##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063065_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2279067
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21816208123763	32.0	32.0	32.0	32.0	32.0
2	30.834501135771788	32.0	32.0	32.0	32.0	32.0
3	30.878211127623715	32.0	32.0	32.0	32.0	32.0
4	30.94906161161563	32.0	32.0	32.0	32.0	32.0
5	30.858632501808856	32.0	32.0	32.0	32.0	32.0
6	34.499416647250825	36.0	36.0	36.0	32.0	36.0
7	34.43228479022337	36.0	36.0	36.0	32.0	36.0
8	34.41571441295934	36.0	36.0	36.0	32.0	36.0
9	34.55347253942074	36.0	36.0	36.0	32.0	36.0
10	34.27739553071498	36.0	36.0	36.0	32.0	36.0
11	34.54957489183074	36.0	36.0	36.0	32.0	36.0
12	34.3726134422551	36.0	36.0	36.0	32.0	36.0
13	34.44523526513262	36.0	36.0	36.0	32.0	36.0
14	34.347896748976666	36.0	36.0	36.0	32.0	36.0
15	34.291358700731486	36.0	36.0	36.0	32.0	36.0
16	34.30891500776414	36.0	36.0	36.0	32.0	36.0
17	34.231348617658014	36.0	36.0	36.0	32.0	36.0
18	34.25868831412152	36.0	36.0	36.0	32.0	36.0
19	34.249846099302914	36.0	36.0	36.0	32.0	36.0
20	34.23185189377934	36.0	36.0	36.0	32.0	36.0
21	34.21819586699294	36.0	36.0	36.0	32.0	36.0
22	34.1950065531202	36.0	36.0	36.0	32.0	36.0
23	34.13294914103008	36.0	36.0	36.0	32.0	36.0
24	34.120286941981085	36.0	36.0	36.0	32.0	36.0
25	34.095978749198686	36.0	36.0	36.0	32.0	36.0
26	34.04460421742757	36.0	36.0	36.0	32.0	36.0
27	34.05139164403679	36.0	36.0	36.0	32.0	36.0
28	34.0338910615616	36.0	36.0	36.0	32.0	36.0
29	34.00273840128438	36.0	36.0	36.0	32.0	36.0
30	33.97688176784623	36.0	36.0	36.0	32.0	36.0
31	33.99389750279391	36.0	36.0	36.0	32.0	36.0
32	33.95168285969653	36.0	36.0	36.0	32.0	36.0
33	33.905370048357504	36.0	36.0	36.0	32.0	36.0
34	33.92445154091565	36.0	36.0	36.0	32.0	36.0
35	33.87890834275605	36.0	36.0	36.0	32.0	36.0
36	33.836655964918975	36.0	36.0	36.0	32.0	36.0
37	33.83533481025349	36.0	36.0	36.0	32.0	36.0
38	33.24150891570981	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	5.0
8	4.0
9	7.0
10	27.0
11	13.0
12	7.0
13	12.0
14	150.0
15	451.0
16	687.0
17	804.0
18	1069.0
19	1419.0
20	1929.0
21	2759.0
22	4254.0
23	6549.0
24	10067.0
25	15194.0
26	22330.0
27	32367.0
28	45289.0
29	61394.0
30	82872.0
31	112254.0
32	152142.0
33	227567.0
34	496664.0
35	1000779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.707409948873575	17.227777489788334	11.54100613358942	25.523806427748667
2	16.607868944002853	19.38331754370099	38.046662973151186	25.962150539144975
3	19.47341675721124	23.336276397619102	28.1119394208541	29.07836742431556
4	12.464732307794916	15.21971498000437	36.13017650739492	36.1853762048058
5	14.692654528350236	36.39858292702618	33.591377863895	15.31738468072858
6	34.52746704998293	35.34469065728033	16.802643899365442	13.325198393371295
7	30.585876225557413	30.39584215456777	20.418812100576332	18.599469519298484
8	28.038921360896197	33.14945611276945	19.25190989080133	19.55971263553303
9	27.20279413442384	14.508964222091564	18.439707598746853	39.84853404473775
10	15.645752863140963	26.7349640398885	31.59075282584477	26.02853027112577
11	37.8534561399121	21.23345404252948	21.97039004734907	18.942699770209355
12	25.033007738883228	23.539201873093198	28.66010738868746	22.767682999336117
13	29.473755349513237	19.471964864361972	25.22955950554524	25.824720280579555
14	23.456756534976147	19.81773286824556	25.27284559381408	31.45266500296421
15	25.11678695005679	27.255993834561913	22.46026785949402	25.166951355887274
16	25.715043000437948	25.97715302784694	23.843894236212346	24.463909735502764
17	23.921312361783375	25.98268482242661	25.24788475624501	24.848118059545005
18	24.80912211971861	24.99741108162377	26.383798812081384	23.809667986576237
19	25.255049092205585	25.073687674839558	25.725193351982888	23.946069880971972
