##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1156540
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.133579469797844	32.0	32.0	32.0	32.0	32.0
2	31.19838916077265	32.0	32.0	32.0	32.0	32.0
3	31.333195566085045	32.0	32.0	32.0	32.0	32.0
4	31.442343541944073	32.0	32.0	32.0	32.0	32.0
5	31.352268836356718	32.0	32.0	32.0	32.0	32.0
6	34.878890483684096	36.0	36.0	36.0	36.0	36.0
7	34.9085600152178	36.0	36.0	36.0	36.0	36.0
8	34.83655645286804	36.0	36.0	36.0	32.0	36.0
9	34.96564321164854	36.0	36.0	36.0	32.0	36.0
10	34.79176941567088	36.0	36.0	36.0	32.0	36.0
11	34.98987929513895	36.0	36.0	36.0	36.0	36.0
12	34.88414927283103	36.0	36.0	36.0	32.0	36.0
13	34.941861933007075	36.0	36.0	36.0	36.0	36.0
14	34.8805298562955	36.0	36.0	36.0	32.0	36.0
15	34.84839175471666	36.0	36.0	36.0	32.0	36.0
16	34.86090926383869	36.0	36.0	36.0	32.0	36.0
17	34.82143981185259	36.0	36.0	36.0	32.0	36.0
18	34.82695799540007	36.0	36.0	36.0	32.0	36.0
19	34.81946236187248	36.0	36.0	36.0	32.0	36.0
20	34.80934252165943	36.0	36.0	36.0	32.0	36.0
21	34.80361595794352	36.0	36.0	36.0	32.0	36.0
22	34.77940149065316	36.0	36.0	36.0	32.0	36.0
23	34.71993186573746	36.0	36.0	36.0	32.0	36.0
24	34.696550919120824	36.0	36.0	36.0	32.0	36.0
25	34.6696638248569	36.0	36.0	36.0	32.0	36.0
26	34.606209901948915	36.0	36.0	36.0	32.0	36.0
27	34.59169332664672	36.0	36.0	36.0	32.0	36.0
28	34.57278001625538	36.0	36.0	36.0	32.0	36.0
29	34.523762256385425	36.0	36.0	36.0	32.0	36.0
30	34.50869317101008	36.0	36.0	36.0	32.0	36.0
31	34.49928493610251	36.0	36.0	36.0	32.0	36.0
32	34.46425372230965	36.0	36.0	36.0	32.0	36.0
33	34.43896276825704	36.0	36.0	36.0	32.0	36.0
34	34.41946927905649	36.0	36.0	36.0	32.0	36.0
35	34.39530236740623	36.0	36.0	36.0	32.0	36.0
36	34.36326456499559	36.0	36.0	36.0	32.0	36.0
37	34.35599114600446	36.0	36.0	36.0	32.0	36.0
38	33.89510090442181	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	23.0
22	89.0
23	266.0
24	735.0
25	1913.0
26	3977.0
27	7673.0
28	13656.0
29	21485.0
30	33185.0
31	48476.0
32	70277.0
33	114038.0
34	271977.0
35	568766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11096237956636	18.494335230525248	11.868258440082807	26.526443949825584
2	15.815449530496133	20.466650526570636	36.70214605634046	27.01575388659277
3	18.17853251941135	24.144690196620957	28.71314437892334	28.963632905044356
4	11.854797429565531	15.801842744194733	36.30444707298346	36.03891275325628
5	14.004098431528526	37.202258460580694	33.44743804797067	15.346205059920107
6	34.94613242948795	35.97705224203227	16.09499022947758	12.981825099002196
7	30.334498304422336	30.515728839000534	20.695472176443836	18.454300680133294
8	28.146589814492962	33.49701601202242	19.079537849349677	19.276856324134936
9	26.971984435797662	14.019628188499784	18.433030696065718	40.57535667963684
10	15.5007055600902	26.802550392208403	31.734640233526555	25.962103814174842
11	38.24519230769231	20.924875484228004	21.71347537354732	19.116456834532375
12	24.27116469252605	24.014277104839707	28.64958060139883	23.064977601235412
13	29.322166673295648	19.07779943244407	25.537941684579323	26.06209220968096
