##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063064_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1156540
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228977813132275	32.0	32.0	32.0	32.0	32.0
2	30.802108876476385	32.0	32.0	32.0	32.0	32.0
3	30.857696231864008	32.0	32.0	32.0	32.0	32.0
4	30.934796894184377	32.0	32.0	32.0	32.0	32.0
5	30.80957770591592	32.0	32.0	32.0	32.0	32.0
6	34.48615093295519	36.0	36.0	36.0	32.0	36.0
7	34.4244219828108	36.0	36.0	36.0	32.0	36.0
8	34.38631521607554	36.0	36.0	36.0	32.0	36.0
9	34.560807235374476	36.0	36.0	36.0	32.0	36.0
10	34.24567848928701	36.0	36.0	36.0	32.0	36.0
11	34.55148632991509	36.0	36.0	36.0	32.0	36.0
12	34.34442647898041	36.0	36.0	36.0	32.0	36.0
13	34.44909471354212	36.0	36.0	36.0	32.0	36.0
14	34.34202275753541	36.0	36.0	36.0	32.0	36.0
15	34.266700676154734	36.0	36.0	36.0	32.0	36.0
16	34.30122693551455	36.0	36.0	36.0	32.0	36.0
17	34.21842997215833	36.0	36.0	36.0	32.0	36.0
18	34.24047157902018	36.0	36.0	36.0	32.0	36.0
19	34.23726978746952	36.0	36.0	36.0	32.0	36.0
20	34.22620488699051	36.0	36.0	36.0	32.0	36.0
21	34.20833520673734	36.0	36.0	36.0	32.0	36.0
22	34.17315095024816	36.0	36.0	36.0	32.0	36.0
23	34.122925277119684	36.0	36.0	36.0	32.0	36.0
24	34.09808221073201	36.0	36.0	36.0	32.0	36.0
25	34.06758866965259	36.0	36.0	36.0	32.0	36.0
26	34.009795597212374	36.0	36.0	36.0	32.0	36.0
27	34.03006381102253	36.0	36.0	36.0	32.0	36.0
28	34.00809656388884	36.0	36.0	36.0	32.0	36.0
29	33.95728379476715	36.0	36.0	36.0	32.0	36.0
30	33.93242862330745	36.0	36.0	36.0	32.0	36.0
31	33.955481868331404	36.0	36.0	36.0	32.0	36.0
32	33.918265689037995	36.0	36.0	36.0	32.0	36.0
33	33.89313383021772	36.0	36.0	36.0	32.0	36.0
34	33.885587182458025	36.0	36.0	36.0	32.0	36.0
35	33.83497760561675	36.0	36.0	36.0	32.0	36.0
36	33.78006467567054	36.0	36.0	36.0	32.0	36.0
37	33.789190170681515	36.0	36.0	36.0	32.0	36.0
38	33.17662424127138	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	2.0
10	8.0
11	13.0
12	4.0
13	5.0
14	92.0
15	250.0
16	328.0
17	387.0
18	513.0
19	684.0
20	913.0
21	1392.0
22	2118.0
23	3391.0
24	5033.0
25	7835.0
26	11563.0
27	16822.0
28	23457.0
29	31780.0
30	43008.0
31	57596.0
32	78733.0
33	117423.0
34	253285.0
35	499898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78515916845079	17.29023623124292	11.146336628006132	25.778267972300164
2	16.846856121524716	19.322821008155458	37.54919271606081	26.281130154259014
3	19.225321298302838	23.417894469224372	28.36548189989294	28.99130233257985
4	12.324061184079687	14.908906970108344	36.30984600219626	36.45718584361571
5	14.432543995489992	36.67701651219717	33.895527950203146	14.99491154210969
6	34.71927881377109	35.58176594131283	16.519979006328363	13.178976238587717
7	30.478150344994553	30.337904439102843	20.784668061632107	18.399277154270496
8	28.24577574027223	33.14999818422978	19.281145214926667	19.32308086057133
9	27.239485429791326	14.086783140718248	18.697267188799678	39.97646424069075
10	15.68854125955875	26.521272217284086	31.905213171792912	25.88497335136425
11	38.12507727945201	21.021135011417904	21.850348935640376	19.003438773489712
12	24.41723626585042	23.870421049445962	28.74255846227675	22.96978422242686
13	29.26995893243352	19.137624342023905	25.53946040555627	26.052956319986304
14	23.158659333817578	19.98063020995469	25.000259416830968	31.86045103939677
15	25.092301984244013	27.333149406837048	22.30572907973041	25.26881952918853
16	25.885452787669465	25.839362027785896	23.80808257250431	24.467102612040325
17	23.93755945688835	26.198737351898295	25.1563608060192	24.707342385194153
