##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2279067
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.147228229797545	32.0	32.0	32.0	32.0	32.0
2	31.19183727376159	32.0	32.0	32.0	32.0	32.0
3	31.313658615565053	32.0	32.0	32.0	32.0	32.0
4	31.427612702917465	32.0	32.0	32.0	32.0	32.0
5	31.334375426435468	32.0	32.0	32.0	32.0	32.0
6	34.849764837979755	36.0	36.0	36.0	32.0	36.0
7	34.88433161464757	36.0	36.0	36.0	32.0	36.0
8	34.808635288036726	36.0	36.0	36.0	32.0	36.0
9	34.94393100334479	36.0	36.0	36.0	32.0	36.0
10	34.76607796084977	36.0	36.0	36.0	32.0	36.0
11	34.96197435178518	36.0	36.0	36.0	36.0	36.0
12	34.8474287943268	36.0	36.0	36.0	32.0	36.0
13	34.91232026087869	36.0	36.0	36.0	32.0	36.0
14	34.85488140541722	36.0	36.0	36.0	32.0	36.0
15	34.81907728030813	36.0	36.0	36.0	32.0	36.0
16	34.838548844768496	36.0	36.0	36.0	32.0	36.0
17	34.79448870963425	36.0	36.0	36.0	32.0	36.0
18	34.7987593168608	36.0	36.0	36.0	32.0	36.0
19	34.791677471526725	36.0	36.0	36.0	32.0	36.0
20	34.785501698721454	36.0	36.0	36.0	32.0	36.0
21	34.77315410209529	36.0	36.0	36.0	32.0	36.0
22	34.75764293019907	36.0	36.0	36.0	32.0	36.0
23	34.694485067793096	36.0	36.0	36.0	32.0	36.0
24	34.677900210919645	36.0	36.0	36.0	32.0	36.0
25	34.65379955920559	36.0	36.0	36.0	32.0	36.0
26	34.58530091480417	36.0	36.0	36.0	32.0	36.0
27	34.572400899139865	36.0	36.0	36.0	32.0	36.0
28	34.541833566104025	36.0	36.0	36.0	32.0	36.0
29	34.51279273492179	36.0	36.0	36.0	32.0	36.0
30	34.485098068639495	36.0	36.0	36.0	32.0	36.0
31	34.48174713599907	36.0	36.0	36.0	32.0	36.0
32	34.453681703960434	36.0	36.0	36.0	32.0	36.0
33	34.42069188839117	36.0	36.0	36.0	32.0	36.0
34	34.41731550674026	36.0	36.0	36.0	32.0	36.0
35	34.38308965905785	36.0	36.0	36.0	32.0	36.0
36	34.3534731537072	36.0	36.0	36.0	32.0	36.0
37	34.35418748110521	36.0	36.0	36.0	32.0	36.0
38	33.90612474315147	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	15.0
21	48.0
22	149.0
23	530.0
24	1505.0
25	3433.0
26	7828.0
27	15425.0
28	27023.0
29	43576.0
30	66517.0
31	97526.0
32	142391.0
33	228023.0
34	540811.0
35	1104265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91913557737076	18.527855631749325	12.296480795703427	26.256527995176494
2	15.607527115262517	20.440030942486555	37.22685642852974	26.725585513721185
3	18.34720085017246	24.198411016437866	28.4390937168587	29.015294416530974
4	11.974438617975897	16.20038586032944	36.093078602917075	35.732096918777586
5	14.179799970689746	37.0155142501358	33.17437932907603	15.630306450098416
6	34.628880377593774	35.855568713984646	16.346454661411293	13.169096247010284
7	30.38699778022226	30.516963814419736	20.417786830254794	18.67825157510321
8	27.947419481803355	33.488443931358184	18.975137987002302	19.588998599836156
9	27.08776511720971	14.30917703967368	18.25706277161131	40.3459950715053
10	15.44530196586383	27.00854610848648	31.569790732889025	25.976361192760667
11	38.031003914981454	21.13374738584376	21.831660185577668	19.003588513597123
12	24.82735199044869	23.719482470616164	28.552003260994997	22.90116227794015
13	29.45551331599288	19.499891183398095	25.15006160423753	25.89453389637149
14	23.275313977166974	19.84127715420389	25.21720511068784	31.666203757941297
15	25.13625093075368	27.39353428398551	22.228569848977674	25.241644936283137
16	25.70344667810996	26.072326183029524	23.633517970535273	24.59070916832524
17	23.881790223806494	26.223318577295007	24.992376266252812	24.902514932645683
18	24.883735318005133	25.112513146827187	26.193701194392265	23.810050340775412
