FastQCFastQC Report
Thu 2 Feb 2017
SRR4063067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789463
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT211951.1844335423532086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT146570.8190725374036792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT86500.48338523903539776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65080.36368452435171894No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA45180.2524779780302806No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA29960.16742452903468807No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC28940.16172449500213193No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28490.15920977410541598No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG28450.15898624335904124No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.15792447231376117No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25940.14495968902402565No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25630.14322732573962133No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT25470.1423332027541223No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25310.14143907976862333No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25300.14138319708202962No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25040.13993024723059377No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23910.13361550364550706No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA23410.13082136931582267No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA22890.12791546961295092No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22780.12730076006042035No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC22580.1261831063285466No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22400.12517721796986023No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21780.12171249140105159No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT21510.12020365886302203No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21350.11930953587752303No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21330.11919777050433565No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT20700.11567716124893333No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20310.11349773647177952No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT20270.11327420572540478No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA20080.11221243468012472No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19920.11131831169462572No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19730.11025654064934563No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA19360.1081888812453792No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC19090.10668004870734962No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18950.10589769109503801No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT18450.10310355676535361No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18230.10187413766029249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG5750.016.1403568
TACCGTC6100.016.0016617
ATACCGT5950.015.5982596
CTAGCGG5650.015.57466329
AACTTAC1255.135371E-815.3615967
TCTAGCG5850.015.31569128
ACACCGT1153.34383E-715.3059396
TGGATCG756.241838E-414.9348855
ATAGCGC650.004160682714.7703528
AACCGCG4450.014.7431057
CCGTCGT6300.014.7308669
CGTCGTA6200.014.70997310
AGAACCG5050.014.5757715
GTTCAAA7400.014.2725521
GTCCTAG1258.5818283E-714.0822511
ATAGTGC800.00101796514.0006723
GTCGTAG6550.013.92394411
CTCTTAC1155.6086956E-613.9152671
CGCGGTC4600.013.91332210
CGAAAAC5900.013.83001423
Produced by FastQC (version 0.11.5)