18	25.09846681441373	24.561579481202024	26.411847665460996	23.92810603892325
19	25.207247949570462	25.35260470485488	25.403449304561747	24.03669804101291
20	25.348214223950084	24.392462593128066	25.352883597700593	24.90643958522126
21	26.667975238590998	24.380510809985843	24.555438629562023	24.396075321861137
22	25.442621930181826	24.438178200561886	25.376471839517283	24.742728029739006
23	23.939891286865358	24.20536163633502	25.948040448342237	25.906706628457382
24	24.47621205412735	25.389057614079753	25.399434789827602	24.7352955419653
25	24.744648448951743	24.680048462961377	25.358650993170734	25.21665209491615
26	24.203158998059354	25.641174211893635	26.077387150225373	24.07827963982164
27	25.204807817026243	24.90051450559903	25.09178952829781	24.802888149076914
28	24.165982839320318	24.914200925047624	25.999648935001957	24.9201673006301
29	24.103630617036274	25.242408650873603	25.947086469822683	24.70687426226744
30	24.250001945520243	25.257089691942003	26.212988639026445	24.27991972351131
31	24.746343665206496	25.106572842213392	25.02347628938007	25.123607203200038
32	24.297322280147586	25.063533912279535	25.00559893953059	25.63354486804229
33	23.93144877119527	24.765294448782438	25.72262157691346	25.580635203108827
34	24.894587742085	24.980197369103745	25.899333455550444	24.22588143326081
35	25.544446913885594	24.733711129002547	25.779495432638882	23.94234652447298
36	24.062782401902496	25.498389363312075	25.392887255431845	25.04594097935358
37	25.336508611818747	25.240091522732815	25.213371450510795	24.210028414937646
38	24.31801674045379	24.96558522412839	25.669099335915885	25.047298699501937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	80.0
2	110.0
3	110.0
4	351.5
5	593.0
6	593.0
7	694.5
8	796.0
9	814.5
10	833.0
11	833.0
12	1103.5
13	1374.0
14	1953.5
15	2533.0
16	2533.0
17	3747.0
18	4961.0
19	4961.0
20	6126.0
21	7291.0
22	8288.0
23	9285.0
24	9285.0
25	11221.0
26	13157.0
27	13157.0
28	16745.5
29	20334.0
30	24175.5
31	28017.0
32	28017.0
33	35089.0
34	42161.0
35	42161.0
36	47089.0
37	52017.0
38	58616.0
39	65215.0
40	65215.0
41	68992.5
42	72770.0
43	81813.0
44	90856.0
45	90856.0
46	93238.5
47	95621.0
48	95621.0
49	101042.5
50	106464.0
51	107160.0
52	107856.0
53	107856.0
54	102189.5
55	96523.0
56	96523.0
57	93105.0
58	89687.0
59	80187.5
60	70688.0
61	70688.0
62	67043.5
63	63399.0
64	52990.5
65	42582.0
66	42582.0
67	35496.5
68	28411.0
69	28411.0
70	22624.0
71	16837.0
72	13146.0
73	9455.0
74	9455.0
75	7103.0
76	4751.0
77	4751.0
78	4542.5
79	4334.0
80	3423.0
81	2512.0
82	2512.0
83	2489.5
84	2467.0
85	2467.0
86	1642.5
87	818.0
88	712.5
89	607.0
90	607.0
91	452.5
92	298.0
93	245.0
94	192.0
95	192.0
96	182.0
97	172.0
98	172.0
99	342.5
100	513.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12476870665951892
2	0.05421342971276393
3	0.011932142424818855
4	0.0025939440053954032
5	8.646480017984678E-5
6	8.646480017984678E-5
7	0.0
8	5.187888010790807E-4
9	1.7292960035969357E-4
10	6.917184014387743E-4
11	0.001988690404136476
12	0.0021616200044961694
13	0.013229114427516559
14	0.008646480017984678
15	0.024296608850536947
16	0.010894564822660695
17	0.020751552043163226
18	0.00510142321061096
19	0.006484860013488509
20	0.005879606412229582
21	0.005447282411330348
22	0.006830719214207896
23	0.008819409618344373
24	0.013488508828056099
25	0.016687706434710427
26	0.01936811524028568
27	0.00778183201618621
28	0.00510142321061096
29	0.010808100022480848
30	0.003199197606654331
31	0.004669099209711727
32	0.006139000812769122
33	0.007868296816366057
34	0.011067494423020388
35	0.01582305843291196
36	0.014266692029674719