19	25.329487900092452	25.173371383991782	25.498241166231622	23.998899549684147
20	25.602494354049266	24.51323282729292	25.174863222537997	24.709409596119816
21	26.86302195724038	24.43544855655782	24.52943442620793	24.172095059993875
22	25.36917551715287	24.65028421743127	25.2970845500238	24.683455715392057
23	24.174859350277703	24.302367724322156	25.69394655529941	25.82882637010073
24	24.504983837684456	25.295658267176872	25.36621345489185	24.83314444024682
25	24.69290024325776	24.81790770246031	25.487787969096086	25.001404085185847
26	24.46359558134392	25.691029299747616	25.758425388668332	24.086949730240136
27	25.082215733206382	24.938077676591057	25.132982165932084	24.84672442427048
28	24.142554826163515	25.142393795355733	25.86852426892233	24.846527109558426
29	24.16194872726427	25.11988458434965	25.889322253360696	24.828844435025385
30	24.27519682396349	25.374111423665912	25.933726388912653	24.41696536345794
31	24.78755560937875	25.207595915346058	24.938275180150473	25.066573295124716
32	24.487257285547113	25.24524289983577	24.894178187828615	25.37332162678851
33	24.056730232152017	24.849730174672356	25.555545317447887	25.537994275727744
34	24.89703023210814	25.091802917597423	25.62969846871549	24.38146838157895
35	25.40728289251698	24.90900881808214	25.58310045294851	24.100607836452372
36	24.301567264148005	25.587795356608645	25.207903058576164	24.902734320667186
37	25.196012227810765	25.3475215954599	25.101850888982202	24.354615287747137
38	24.30978304270258	24.994537235867675	25.650112809370157	25.04556691205959
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	103.0
1	100.0
2	97.0
3	97.0
4	403.0
5	709.0
6	709.0
7	969.5
8	1230.0
9	1321.0
10	1412.0
11	1412.0
12	1897.5
13	2383.0
14	3382.5
15	4382.0
16	4382.0
17	6815.5
18	9249.0
19	9249.0
20	11646.0
21	14043.0
22	15869.5
23	17696.0
24	17696.0
25	21903.5
26	26111.0
27	26111.0
28	32831.0
29	39551.0
30	47671.5
31	55792.0
32	55792.0
33	69459.5
34	83127.0
35	83127.0
36	92019.0
37	100911.0
38	114669.0
39	128427.0
40	128427.0
41	137940.0
42	147453.0
43	164335.0
44	181217.0
45	181217.0
46	185482.5
47	189748.0
48	189748.0
49	200928.5
50	212109.0
51	212754.5
52	213400.0
53	213400.0
54	204032.0
55	194664.0
56	194664.0
57	187136.5
58	179609.0
59	161029.5
60	142450.0
61	142450.0
62	132590.0
63	122730.0
64	101341.5
65	79953.0
66	79953.0
67	66858.5
68	53764.0
69	53764.0
70	43005.5
71	32247.0
72	24857.5
73	17468.0
74	17468.0
75	12898.5
76	8329.0
77	8329.0
78	8257.0
79	8185.0
80	6371.5
81	4558.0
82	4558.0
83	4258.5
84	3959.0
85	3959.0
86	2499.0
87	1039.0
88	886.5
89	734.0
90	734.0
91	435.0
92	136.0
93	92.0
94	48.0
95	48.0
96	34.5
97	21.0
98	21.0
99	22.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010004093780481223
2	0.0
3	0.0
4	1.7551041720142497E-4
5	4.387760430035624E-5
6	1.7551041720142497E-4
7	4.387760430035624E-4
8	0.0021061250064171
9	0.003290820322526718
10	7.897968774064123E-4
11	0.005660210954745956
12	3.5102083440284994E-4
13	1.3163281290106874E-4
14	0.0
15	0.0
16	1.3163281290106874E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	4.387760430035624E-5
22	8.775520860071248E-5
23	4.387760430035624E-5
24	0.0
25	1.3163281290106874E-4
26	1.3163281290106874E-4
27	8.775520860071248E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.387760430035624E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2279067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.44288307349491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38716861556884	37.01860365569994
2	15.428007375940714	15.56466344243189
