##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1789463
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.962227215650728	32.0	32.0	32.0	32.0	32.0
2	31.238362011396717	32.0	32.0	32.0	32.0	32.0
3	31.341395714803827	32.0	32.0	32.0	32.0	32.0
4	31.46408168260534	32.0	32.0	32.0	32.0	32.0
5	31.347162249233428	32.0	32.0	32.0	32.0	32.0
6	34.849319600349375	36.0	36.0	36.0	32.0	36.0
7	34.886813530092546	36.0	36.0	36.0	32.0	36.0
8	34.84843385976687	36.0	36.0	36.0	32.0	36.0
9	34.93266918623073	36.0	36.0	36.0	32.0	36.0
10	34.79126810668899	36.0	36.0	36.0	32.0	36.0
11	34.983442518789154	36.0	36.0	36.0	36.0	36.0
12	34.87154079184649	36.0	36.0	36.0	32.0	36.0
13	34.911249911286234	36.0	36.0	36.0	32.0	36.0
14	34.860121164841075	36.0	36.0	36.0	32.0	36.0
15	34.826917907774565	36.0	36.0	36.0	32.0	36.0
16	34.83139355214386	36.0	36.0	36.0	32.0	36.0
17	34.789446331106035	36.0	36.0	36.0	32.0	36.0
18	34.7779892627006	36.0	36.0	36.0	32.0	36.0
19	34.76187157823325	36.0	36.0	36.0	32.0	36.0
20	34.7406450985575	36.0	36.0	36.0	32.0	36.0
21	34.72281013913113	36.0	36.0	36.0	32.0	36.0
22	34.715445359864944	36.0	36.0	36.0	32.0	36.0
23	34.6661093300057	36.0	36.0	36.0	32.0	36.0
24	34.63880616698976	36.0	36.0	36.0	32.0	36.0
25	34.62181224199662	36.0	36.0	36.0	32.0	36.0
26	34.5516604702081	36.0	36.0	36.0	32.0	36.0
27	34.54437336787628	36.0	36.0	36.0	32.0	36.0
28	34.495131220930524	36.0	36.0	36.0	32.0	36.0
29	34.45030715918686	36.0	36.0	36.0	32.0	36.0
30	34.42203219625105	36.0	36.0	36.0	32.0	36.0
31	34.40961059267501	36.0	36.0	36.0	32.0	36.0
32	34.355048413965534	36.0	36.0	36.0	32.0	36.0
33	34.27638906196999	36.0	36.0	36.0	32.0	36.0
34	34.24415648716961	36.0	36.0	36.0	32.0	36.0
35	34.1890338051136	36.0	36.0	36.0	32.0	36.0
36	34.14312282511569	36.0	36.0	36.0	32.0	36.0
37	34.119872833358386	36.0	36.0	36.0	32.0	36.0
38	33.663540402902996	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	8.0
20	19.0
21	84.0
22	202.0
23	520.0
24	1387.0
25	3415.0
26	7135.0
27	13435.0
28	23705.0
29	37021.0
30	55210.0
31	79259.0
32	113426.0
33	178360.0
34	415148.0
35	861124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35632913656199	19.1194716181415	12.860585521138852	24.663613724157667
2	15.108666678215755	22.15988818992066	37.406864517455794	25.324580614407786
3	17.294629729701033	27.740892100032244	28.999426084808682	25.96505208545804
4	11.548400076000581	16.7444927520034	36.3878488482559	35.31925832374012
5	13.322000311825741	37.98098980642774	32.3086672467296	16.388342635016915
6	31.666068720249374	36.939956120791884	17.367605163127607	14.02636999583114
7	28.124795677785336	32.12160364088915	21.795766639302624	17.957834042022895
8	28.310163918302383	31.325317363984468	20.189300715088844	20.1752180026243
9	28.466053534414783	13.757473911515147	19.408502669343928	38.36796988472614
10	16.483956793404005	26.554431000827627	30.55856858731375	26.403043618454618
11	36.054022969877394	22.233088761682072	22.80529589183335	18.907592376607184
12	25.525117395947483	24.881737867967768	28.373858662152823	21.219286073931926
13	29.19292700454999	20.088306265486565	26.294612770152238	24.424153959811203
14	24.43856061846487	20.135314337317954	26.464643303605605	28.961481740611568
15	24.672485544546046	26.61971775890309	24.8543836894085	23.853413007142365
16	24.501889394689577	26.19826517690792	24.897036092412133	24.40280933599037
17	22.550396403837354	25.75459788774621	26.52678485109779	25.168220857318644
18	24.04760534305543	23.961769536447527	28.812051436660045	23.178573683836994
19	24.73982418189144	24.488407974906437	28.3176014256791	22.454166417523023
20	25.365207327561397	23.067925964381494	27.664779880891643	23.902086827165466
21	27.16281606584329	23.3160711521514	26.61063862246788	22.910474159537426
22	25.331188927174757	23.56244502537634	27.244948481722442	23.86141756572646
23	22.995746754462388	23.429174324948853	28.367096834402837	25.207982086185922
24	23.590652614778847	25.20035340211002	27.74357446898874	23.46541951412239
25	23.951191979703374	24.02227487621964	27.92278117420898	24.103751969868004
26	23.413307135500578	24.981935901369184	28.587882049399234	23.016874913731005
