##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063066_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832576
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.105007831116918	32.0	32.0	32.0	32.0	32.0
2	30.779988853870396	32.0	32.0	32.0	32.0	32.0
3	30.85890777538627	32.0	32.0	32.0	32.0	32.0
4	30.882795084172496	32.0	32.0	32.0	32.0	32.0
5	30.855435419709433	32.0	32.0	32.0	32.0	32.0
6	34.46414261280652	36.0	36.0	36.0	32.0	36.0
7	34.34707942578215	36.0	36.0	36.0	32.0	36.0
8	34.33181235106465	36.0	36.0	36.0	32.0	36.0
9	34.41784293565993	36.0	36.0	36.0	32.0	36.0
10	34.20347211545853	36.0	36.0	36.0	32.0	36.0
11	34.47088073641325	36.0	36.0	36.0	32.0	36.0
12	34.302802386809134	36.0	36.0	36.0	32.0	36.0
13	34.363310977015914	36.0	36.0	36.0	32.0	36.0
14	34.28269851641171	36.0	36.0	36.0	32.0	36.0
15	34.2499675705281	36.0	36.0	36.0	32.0	36.0
16	34.248533467215005	36.0	36.0	36.0	32.0	36.0
17	34.165852726958256	36.0	36.0	36.0	32.0	36.0
18	34.199317539780154	36.0	36.0	36.0	32.0	36.0
19	34.171848575985855	36.0	36.0	36.0	32.0	36.0
20	34.14314609116765	36.0	36.0	36.0	32.0	36.0
21	34.11094602775002	36.0	36.0	36.0	32.0	36.0
22	34.07376263548313	36.0	36.0	36.0	32.0	36.0
23	34.0366753305404	36.0	36.0	36.0	32.0	36.0
24	34.034676714197865	36.0	36.0	36.0	32.0	36.0
25	33.98665347067415	36.0	36.0	36.0	32.0	36.0
26	33.94870498308863	36.0	36.0	36.0	32.0	36.0
27	33.9409783611346	36.0	36.0	36.0	32.0	36.0
28	33.93062134868168	36.0	36.0	36.0	32.0	36.0
29	33.902472567068955	36.0	36.0	36.0	32.0	36.0
30	33.85357492889538	36.0	36.0	36.0	32.0	36.0
31	33.876879708278885	36.0	36.0	36.0	32.0	36.0
32	33.812717397570914	36.0	36.0	36.0	32.0	36.0
33	33.76513735682989	36.0	36.0	36.0	32.0	36.0
34	33.74419512452917	36.0	36.0	36.0	27.0	36.0
35	33.686380582673536	36.0	36.0	36.0	27.0	36.0
36	33.63908279844723	36.0	36.0	36.0	27.0	36.0
37	33.61200298831578	36.0	36.0	36.0	27.0	36.0
38	32.991922659312785	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	0.0
10	8.0
11	6.0
12	7.0
13	1.0
14	246.0
15	438.0
16	537.0
17	617.0
18	741.0
19	991.0
20	1273.0
21	1537.0
22	2220.0
23	3202.0
24	4468.0
25	6305.0
26	8986.0
27	12347.0
28	16971.0
29	23159.0
30	30457.0
31	40520.0
32	53754.0
33	80255.0
34	173843.0
35	369683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.36332697166593	17.264651377018385	11.495617183958014	24.87640446735767
2	16.216281177067234	20.520080470903807	37.683000401393095	25.58063795063586
3	19.078490585982642	25.474905163114634	28.31100252017461	27.13560173072811
4	12.297445814390642	15.659265754214434	36.19064205968374	35.85264637171118
5	14.049648320393574	37.29617476362518	33.23744619109847	15.416730724882774
6	32.98913970409838	35.82840684431654	17.44889943716715	13.733554014417937
7	29.289578368821584	31.086411330617263	21.287065685294795	18.336944615266354
8	28.563621448209346	31.910475117497207	20.039083659931322	19.486819774362125
9	28.074587874966216	14.25685373690058	18.896555865837914	38.77200252229529
10	16.057609501171672	26.60277621968577	31.30195502846004	26.037659250682527
11	36.869281964247755	21.760275198393394	22.322154599711975	19.048288237646883
12	25.115337171299572	24.23660064932335	28.800000960892863	21.848061218484215
13	29.754521265413747	19.69199536311272	25.64907412412683	24.904409247346702
14	24.196156156156157	20.204564564564564	25.555315315315312	30.043963963963964
15	25.174138796850485	26.789382759358084	23.872813203553175	24.163665240238256
16	25.271507045789527	25.832479462043068	24.605787745815828	24.290225746351577
17	23.234972231198846	25.647247927401075	26.270499237754517	24.847280603645565
18	24.509208054465173	24.41455703804779	27.710526442226847	23.365708465260195
19	24.943244743081863	25.31933090056479	26.677733441278427	23.059690915074917
20	25.227229321741344	23.85790765246616	26.723273303392435	24.19158972240006
21	26.75532055455325	24.25774089938332	25.178913331235304	23.808025214828128
