FastQCFastQC Report
Thu 2 Feb 2017
SRR4063065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279067
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54160.23764110489072943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35230.15458079995015506No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT32200.1412858858471471No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32190.14124200824284674No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26660.11697769306474974No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25900.11364299513792267No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25110.11017666439819451No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24970.10956237793798955No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC23870.10473584146495035No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23160.10162053155962505No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22980.10083073468221863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6150.018.47074728
ACCGTCG5350.018.2454038
CAAGACG5900.018.1699034
CTAGCGG6450.017.61164329
TATACCG550.001363993417.4568565
ATACCGT5800.017.381616
TAGCGGC6700.016.71569630
TACCGTC5750.016.6978637
AAGACGG6550.016.6128855
CGTCGTA5800.016.55282610
CGTCTTA1551.0913936E-1116.51541515
GACGGAC6150.016.3924127
AAACGCT5400.016.29559129
AACGCTT5400.016.29559130
CGCAAGA5900.016.2715552
CCGTCGT6100.016.2633769
ATCGTTT4950.016.16091729
GCGCAAG5800.016.0017661
AGCGGCG7000.015.9993131
GTCGTAG6250.015.87305611
Produced by FastQC (version 0.11.5)