##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832576
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10726828541779	32.0	32.0	32.0	32.0	32.0
2	31.297236528557153	32.0	32.0	32.0	32.0	32.0
3	31.37109044699823	32.0	32.0	32.0	32.0	32.0
4	31.48808757398724	32.0	32.0	32.0	32.0	32.0
5	31.398034533784305	32.0	32.0	32.0	32.0	32.0
6	34.92351689215159	36.0	36.0	36.0	36.0	36.0
7	34.94383816011992	36.0	36.0	36.0	36.0	36.0
8	34.897335498501036	36.0	36.0	36.0	36.0	36.0
9	34.994029373894996	36.0	36.0	36.0	36.0	36.0
10	34.835713496425555	36.0	36.0	36.0	32.0	36.0
11	35.02395096663848	36.0	36.0	36.0	36.0	36.0
12	34.91016075409332	36.0	36.0	36.0	32.0	36.0
13	34.960908073256974	36.0	36.0	36.0	36.0	36.0
14	34.91536988815435	36.0	36.0	36.0	32.0	36.0
15	34.874625259435774	36.0	36.0	36.0	32.0	36.0
16	34.87778413021754	36.0	36.0	36.0	32.0	36.0
17	34.84579305672995	36.0	36.0	36.0	32.0	36.0
18	34.84345092820355	36.0	36.0	36.0	32.0	36.0
19	34.82705242524406	36.0	36.0	36.0	32.0	36.0
20	34.81257326658467	36.0	36.0	36.0	32.0	36.0
21	34.78726866976709	36.0	36.0	36.0	32.0	36.0
22	34.76665193327696	36.0	36.0	36.0	32.0	36.0
23	34.711574679068335	36.0	36.0	36.0	32.0	36.0
24	34.68952504035668	36.0	36.0	36.0	32.0	36.0
25	34.669708230840186	36.0	36.0	36.0	32.0	36.0
26	34.60154027980629	36.0	36.0	36.0	32.0	36.0
27	34.580942760781	36.0	36.0	36.0	32.0	36.0
28	34.55111124990391	36.0	36.0	36.0	32.0	36.0
29	34.48932349719425	36.0	36.0	36.0	32.0	36.0
30	34.45497828426474	36.0	36.0	36.0	32.0	36.0
31	34.45478010415866	36.0	36.0	36.0	32.0	36.0
32	34.40256505111846	36.0	36.0	36.0	32.0	36.0
33	34.3476487431778	36.0	36.0	36.0	32.0	36.0
34	34.31923812360673	36.0	36.0	36.0	32.0	36.0
35	34.26080381851026	36.0	36.0	36.0	32.0	36.0
36	34.22327571296795	36.0	36.0	36.0	32.0	36.0
37	34.19971510108387	36.0	36.0	36.0	32.0	36.0
38	33.75593339226689	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	3.0
20	7.0
21	26.0
22	67.0
23	224.0
24	588.0
25	1460.0
26	3141.0
27	5788.0
28	10556.0
29	16511.0
30	24599.0
31	35338.0
32	50293.0
33	78785.0
34	187798.0
35	417391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.74572073100125	18.36031625149249	12.166217018097255	25.727745999409013
2	15.34706741486663	21.47575716811438	36.935607079714046	26.241568337304944
3	18.087598009070643	26.048913252363747	28.805178145898992	27.05831059266662
4	11.821998018196558	16.401645497402637	36.20478635558358	35.57157012881723
5	13.583384579906218	37.663108232761935	33.06232704281651	15.691180144515334
6	32.90302123294746	36.32565994133854	16.994405302111286	13.776913523602708
7	28.858439700638144	31.34363315561075	21.39745439127714	18.400472752473963
8	28.281599262034284	32.28323758436339	19.846521387673427	19.5886417659289
9	27.814746127531997	14.015235230810625	18.790958796171733	39.37905984548564
10	15.978812465092815	26.693771657468186	31.40343396611676	25.92398191132224
11	37.00521540261419	21.58530462419553	22.331820682398558	19.077659290791726
12	24.82229722425742	24.432780426871016	28.781363729175922	21.963558619695643
13	29.722487463591868	19.645677566585594	25.820136324054886	24.81169864576765
14	24.050417018986856	20.080449112153126	25.719694057959874	30.149439810900148
15	25.13656410946268	26.80307863786609	23.84190752555923	24.218449727112
16	25.23925743715889	25.754405603812746	24.5118763932662	24.494460565762164
17	23.277754823583674	25.652072603582138	26.16565935121839	24.904513221615804
18	24.553314051810286	24.178573487585517	27.85727669305865	23.410835767545546
19	25.014292989468828	25.141008148205092	26.729812053193942	23.11488680913214
20	25.281655968944577	23.699818394957337	26.89111864862787	24.127406987470213
21	26.73665827504036	24.166322353755092	25.426507610116072	23.670511761088477
22	25.144371204550698	24.28847336459374	26.130347259589513	24.436808171266048