22	25.19963484360736	24.502714649497907	25.90352183731322	24.394128669581512
23	23.59438944964763	24.319552001614408	26.591255527007107	25.494803021730856
24	23.908449985164506	25.772797109300953	26.20176512349498	24.116987782039562
25	24.612850794840654	24.54533769165252	26.236408783430697	24.605402730076126
26	23.634463371855556	25.45109439946181	27.12933374978977	23.785108478892866
27	24.633666743943294	25.250358255245903	26.000622216990365	24.115352783820438
28	23.637798472369198	24.807604761864724	26.995511278258704	24.559085487507375
29	23.356316592391497	25.36558997705678	27.240086968011628	24.03800646254009
30	23.90637461684075	25.36033785277076	27.132119074844574	23.601168455543917
31	23.968395436604695	25.390192399626205	25.972147817877385	24.66926434589172
32	23.418459701607276	25.680728673064056	26.033029559401676	24.867782065926992
33	23.386089329643298	25.106424892373923	26.519978090405903	24.987507687576876
34	24.040159426870492	25.42265178105953	26.819078370463266	23.718110421606713
35	25.12126737925517	25.177846810289527	26.55197018948704	23.148915620968268
36	23.721791234110235	26.297308920372775	25.83794419871849	24.1429556467985
37	24.730750103904153	26.069972540474573	25.921623835732937	23.277653519888336
38	23.627645403343305	25.941318560674766	26.234521560209917	24.196514475772016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1002.0
1	741.0
2	480.0
3	480.0
4	1110.0
5	1740.0
6	1740.0
7	1957.5
8	2175.0
9	2017.5
10	1860.0
11	1860.0
12	2337.5
13	2815.0
14	3461.5
15	4108.0
16	4108.0
17	5702.0
18	7296.0
19	7296.0
20	8227.5
21	9159.0
22	8728.5
23	8298.0
24	8298.0
25	9054.0
26	9810.0
27	9810.0
28	12582.0
29	15354.0
30	18351.0
31	21348.0
32	21348.0
33	25646.5
34	29945.0
35	29945.0
36	32602.5
37	35260.0
38	40070.0
39	44880.0
40	44880.0
41	47649.5
42	50419.0
43	57346.0
44	64273.0
45	64273.0
46	68474.0
47	72675.0
48	72675.0
49	74303.0
50	75931.0
51	74402.5
52	72874.0
53	72874.0
54	69297.0
55	65720.0
56	65720.0
57	63749.0
58	61778.0
59	55418.5
60	49059.0
61	49059.0
62	47149.5
63	45240.0
64	37184.0
65	29128.0
66	29128.0
67	24202.0
68	19276.0
69	19276.0
70	15603.5
71	11931.0
72	9205.0
73	6479.0
74	6479.0
75	4906.0
76	3333.0
77	3333.0
78	3240.0
79	3147.0
80	2484.0
81	1821.0
82	1821.0
83	1886.0
84	1951.0
85	1951.0
86	1294.0
87	637.0
88	548.0
89	459.0
90	459.0
91	339.5
92	220.0
93	182.0
94	144.0
95	144.0
96	131.0
97	118.0
98	118.0
99	275.5
100	433.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1240727573218541
2	0.056931739564916595
3	0.011290260588823122
4	0.0025222922592051658
5	0.0
6	2.4021831040049198E-4
7	0.0
8	3.6032746560073794E-4
9	1.2010915520024599E-4
10	6.005457760012299E-4
11	0.0015614190176031979
12	0.002041855638404182
13	0.014533207779229763
14	0.009128295795218695
15	0.023301176108847722
16	0.011170151433622877
17	0.02005822891844108
18	0.005284802828810823
19	0.006485894380813283
20	0.005645130294411561
21	0.006005457760012299
22	0.006726112691213775
23	0.008767968329617957
24	0.013572334537627795
25	0.017415827504035666
26	0.018737028211238373
27	0.008287531708816973
28	0.005404911984011069
29	0.010329387347221154
30	0.0028826197248059036
31	0.004564147897609347
32	0.005885348604812053
33	0.007686985932815743
34	0.011290260588823122
35	0.014172880313629026
36	0.01465331693443001
37	0.009368514105619187
38	0.005645130294411561
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	832576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.49516437574695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41288809800643	40.37884561783893
2	12.930789320911071	13.31746243176938
3	4.013851373547479	6.200818087819353
4	1.624371458795685	3.3458910111182267
5	0.8097416642245798	2.084889005056782
6	0.44039684090257986	1.3606984627702818
7	0.3118956426510056	1.1242782170474936
8	0.198698014894871	0.8185589550516799
9	0.15447910497763181	0.7159434213097278