27	24.265351410284996	24.701572149379057	27.265807972344746	23.767268467991197
28	22.90575440788661	24.46873726922546	28.738565703789348	23.886942619098576
29	22.999134377184664	24.710094592623598	28.649712232105383	23.641058798086352
30	23.153370592183244	24.899481017489606	28.679944765552573	23.26720362477458
31	23.603617398068582	24.970675560209962	27.14350618034572	24.28220086137573
32	23.089608446779845	25.205326961216855	27.0658292459805	24.6392353460228
33	22.868368890555434	24.535461197018325	27.888198861893205	24.707971050533036
34	23.66235010167855	24.806268696251333	28.065738157201352	23.465643044868767
35	24.825604105812747	24.66332078394468	27.644159169538575	22.866915940704
36	23.40584857021352	25.734200707139514	27.05331152418351	23.80663919846345
37	24.332048217817302	25.78181275611734	27.012014218790775	22.874124807274583
38	23.12862748692065	25.497719426285663	27.620256814618028	23.75339627217566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2252.0
1	1583.0
2	914.0
3	914.0
4	3014.5
5	5115.0
6	5115.0
7	6024.0
8	6933.0
9	6573.0
10	6213.0
11	6213.0
12	7958.0
13	9703.0
14	11523.0
15	13343.0
16	13343.0
17	19560.5
18	25778.0
19	25778.0
20	28282.0
21	30786.0
22	26952.5
23	23119.0
24	23119.0
25	21967.0
26	20815.0
27	20815.0
28	26944.5
29	33074.0
30	39438.5
31	45803.0
32	45803.0
33	55076.0
34	64349.0
35	64349.0
36	68859.5
37	73370.0
38	83471.0
39	93572.0
40	93572.0
41	99506.0
42	105440.0
43	122101.5
44	138763.0
45	138763.0
46	142934.0
47	147105.0
48	147105.0
49	154101.5
50	161098.0
51	156942.0
52	152786.0
53	152786.0
54	143663.5
55	134541.0
56	134541.0
57	132001.5
58	129462.0
59	115781.0
60	102100.0
61	102100.0
62	99120.0
63	96140.0
64	79099.0
65	62058.0
66	62058.0
67	52153.0
68	42248.0
69	42248.0
70	33997.5
71	25747.0
72	19862.0
73	13977.0
74	13977.0
75	10241.0
76	6505.0
77	6505.0
78	6677.0
79	6849.0
80	5272.0
81	3695.0
82	3695.0
83	3704.5
84	3714.0
85	3714.0
86	2375.0
87	1036.0
88	887.0
89	738.0
90	738.0
91	459.0
92	180.0
93	125.5
94	71.0
95	71.0
96	57.0
97	43.0
98	43.0
99	35.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008438285675646829
2	0.0
3	0.0
4	1.676480597810628E-4
5	1.117653731873752E-4
6	2.79413432968438E-4
7	5.58826865936876E-4
8	0.001955894030779066
9	0.004023553434745508
10	7.823576123116264E-4
11	0.004247084181120258
12	3.352961195621256E-4
13	5.029441793431885E-4
14	0.0
15	0.0
16	5.58826865936876E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.117653731873752E-4
22	5.58826865936876E-5
23	3.352961195621256E-4
24	0.0
25	1.676480597810628E-4
26	1.117653731873752E-4
27	1.117653731873752E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.58826865936876E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1789463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.05669918880997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.11720625737478	22.517338047119168
2	16.463715669478912	11.213996241710808
3	6.926358956837938	7.076667704002479
4	3.4367282428122246	4.681744798365736
5	1.8964090278299983	3.22927158998749
6	1.1819844831697015	2.4152693993490923
7	0.7965702749877948	1.8989987966604873
8	0.5450194101693302	1.484924968335961
9	0.40253291788783674	1.2338048248290001
>10	1.8796976464921917	11.283476894217683
>50	0.1473818245157833	3.5567963024907256
>100	0.16376670851781858	12.05037109216486
>500	0.027815559874312536	6.445493502842102
>1k	0.014154663604679752	8.052550749915563
>5k	3.291782233646454E-4	0.8496607516965299
>10k+	3.291782233646454E-4	2.0096343363124416
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	21195	1.1844335423532086	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	14657	0.8190725374036792	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	8650	0.48338523903539776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6508	0.36368452435171894	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	4518	0.2524779780302806	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2996	0.16742452903468807	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2894	0.16172449500213193	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2849	0.15920977410541598	