23	23.644773491333492	23.903609653447806	26.758014011984532	25.693602843234164
24	23.943159543393037	25.475872472903376	26.437586478591747	24.143381505111847
25	24.532895495426242	24.234784572219233	26.53835805980475	24.693961872549774
26	23.665707394880467	25.255352063955723	27.20952801906373	23.869412522100085
27	24.710536935967404	24.92216926743024	26.263428203551385	24.103865593050966
28	23.66186390191406	24.47284091782612	27.375158544084865	24.490136636174956
29	23.556287954493044	24.95567972173111	27.351977477131218	24.13605484664463
30	23.93090840956261	25.051646936736105	27.412032054731338	23.605412598969945
31	23.976790106849105	25.05428933815051	26.141277192712735	24.827643362287645
32	23.508364401568148	25.407170036128836	26.233761242216925	24.850704320086095
33	23.433656507033593	24.708134752863405	26.800556345606886	25.057652394496117
34	24.066031209162887	24.989070066876778	27.136021216081176	23.80887750787916
35	25.262918940733336	24.788367668537166	26.687053193942656	23.261660196786842
36	23.787978514874318	25.72714082558229	26.234602006303327	24.250278653240066
37	24.830405872857252	25.591657698516414	26.165419132907985	23.41251729571835
38	23.865569029133678	25.28634022599739	26.502925859020678	24.34516488584826
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	616.0
1	459.0
2	302.0
3	302.0
4	1036.5
5	1771.0
6	1771.0
7	2000.0
8	2229.0
9	2039.0
10	1849.0
11	1849.0
12	2398.0
13	2947.0
14	3566.0
15	4185.0
16	4185.0
17	6082.0
18	7979.0
19	7979.0
20	8700.0
21	9421.0
22	8907.5
23	8394.0
24	8394.0
25	8888.5
26	9383.0
27	9383.0
28	12232.5
29	15082.0
30	18141.0
31	21200.0
32	21200.0
33	25822.5
34	30445.0
35	30445.0
36	32631.5
37	34818.0
38	39749.0
39	44680.0
40	44680.0
41	47167.0
42	49654.0
43	56505.5
44	63357.0
45	63357.0
46	68275.0
47	73193.0
48	73193.0
49	75349.5
50	77506.0
51	75277.5
52	73049.0
53	73049.0
54	69350.0
55	65651.0
56	65651.0
57	64029.0
58	62407.0
59	55918.0
60	49429.0
61	49429.0
62	47288.0
63	45147.0
64	37229.0
65	29311.0
66	29311.0
67	24267.0
68	19223.0
69	19223.0
70	15695.5
71	12168.0
72	9407.0
73	6646.0
74	6646.0
75	4896.0
76	3146.0
77	3146.0
78	3200.0
79	3254.0
80	2465.5
81	1677.0
82	1677.0
83	1652.5
84	1628.0
85	1628.0
86	1045.5
87	463.0
88	371.0
89	279.0
90	279.0
91	163.5
92	48.0
93	33.5
94	19.0
95	19.0
96	14.5
97	10.0
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008888077484818202
2	0.0
3	0.0
4	1.2010915520024599E-4
5	0.0
6	2.4021831040049198E-4
7	6.005457760012299E-4
8	0.002041855638404182
9	0.0038434929664078717
10	0.0013212007072027057
11	0.0050445845184103316
12	7.206549312014759E-4
13	1.2010915520024599E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	3.6032746560073794E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	832576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.903811378071254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29740992127465	37.31234143425397
2	14.248283728151941	13.935907598055689
3	4.378663641047146	6.424000223693649
4	1.7624618403101393	3.447644055983016
5	0.891331038475341	2.1794742490509225
6	0.5150685027391652	1.5113287742845027
7	0.3280500959986917	1.1230030012094732
8	0.2353440077083573	0.9207375169543083
9	0.15576710085674123	0.6855844427286367
>10	0.9285344626246145	8.72805217787759
>50	0.11796797159741347	4.09411856338066
>100	0.12634096783261894	12.521509864053423
>500	0.012067655796262882	4.150591765724849
>1k	0.0024627868971965066	2.254630545365016
>5k	2.462786897196507E-4	0.7110757873843511
