FastQCFastQC Report
Thu 2 Feb 2017
SRR4063064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156540
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25400.21962059245681084No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19350.16730938834800352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17840.15425320352084668No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17290.14949763951095507No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15050.13012952427066943No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15010.12978366506995004No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14340.12399052345790028No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14250.12321234025628168No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13890.12009960744980717No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12950.11197191623290158No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12870.11128019783146281No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12610.1090321130267868No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11870.10263371781347812No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11660.10081795700970135No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11650.10073149220952152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC308.4085925E-426.6711737
CGCTTAA401.5940715E-423.99886528
TCTAGCG2950.021.69388828
GATAACG1800.020.4461311
CTAGCGG3100.020.1280829
TCGCGTA1850.019.0294199
ACCGTCG3050.018.8884358
CGATAAC1950.018.8733510
ATAACGA1950.018.87090112
ACGAACG1900.018.5254415
CGTCGTA3250.018.21688710
CGCGTAA1950.018.0527710
CGAACGA1950.018.05042816
TAACGAA1950.018.05042813
TACCGTC3300.017.9424257
GTTTTCG2900.017.65433728
CCGATAA2100.017.526019
CAAGACG4300.017.4890564
TAGCGGC3500.017.37060730
CCGTCGT3350.017.196199
Produced by FastQC (version 0.11.5)