20	25.538803127769928	24.434868842532055	25.401326932696783	24.62500109700124
21	26.819490451841027	24.32203661294095	24.747896043785634	24.11057689143239
22	25.39322647782727	24.457556991236572	25.446366080522075	24.70285045041408
23	24.170656252056958	24.324461899642362	25.88717993724905	25.617701911051626
24	24.40880057400772	25.28828747781096	25.527852320277	24.775059627904323
25	24.716027656665883	24.68385955750212	25.620508589496875	24.97960419633512
26	24.364613194348138	25.634245760138192	26.04133569032376	23.959805355189914
27	25.0104876815817	24.85957915873874	25.330778844196054	24.799154315483506
28	24.139421448292445	25.040720828879493	26.04658673448791	24.77327098834015
29	24.08683819163749	25.159984606020767	26.051325843761642	24.701851358580104
30	24.19283712468753	25.42166501901491	26.097048662988737	24.28844919330882
31	24.831566577824272	25.211040912844645	25.04482343122379	24.912569078107296
32	24.426041900696475	25.12826296057066	25.002150148842954	25.44354498988991
33	24.060112433261676	24.7447959793985	25.710496857424076	25.48459472991575
34	24.924246114445523	25.04409194294619	25.74767603974265	24.283985902865638
35	25.34858083757704	24.852764860874863	25.850014131062533	23.948640170485564
36	24.204142265014557	25.544947678615703	25.368224495930804	24.882685560438933
37	25.135869624839668	25.341676178088928	25.284497939520957	24.237956257550447
38	24.164089791537492	25.118049183759172	25.830096058532913	24.887764966170423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	183.5
2	239.0
3	239.0
4	653.0
5	1067.0
6	1067.0
7	1321.5
8	1576.0
9	1521.0
10	1466.0
11	1466.0
12	2068.5
13	2671.0
14	3748.5
15	4826.0
16	4826.0
17	7439.5
18	10053.0
19	10053.0
20	12558.0
21	15063.0
22	16449.5
23	17836.0
24	17836.0
25	21923.5
26	26011.0
27	26011.0
28	32522.0
29	39033.0
30	47015.0
31	54997.0
32	54997.0
33	68440.5
34	81884.0
35	81884.0
36	91147.5
37	100411.0
38	114428.5
39	128446.0
40	128446.0
41	137398.5
42	146351.0
43	162953.0
44	179555.0
45	179555.0
46	185487.0
47	191419.0
48	191419.0
49	201458.0
50	211497.0
51	213030.0
52	214563.0
53	214563.0
54	204147.5
55	193732.0
56	193732.0
57	186003.0
58	178274.0
59	159910.5
60	141547.0
61	141547.0
62	131541.0
63	121535.0
64	101089.0
65	80643.0
66	80643.0
67	67059.5
68	53476.0
69	53476.0
70	42717.0
71	31958.0
72	24691.0
73	17424.0
74	17424.0
75	12989.5
76	8555.0
77	8555.0
78	8332.5
79	8110.0
80	6455.5
81	4801.0
82	4801.0
83	4747.0
84	4693.0
85	4693.0
86	3179.5
87	1666.0
88	1420.0
89	1174.0
90	1174.0
91	836.5
92	499.0
93	476.5
94	454.0
95	454.0
96	403.5
97	353.0
98	353.0
99	717.0
100	1081.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1206195342216793
2	0.05269700276472784
3	0.011539809930993692
4	0.003027554696724581
5	1.3163281290106874E-4
6	3.9489843870320617E-4
7	8.775520860071248E-5
8	4.387760430035624E-4
9	3.0714323010249366E-4
10	6.142864602049873E-4
11	0.0015795937548128247
12	0.0024571458408199492
13	0.015576549526626467
14	0.009389807320276236
15	0.02452758080389914
16	0.011101033887990129
17	0.021412270898573848
18	0.005396945328943818
19	0.0084245000256684
20	0.005572455746145243
21	0.006406130227852011
22	0.007195927105258423
23	0.009521440133177305
24	0.015444916713725398
25	0.018077572971746773
26	0.01996430995666209
27	0.008907153672972316
28	0.005923476580548093
29	0.011144911492290487
30	0.002895921883823512
31	0.00548470053754453
32	0.006801028666555217
33	0.008951031277272673
34	0.013426546915909011
35	0.01750716411584214
36	0.01588369275672896
37	0.010004093780481223
38	0.006318375019251299
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2279067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.44614531149073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29743977795454	39.490604681778265
2	14.449869693113088	15.156799313142303