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5904	0.7091244523022523	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4344	0.5217541701898686	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2364	0.2839380428933815	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2321	0.2787733492197709	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2153	0.2585950111461296	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1942	0.2332519793988777	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1227	0.14737393343070182	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1147	0.13776520101468215	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1114	0.13380159889307403	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1068	0.12827657775386272	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1040	0.12491352140825583	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	998	0.1198689368898455	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	979	0.11758686294104082	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	977	0.11734664463064033	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	970	0.1165058805442386	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	967	0.11614555307863786	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	957	0.1149444615266354	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.10881889461142286	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	879	0.10557594742101621	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	875	0.10509551080021523	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	875	0.10509551080021523	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	872	0.10473518333461448	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	863	0.10365420093781227	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.2010915520024598E-4	0.0
6	0.0	0.0	0.0	1.2010915520024598E-4	2.4021831040049195E-4
7	0.0	0.0	0.0	1.2010915520024598E-4	2.4021831040049195E-4
8	0.0	0.0	0.0	1.2010915520024598E-4	2.4021831040049195E-4
9	0.0	0.0	0.0	1.2010915520024598E-4	3.6032746560073794E-4
10	0.0	0.0	0.0	1.2010915520024598E-4	3.6032746560073794E-4
11	0.0	0.0	0.0	1.2010915520024598E-4	3.6032746560073794E-4
12	0.0	0.0	0.0	1.2010915520024598E-4	6.005457760012299E-4
13	1.2010915520024598E-4	0.0	0.0	1.2010915520024598E-4	6.005457760012299E-4
14	1.2010915520024598E-4	0.0	0.0	1.2010915520024598E-4	7.206549312014759E-4
15	1.2010915520024598E-4	0.0	0.0	2.4021831040049195E-4	7.206549312014759E-4
16	1.2010915520024598E-4	0.0	0.0	3.6032746560073794E-4	7.206549312014759E-4
17	1.2010915520024598E-4	0.0	0.0	3.6032746560073794E-4	7.206549312014759E-4
18	1.2010915520024598E-4	0.0	0.0	4.804366208009839E-4	7.206549312014759E-4
19	1.2010915520024598E-4	0.0	0.0	7.206549312014759E-4	9.608732416019678E-4
20	1.2010915520024598E-4	0.0	0.0	0.0010809823968022138	0.0010809823968022138
21	1.2010915520024598E-4	0.0	0.0	0.0015614190176031979	0.001321200707202706
22	1.2010915520024598E-4	0.0	0.0	0.002642401414405412	0.001321200707202706
23	1.2010915520024598E-4	0.0	0.0	0.004564147897609347	0.001321200707202706
24	1.2010915520024598E-4	0.0	0.0	0.007446767622415251	0.001321200707202706
25	1.2010915520024598E-4	0.0	0.0	0.010209278192020908	0.001321200707202706
26	1.2010915520024598E-4	0.0	0.0	0.01225113383042509	0.001321200707202706
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGTA	20	0.0037536859	31.99868	31
TCTAGCG	240	0.0	18.665897	28
CTAGCGG	240	0.0	17.999258	29
CACGTTT	45	0.0088503845	17.777044	28
GATAGGA	55	0.0013645953	17.453823	2
ATTATAC	120	1.6570993E-9	17.332619	3
TATACTG	85	4.9740192E-6	16.94353	5
GTATTAG	265	0.0	16.905977	1
GATATAC	180	0.0	16.889208	1
CATCGTT	180	0.0	16.888193	28
CGTTCCA	105	1.1884549E-7	16.761213	18
AACGAAT	125	2.8521754E-9	16.639313	31
TAGCGGC	260	0.0	16.614698	30
AGCGGCG	290	0.0	16.55104	31
ATCGTTT	175	0.0	16.456463	29
CCGATAA	140	7.403287E-10	16.002222	9
CACCGTG	70	3.6909638E-4	16.002222	7
GTCCTAA	90	8.573579E-6	16.000301	1
ATAGGGG	60	0.0024418195	15.99934	3
ACGTTTT	60	0.0024418195	15.99934	29
>>END_MODULE