3	5.243457863172448	7.934853956784157
4	2.3084677504692555	4.657830752634179
5	1.1470198285317712	2.892949354680416
6	0.6557554840197355	1.984691832312635
7	0.39840187361970647	1.4067577389082104
8	0.25547420985307706	1.0309484556729813
9	0.19188134752508612	0.8711143539473306
>10	0.8353026497919797	7.380354195113789
>50	0.06299241974627175	2.2562315827335198
>100	0.06830088648214935	7.608484336141107
>500	0.01102946603537714	3.8471718681224716
>1k	0.006652693799116314	5.3061988174196895
>5k	8.753544472521467E-5	0.2391456573977502
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5416	0.23764110489072943	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3523	0.15458079995015506	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3220	0.1412858858471471	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3219	0.14124200824284674	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2666	0.11697769306474974	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2590	0.11364299513792267	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2511	0.11017666439819451	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2497	0.10956237793798955	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2387	0.10473584146495035	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2316	0.10162053155962505	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2298	0.10083073468221863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7551041720142497E-4
2	0.0	0.0	0.0	0.0	1.7551041720142497E-4
3	0.0	0.0	0.0	0.0	1.7551041720142497E-4
4	0.0	0.0	0.0	0.0	1.7551041720142497E-4
5	0.0	0.0	0.0	0.0	2.632656258021374E-4
6	0.0	0.0	0.0	0.0	3.071432301024937E-4
7	0.0	0.0	0.0	0.0	3.071432301024937E-4
8	0.0	0.0	0.0	0.0	3.071432301024937E-4
9	0.0	0.0	0.0	1.7551041720142497E-4	3.071432301024937E-4
10	0.0	0.0	0.0	1.7551041720142497E-4	3.5102083440284994E-4
11	0.0	0.0	0.0	1.7551041720142497E-4	3.5102083440284994E-4
12	0.0	0.0	0.0	1.7551041720142497E-4	8.336744817067686E-4
13	0.0	0.0	0.0	1.7551041720142497E-4	8.336744817067686E-4
14	0.0	0.0	0.0	2.632656258021374E-4	8.336744817067686E-4
15	0.0	0.0	0.0	2.632656258021374E-4	8.775520860071248E-4
16	0.0	0.0	0.0	4.387760430035624E-4	0.0010091848989081935
17	0.0	0.0	0.0	4.826536473039187E-4	0.001096940107508906
18	0.0	0.0	0.0	7.020416688056999E-4	0.001096940107508906
19	0.0	0.0	0.0	7.459192731060562E-4	0.001096940107508906
20	0.0	0.0	0.0	8.775520860071248E-4	0.0011846953161096184
21	0.0	0.0	0.0	0.001096940107508906	0.0011846953161096184
22	0.0	4.387760430035624E-5	0.0	0.0016673489634135372	0.0012285729204099748
23	0.0	4.387760430035624E-5	0.0	0.001974492193516031	0.0012285729204099748
24	0.0	8.775520860071248E-5	0.0	0.0029836770924242246	0.0012285729204099748
25	0.0	8.775520860071248E-5	0.0	0.0036857187612299243	0.0012285729204099748
26	0.0	8.775520860071248E-5	0.0	0.004343882825735268	0.001272450524710331
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	615	0.0	18.470747	28
ACCGTCG	535	0.0	18.245403	8
CAAGACG	590	0.0	18.169903	4
CTAGCGG	645	0.0	17.611643	29
TATACCG	55	0.0013639934	17.456856	5
ATACCGT	580	0.0	17.38161	6
TAGCGGC	670	0.0	16.715696	30
TACCGTC	575	0.0	16.697863	7
AAGACGG	655	0.0	16.612885	5
CGTCGTA	580	0.0	16.552826	10
CGTCTTA	155	1.0913936E-11	16.515415	15
GACGGAC	615	0.0	16.392412	7
AAACGCT	540	0.0	16.295591	29
AACGCTT	540	0.0	16.295591	30
CGCAAGA	590	0.0	16.271555	2
CCGTCGT	610	0.0	16.263376	9
ATCGTTT	495	0.0	16.160917	29
GCGCAAG	580	0.0	16.001766	1
AGCGGCG	700	0.0	15.99931	31
GTCGTAG	625	0.0	15.873056	11
>>END_MODULE