FastQCFastQC Report
Thu 2 Feb 2017
SRR4063066_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063066_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832576
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51220.6151990929356599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35610.42770870166807595No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21990.2641200322853409No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18360.22052040894765162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18320.22003997232685063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17930.21535571527404104No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA13330.1601055038819279No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10230.12287166576985165No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10220.1227515566146514No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10200.1225113383042509No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10180.12227111999385042No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9990.11998904604504573No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9640.11578522561303713No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9420.11314282419863171No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9370.11254227842263048No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9320.11194173264662925No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9090.10917922207702359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8350.10029114459220541No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGAC453.542323E-421.327373
GTAATAG400.004452395720.0232681
CTAGCGG1400.019.42780529
GTATTAG2650.018.1342811
CGCAATA1302.582965E-1017.234232
TATACAC2150.017.1114923
TAGTACA753.239727E-517.0618954
TCTATAT753.239727E-517.0618953
GTTCTAA1051.1722659E-716.7814061
GATATAC2100.016.7814061
CCAACGA1252.8521754E-916.63934329
TCTAGCG1651.8189894E-1216.48419828
TAGCGGC1651.8189894E-1216.48320830
CAAGACG2050.016.385664
GTTTAAG600.00242227316.0186141
GTCCTAT2600.016.0186141
TCTAGGC600.002445707315.9955263
AAGACGG2200.015.9955245
ATACACT2400.015.3290464
CGTGATT951.4319827E-515.15729728
Produced by FastQC (version 0.11.5)