FastQCFastQC Report
Thu 2 Feb 2017
SRR4063064_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063064_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156540
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26990.23336849568540646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18000.1556366403237242No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17620.15235097791689003No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17000.14699016030573953No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14100.12191536825358397No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13820.11949435384854826No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13560.11724626904387224No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13340.11534404343991561No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13340.11534404343991561No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12500.10808100022480847No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12090.10453594341743476No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12070.10436301381707506No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11830.10228785861275876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3050.022.0301128
CTAGCGG3200.021.49924529
TAGCGGC3200.020.99926230
ATCGTTT2750.018.0357329
AGCGGCG3750.017.91936931
TACACCG450.0088609217.7740785
AAGACGG4400.017.4509145
ACGCTTC2850.017.40289731
TAGGACG1201.6625563E-917.3304754
AACGCTT2900.017.10284830
CGCTTCG2900.017.10284832
GTACTAA753.204809E-517.0845361
TCTTGCG3850.017.0435222
TAGGGGT854.991811E-616.9383854
CGCCGGT3900.016.8184687
TCGTTTA3000.016.53275330
TAAACGC3200.016.49799328
GCGGCGC4100.016.3896732
GCGCAAG4300.016.3892351
CAAGACG4600.016.345134
Produced by FastQC (version 0.11.5)