182
5	1.7109247586195677	3.176404160673839
6	1.0174065136272457	2.2666298561881977
7	0.6989958033554317	1.8168013722639769
8	0.475383838972146	1.4121123177821615
9	0.3479295296237494	1.1627025910465736
>10	1.650866316975096	10.842267908764628
>50	0.13378130622699716	3.4965272898389084
>100	0.14748130315799815	11.917760212147304
>500	0.023777463637995502	6.043910008805374
>1k	0.011437260990428216	7.007922651618102
>5k	4.5147082856953485E-4	0.943449423907379
>10k+	3.009805523796899E-4	1.6766287588451143
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	18188	1.0163943037659902	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	11817	0.6603657074776065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	6445	0.3601639150963166	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5385	0.3009282673070077	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	5054	0.2824310980444971	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2942	0.16440686395862894	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2922	0.1632892102267552	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	2770	0.15479504186451465	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2661	0.14870382902580273	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2568	0.14350673917258977	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2485	0.13886847618531367	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2450	0.1369125821545346	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2447	0.13674493409475355	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2438	0.13624198991541037	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2365	0.13216255379407119	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2301	0.12858606185207516	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	2269	0.12679781588107716	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2209	0.12344485468545592	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	2202	0.1230536758793001	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2178	0.12171249140105159	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2177	0.12165660871445792	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	2122	0.1185830609518051	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2114	0.11813599945905559	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2102	0.11746540721993134	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2091	0.11685069766740079	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2057	0.1149506863232154	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2032	0.11355361915837321	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1993	0.11137419438121939	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1955	0.10925065229065926	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1932	0.10796535049900444	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1929	0.10779770243922338	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1916	0.10707122751350544	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1891	0.10567416034866325	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1854	0.10360650094469682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.58826865936876E-5
2	0.0	0.0	0.0	0.0	5.58826865936876E-5
3	0.0	0.0	0.0	0.0	5.58826865936876E-5
4	0.0	0.0	0.0	0.0	5.58826865936876E-5
5	0.0	0.0	0.0	0.0	5.58826865936876E-5
6	0.0	0.0	0.0	0.0	1.117653731873752E-4
7	0.0	0.0	0.0	0.0	1.117653731873752E-4
8	0.0	0.0	0.0	0.0	1.117653731873752E-4
9	0.0	0.0	0.0	5.58826865936876E-5	1.117653731873752E-4
10	0.0	0.0	0.0	5.58826865936876E-5	1.117653731873752E-4
11	0.0	0.0	0.0	1.117653731873752E-4	1.117653731873752E-4
12	0.0	0.0	0.0	1.117653731873752E-4	3.352961195621256E-4
13	0.0	0.0	0.0	1.676480597810628E-4	3.352961195621256E-4
14	0.0	0.0	0.0	2.235307463747504E-4	3.352961195621256E-4
15	0.0	0.0	0.0	2.79413432968438E-4	6.147095525305637E-4
16	0.0	0.0	0.0	2.79413432968438E-4	6.705922391242512E-4
17	0.0	0.0	0.0	2.79413432968438E-4	6.705922391242512E-4
18	0.0	0.0	0.0	4.470614927495008E-4	6.705922391242512E-4
19	0.0	0.0	0.0	5.58826865936876E-4	6.705922391242512E-4
20	0.0	0.0	0.0	5.58826865936876E-4	9.500056720926893E-4
21	0.0	0.0	0.0	6.705922391242512E-4	9.500056720926893E-4
22	0.0	0.0	0.0	0.0014529498514358778	0.0010617710452800644
23	0.0	0.0	0.0	0.003185313135840193	0.0010617710452800644
24	0.0	0.0	0.0	0.00653827433146145	0.001117653731873752
25	0.0	0.0	0.0	0.008214754929272077	0.001117653731873752
26	0.0	0.0	0.0	0.010561827766206957	0.001117653731873752
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	50	7.212138E-4	19.194433	4
CGTAAAC	190	0.0	16.8424	32
GTATTAG	465	0.0	16.53028	1
CATCGTT	470	0.0	16.33934	28
CCCACGA	70	3.6941952E-4	16.00207	2
GGTTCCG	260	0.0	15.997597	8
ATCGTTT	495	0.0	15.515423	29
GTGTTAC	95	1.4217096E-5	15.170881	1
TATTAGC	430	0.0	14.885645	2
AACGAAT	140	1.27947715E-8	14.857403	31
TAATACT	240	0.0	14.662415	4
TAGGACG	145	2.0396328E-8	14.340669	4
TTAGGCA	190	1.8189894E-11	14.311639	4
ATTAGCT	425	0.0	14.302533	3
GATATAC	225	0.0	14.234407	1
CGGTGTT	135	1.3182398E-7	14.223663	14
GTCGTAA	205	3.6379788E-12	14.0490265	30
TACCGTC	410	0.0	14.04667	7
TCTAGCG	505	0.0	13.93967	28
TATACAC	345	0.0	13.909787	3
>>END_MODULE