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2845	0.15898624335904124	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2826	0.15792447231376117	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2594	0.14495968902402565	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2563	0.14322732573962133	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	2547	0.1423332027541223	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2531	0.14143907976862333	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2530	0.14138319708202962	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2504	0.13993024723059377	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2391	0.13361550364550706	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	2341	0.13082136931582267	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	2289	0.12791546961295092	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2278	0.12730076006042035	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2258	0.1261831063285466	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2240	0.12517721796986023	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2178	0.12171249140105159	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2151	0.12020365886302203	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2135	0.11930953587752303	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2133	0.11919777050433565	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2070	0.11567716124893333	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2031	0.11349773647177952	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	2027	0.11327420572540478	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	2008	0.11221243468012472	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1992	0.11131831169462572	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1973	0.11025654064934563	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1936	0.1081888812453792	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1909	0.10668004870734962	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1895	0.10589769109503801	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1845	0.10310355676535361	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1823	0.10187413766029249	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.58826865936876E-5
6	0.0	0.0	0.0	0.0	1.117653731873752E-4
7	0.0	0.0	0.0	0.0	1.117653731873752E-4
8	0.0	0.0	0.0	0.0	1.117653731873752E-4
9	0.0	0.0	0.0	0.0	1.117653731873752E-4
10	0.0	0.0	0.0	0.0	1.676480597810628E-4
11	0.0	0.0	0.0	0.0	2.235307463747504E-4
12	0.0	0.0	0.0	0.0	6.147095525305637E-4
13	0.0	0.0	0.0	1.117653731873752E-4	7.264749257179389E-4
14	0.0	0.0	0.0	1.676480597810628E-4	7.264749257179389E-4
15	0.0	0.0	0.0	1.676480597810628E-4	8.382402989053141E-4
16	0.0	0.0	0.0	1.676480597810628E-4	8.382402989053141E-4
17	0.0	0.0	0.0	1.676480597810628E-4	8.382402989053141E-4
18	0.0	0.0	0.0	3.911788061558132E-4	0.001005888358686377
19	0.0	0.0	0.0	5.029441793431885E-4	0.0010617710452800644
20	0.0	0.0	0.0	5.58826865936876E-4	0.0011735364184674397
21	0.0	0.0	0.0	6.705922391242512E-4	0.0011735364184674397
22	0.0	0.0	0.0	0.0013970671648421901	0.0011735364184674397
23	0.0	0.0	0.0	0.003241195822433881	0.0011735364184674397
24	0.0	0.0	0.0	0.0067618050778362	0.0011735364184674397
25	0.0	0.0	0.0	0.00838240298905314	0.0011735364184674397
26	0.0	0.0	0.0	0.010673593139394331	0.0012853017916548148
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	575	0.0	16.140356	8
TACCGTC	610	0.0	16.001661	7
ATACCGT	595	0.0	15.598259	6
CTAGCGG	565	0.0	15.574663	29
AACTTAC	125	5.135371E-8	15.361596	7
TCTAGCG	585	0.0	15.315691	28
ACACCGT	115	3.34383E-7	15.305939	6
TGGATCG	75	6.241838E-4	14.934885	5
ATAGCGC	65	0.0041606827	14.770352	8
AACCGCG	445	0.0	14.743105	7
CCGTCGT	630	0.0	14.730866	9
CGTCGTA	620	0.0	14.709973	10
AGAACCG	505	0.0	14.575771	5
GTTCAAA	740	0.0	14.272552	1
GTCCTAG	125	8.5818283E-7	14.082251	1
ATAGTGC	80	0.001017965	14.000672	3
GTCGTAG	655	0.0	13.923944	11
CTCTTAC	115	5.6086956E-6	13.915267	1
CGCGGTC	460	0.0	13.913322	10
CGAAAAC	590	0.0	13.830014	23
>>END_MODULE