FastQCFastQC Report
Thu 2 Feb 2017
SRR4063065_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063065_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279067
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT63580.278973808141665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38870.17055224791548473No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32000.14040833376113998No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC29150.12790321653553843No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA26360.11566136493573904No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24840.1089919690820849No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24180.10609604719826139No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23910.10491135188215178No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23780.10434094302624713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23000.10091848989081935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA400.004483721720.00083714
TCTAGCG6200.019.87049128
CTAGCGG6400.018.99954429
TAGCGGC6800.017.88192430
ACGAACG2950.016.81426215
AGCGGCG7550.016.7413231
TAGCGTA3250.016.7365917
CGAACGA2900.016.55241616
ACCGTCG5900.016.5405248
AAGACGG7600.016.4166955
CAAGACG7650.016.3093954
ATACCGT6300.016.2500116
AATAGCG3350.016.2344975
ATAGCGT3450.016.2279326
GACGGAC7200.016.220417
TACCGTC6250.016.1261967
AGCGTAT3500.015.9982118
CGCAAGA7050.015.8913752
CGTCGTA5950.015.59660610
ATAACGA3200.015.49996912
Produced by FastQC (version 0.11.5)