3	4.842229243679057	7.618687756366252
4	2.061018858841528	4.323699782421024
5	1.061827639560005	2.7844383340060617
6	0.5820661848226568	1.8316276626068453
7	0.37597754788923143	1.380300117731962
8	0.24837966083037702	1.0421244627462989
9	0.16735616636281642	0.7899467237854461
>10	0.7736419236125109	7.144533701472652
>50	0.059521275987519176	2.1987747955927848
>100	0.0637870039680576	7.212504623140303
>500	0.01159270261809578	4.245726638131829
>1k	0.005208315668999466	4.500114252366511
>5k	8.400509143547526E-5	0.28011715471154147
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6358	0.278973808141665	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3887	0.17055224791548473	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3200	0.14040833376113998	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2915	0.12790321653553843	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2636	0.11566136493573904	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2484	0.1089919690820849	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2418	0.10609604719826139	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2391	0.10491135188215178	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2378	0.10434094302624713	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2300	0.10091848989081935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.775520860071248E-5
2	0.0	0.0	0.0	0.0	8.775520860071248E-5
3	0.0	0.0	0.0	0.0	8.775520860071248E-5
4	0.0	0.0	0.0	0.0	8.775520860071248E-5
5	0.0	4.387760430035624E-5	0.0	0.0	8.775520860071248E-5
6	0.0	4.387760430035624E-5	0.0	0.0	8.775520860071248E-5
7	0.0	4.387760430035624E-5	0.0	4.387760430035624E-5	8.775520860071248E-5
8	0.0	4.387760430035624E-5	0.0	4.387760430035624E-5	8.775520860071248E-5
9	0.0	4.387760430035624E-5	0.0	1.7551041720142497E-4	8.775520860071248E-5
10	0.0	4.387760430035624E-5	0.0	1.7551041720142497E-4	8.775520860071248E-5
11	0.0	4.387760430035624E-5	0.0	1.7551041720142497E-4	8.775520860071248E-5
12	0.0	4.387760430035624E-5	0.0	1.7551041720142497E-4	4.826536473039187E-4
13	0.0	4.387760430035624E-5	0.0	1.7551041720142497E-4	6.142864602049874E-4
14	0.0	4.387760430035624E-5	0.0	2.193880215017812E-4	6.142864602049874E-4
15	0.0	4.387760430035624E-5	0.0	2.193880215017812E-4	8.775520860071248E-4
16	0.0	4.387760430035624E-5	0.0	3.5102083440284994E-4	9.653072946078374E-4
17	0.0	4.387760430035624E-5	0.0	4.387760430035624E-4	9.653072946078374E-4
18	0.0	4.387760430035624E-5	0.0	6.581640645053436E-4	9.653072946078374E-4
19	0.0	4.387760430035624E-5	0.0	7.897968774064123E-4	9.653072946078374E-4
20	0.0	4.387760430035624E-5	0.0	8.775520860071248E-4	0.0010530625032085497
21	0.0	4.387760430035624E-5	0.0	0.001096940107508906	0.0010530625032085497
22	0.0	4.387760430035624E-5	0.0	0.0017112265677138934	0.0011408177118092623
23	0.0	4.387760430035624E-5	0.0	0.0021061250064170994	0.0011408177118092623
24	0.0	4.387760430035624E-5	0.0	0.003027554696724581	0.0011408177118092623
25	0.0	8.775520860071248E-5	0.0	0.0036857187612299243	0.0011408177118092623
26	0.0	8.775520860071248E-5	0.0	0.00443163803433598	0.0011408177118092623
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTCA	40	0.0044837217	20.000837	14
TCTAGCG	620	0.0	19.870491	28
CTAGCGG	640	0.0	18.999544	29
TAGCGGC	680	0.0	17.881924	30
ACGAACG	295	0.0	16.814262	15
AGCGGCG	755	0.0	16.74132	31
TAGCGTA	325	0.0	16.736591	7
CGAACGA	290	0.0	16.552416	16
ACCGTCG	590	0.0	16.540524	8
AAGACGG	760	0.0	16.416695	5
CAAGACG	765	0.0	16.309395	4
ATACCGT	630	0.0	16.250011	6
AATAGCG	335	0.0	16.234497	5
ATAGCGT	345	0.0	16.227932	6
GACGGAC	720	0.0	16.22041	7
TACCGTC	625	0.0	16.126196	7
AGCGTAT	350	0.0	15.998211	8
CGCAAGA	705	0.0	15.891375	2
CGTCGTA	595	0.0	15.596606	10
ATAACGA	320	0.0	15.499969	12
>>END_MODULE