37	0.00916526881906376
38	0.0051878880107908065
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1156540.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.29837970778641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.49577999894126	46.69576146961561
2	11.765257603461684	13.482603950461373
3	3.2481851812590397	5.583472436309564
4	1.2293844589802438	2.817669501500063
5	0.6341411252730992	1.8167629492110489
6	0.3502636220926923	1.2041722809895012
7	0.22568154118330228	0.9051830647831502
8	0.1513560658498433	0.6937965865712834
9	0.11316057030050378	0.583552559252513
>10	0.6125673689727638	6.650896612533785
>50	0.0787832875050091	3.185551707255381
>100	0.08299718724710915	10.258541740402128
>500	0.008952162769175277	3.477328479474556
>1k	0.0034898261642547683	2.644706661640023
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2699	0.23336849568540646	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1800	0.1556366403237242	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1762	0.15235097791689003	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1700	0.14699016030573953	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1410	0.12191536825358397	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1382	0.11949435384854826	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1356	0.11724626904387224	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1334	0.11534404343991561	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1334	0.11534404343991561	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1250	0.10808100022480847	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1209	0.10453594341743476	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1207	0.10436301381707506	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1183	0.10228785861275876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.646480017984678E-5	0.0
8	0.0	0.0	0.0	8.646480017984678E-5	0.0
9	0.0	0.0	0.0	8.646480017984678E-5	0.0
10	0.0	0.0	0.0	8.646480017984678E-5	0.0
11	0.0	0.0	0.0	8.646480017984678E-5	0.0
12	0.0	0.0	0.0	8.646480017984678E-5	8.646480017984678E-5
13	0.0	0.0	0.0	8.646480017984678E-5	8.646480017984678E-5
14	0.0	0.0	0.0	8.646480017984678E-5	8.646480017984678E-5
15	0.0	0.0	0.0	8.646480017984678E-5	1.7292960035969357E-4
16	0.0	0.0	0.0	8.646480017984678E-5	1.7292960035969357E-4
17	0.0	0.0	0.0	1.7292960035969357E-4	1.7292960035969357E-4
18	0.0	0.0	0.0	2.5939440053954037E-4	1.7292960035969357E-4
19	0.0	0.0	0.0	3.4585920071938714E-4	1.7292960035969357E-4
20	0.0	0.0	0.0	6.917184014387743E-4	1.7292960035969357E-4
21	0.0	0.0	0.0	6.917184014387743E-4	1.7292960035969357E-4
22	0.0	0.0	0.0	0.001210507202517855	1.7292960035969357E-4
23	0.0	0.0	0.0	0.0014699016030573953	1.7292960035969357E-4
24	0.0	0.0	0.0	0.001988690404136476	1.7292960035969357E-4
25	0.0	0.0	0.0	0.00268040880557525	1.7292960035969357E-4
26	0.0	0.0	0.0	0.0034585920071938713	2.5939440053954037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	305	0.0	22.03011	28
CTAGCGG	320	0.0	21.499245	29
TAGCGGC	320	0.0	20.999262	30
ATCGTTT	275	0.0	18.03573	29
AGCGGCG	375	0.0	17.919369	31
TACACCG	45	0.00886092	17.774078	5
AAGACGG	440	0.0	17.450914	5
ACGCTTC	285	0.0	17.402897	31
TAGGACG	120	1.6625563E-9	17.330475	4
AACGCTT	290	0.0	17.102848	30
CGCTTCG	290	0.0	17.102848	32
GTACTAA	75	3.204809E-5	17.084536	1
TCTTGCG	385	0.0	17.043522	2
TAGGGGT	85	4.991811E-6	16.938385	4
CGCCGGT	390	0.0	16.818468	7
TCGTTTA	300	0.0	16.532753	30
TAAACGC	320	0.0	16.497993	28
GCGGCGC	410	0.0	16.38967	32
GCGCAAG	430	0.0	16.389235	1
CAAGACG	460	0.0	16.34513	4
>>END_MODULE