>10	0.8655506863123015	8.506900394291037
>50	0.10851766800516764	4.000257535590065
>100	0.11598478805442888	11.813119875824363
>500	0.010268270973425492	3.71746744878666
>1k	0.002333697948505794	1.9993374909634594
>5k	2.3336979485057938E-4	0.6155320447625677
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5122	0.6151990929356599	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3561	0.42770870166807595	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2199	0.2641200322853409	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1836	0.22052040894765162	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1832	0.22003997232685063	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1793	0.21535571527404104	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1333	0.1601055038819279	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1023	0.12287166576985165	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1022	0.1227515566146514	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1020	0.1225113383042509	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1018	0.12227111999385042	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	999	0.11998904604504573	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	964	0.11578522561303713	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	942	0.11314282419863171	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	937	0.11254227842263048	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	932	0.11194173264662925	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	909	0.10917922207702359	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	835	0.10029114459220541	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2010915520024598E-4
2	0.0	0.0	0.0	0.0	1.2010915520024598E-4
3	0.0	0.0	0.0	0.0	1.2010915520024598E-4
4	0.0	0.0	0.0	0.0	1.2010915520024598E-4
5	0.0	0.0	0.0	1.2010915520024598E-4	1.2010915520024598E-4
6	0.0	0.0	0.0	1.2010915520024598E-4	1.2010915520024598E-4
7	0.0	0.0	0.0	1.2010915520024598E-4	1.2010915520024598E-4
8	0.0	0.0	0.0	1.2010915520024598E-4	1.2010915520024598E-4
9	0.0	0.0	0.0	1.2010915520024598E-4	1.2010915520024598E-4
10	0.0	0.0	0.0	2.4021831040049195E-4	1.2010915520024598E-4
11	0.0	0.0	0.0	2.4021831040049195E-4	1.2010915520024598E-4
12	0.0	0.0	0.0	2.4021831040049195E-4	3.6032746560073794E-4
13	0.0	0.0	0.0	2.4021831040049195E-4	3.6032746560073794E-4
14	0.0	0.0	0.0	2.4021831040049195E-4	3.6032746560073794E-4
15	0.0	0.0	0.0	3.6032746560073794E-4	6.005457760012299E-4
16	0.0	0.0	0.0	4.804366208009839E-4	7.206549312014759E-4
17	0.0	0.0	0.0	4.804366208009839E-4	7.206549312014759E-4
18	0.0	0.0	0.0	7.206549312014759E-4	7.206549312014759E-4
19	0.0	0.0	0.0	8.407640864017219E-4	7.206549312014759E-4
20	0.0	0.0	0.0	0.001321200707202706	7.206549312014759E-4
21	0.0	0.0	0.0	0.0018016373280036898	7.206549312014759E-4
22	0.0	0.0	0.0	0.002642401414405412	7.206549312014759E-4
23	0.0	0.0	0.0	0.004203820432008609	8.407640864017219E-4
24	0.0	0.0	0.0	0.007086440156814513	8.407640864017219E-4
25	0.0	0.0	0.0	0.009728841571219924	8.407640864017219E-4
26	0.0	0.0	0.0	0.012010915520024599	8.407640864017219E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGAC	45	3.542323E-4	21.32737	3
GTAATAG	40	0.0044523957	20.023268	1
CTAGCGG	140	0.0	19.427805	29
GTATTAG	265	0.0	18.134281	1
CGCAATA	130	2.582965E-10	17.23423	2
TATACAC	215	0.0	17.111492	3
TAGTACA	75	3.239727E-5	17.061895	4
TCTATAT	75	3.239727E-5	17.061895	3
GTTCTAA	105	1.1722659E-7	16.781406	1
GATATAC	210	0.0	16.781406	1
CCAACGA	125	2.8521754E-9	16.639343	29
TCTAGCG	165	1.8189894E-12	16.484198	28
TAGCGGC	165	1.8189894E-12	16.483208	30
CAAGACG	205	0.0	16.38566	4
GTTTAAG	60	0.002422273	16.018614	1
GTCCTAT	260	0.0	16.018614	1
TCTAGGC	60	0.0024457073	15.995526	3
AAGACGG	220	0.0	15.995524	5
ATACACT	240	0.0	15.329046	4
CGTGATT	95	1.4319827E-5	15.157297	28
>>END_MODULE