FastQCFastQC Report
Thu 2 Feb 2017
SRR4063066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832576
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT59040.7091244523022523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT43440.5217541701898686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23640.2839380428933815No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23210.2787733492197709No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21530.2585950111461296No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.2332519793988777No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12270.14737393343070182No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11470.13776520101468215No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11140.13380159889307403No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10680.12827657775386272No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10400.12491352140825583No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9980.1198689368898455No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9790.11758686294104082No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9770.11734664463064033No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9700.1165058805442386No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9670.11614555307863786No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9570.1149444615266354No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.10881889461142286No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8790.10557594742101621No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.10509551080021523No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8750.10509551080021523No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8720.10473518333461448No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8630.10365420093781227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTA200.003753685931.9986831
TCTAGCG2400.018.66589728
CTAGCGG2400.017.99925829
CACGTTT450.008850384517.77704428
GATAGGA550.001364595317.4538232
ATTATAC1201.6570993E-917.3326193
TATACTG854.9740192E-616.943535
GTATTAG2650.016.9059771
GATATAC1800.016.8892081
CATCGTT1800.016.88819328
CGTTCCA1051.1884549E-716.76121318
AACGAAT1252.8521754E-916.63931331
TAGCGGC2600.016.61469830
AGCGGCG2900.016.5510431
ATCGTTT1750.016.45646329
CCGATAA1407.403287E-1016.0022229
CACCGTG703.6909638E-416.0022227
GTCCTAA908.573579E-616.0003011
ATAGGGG600.002441819515.999343
ACGTTTT600.002441819515.9993429
Produced by FastQC (version 0.11.5)