14	23.03577913431442	19.901170733394437	25.003804451207913	32.05924568108323
15	25.06294637453093	27.4824044131634	22.153838172479983	25.30081103982569
16	25.90416241548066	25.957424732391445	23.63575838276238	24.50265446936552
17	23.953343593822954	26.35516281321874	24.94924516229443	24.742248430663878
18	25.190309025195845	24.654918982482233	26.184827156864444	23.969944835457486
19	25.303750843031803	25.404914659242223	25.279713628581806	24.01162086914417
20	25.41624154806578	24.432790910820206	25.138689539488475	25.01227800162554
21	26.571220808326927	24.5795854347937	24.440982000575858	24.408211756303515
22	25.443413494918893	24.666699523319142	25.21341692757443	24.67647005418754
23	23.858273571642265	24.196092130133206	25.9361127779632	26.00952152026133
24	24.449478617254915	25.42791429609006	25.31231085824961	24.810296228405416
25	24.707403116191397	24.790322859563872	25.37127985197226	25.13099417227247
26	24.348574195445032	25.699932557455863	25.753108409566465	24.19838483753264
27	25.360666609599846	24.890902944042526	24.923067877520776	24.825362568836848
28	24.263146972867343	24.923046327839938	25.851591816971307	24.96221488232141
29	24.078285230082834	25.15970048593218	25.966244142009785	24.7957701419752
30	24.206512528749546	25.198955505213828	26.15378629359987	24.44074567243675
31	24.76758261711657	25.150448752312933	24.987116744773203	25.094851885797294
32	24.5246165286112	25.0579314161205	24.90999014301278	25.50746191225552
33	24.00556833313158	24.7082677641932	25.624708181299393	25.661455721375827
34	24.94881283829353	24.859927023708646	25.82937036332509	24.361889774672733
35	25.609663306068096	24.703512200183305	25.607069362062706	24.07975513168589
36	24.112352361353693	25.494232797828005	25.444515537724595	24.94889930309371
37	25.50383039064797	25.11733273384405	25.125201030660417	24.25363584484756
38	24.365521296280285	24.74596641707161	25.719473602296507	25.1690386843516
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	50.5
2	66.0
3	66.0
4	256.0
5	446.0
6	446.0
7	570.0
8	694.0
9	745.5
10	797.0
11	797.0
12	1067.5
13	1338.0
14	1926.0
15	2514.0
16	2514.0
17	3720.5
18	4927.0
19	4927.0
20	5956.5
21	6986.0
22	8243.5
23	9501.0
24	9501.0
25	11383.5
26	13266.0
27	13266.0
28	16998.0
29	20730.0
30	24311.0
31	27892.0
32	27892.0
33	35239.0
34	42586.0
35	42586.0
36	47027.0
37	51468.0
38	58558.5
39	65649.0
40	65649.0
41	69099.5
42	72550.0
43	81495.5
44	90441.0
45	90441.0
46	93582.5
47	96724.0
48	96724.0
49	101915.0
50	107106.0
51	106881.5
52	106657.0
53	106657.0
54	101685.0
55	96713.0
56	96713.0
57	93464.5
58	90216.0
59	80316.0
60	70416.0
61	70416.0
62	67071.0
63	63726.0
64	53388.5
65	43051.0
66	43051.0
67	35904.5
68	28758.0
69	28758.0
70	23024.5
71	17291.0
72	13419.0
73	9547.0
74	9547.0
75	7040.5
76	4534.0
77	4534.0
78	4463.0
79	4392.0
80	3350.0
81	2308.0
82	2308.0
83	2234.5
84	2161.0
85	2161.0
86	1361.0
87	561.0
88	467.0
89	373.0
90	373.0
91	220.0
92	67.0
93	44.5
94	22.0
95	22.0
96	18.0
97	14.0
98	14.0
99	15.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008127691216905597
2	0.0
3	0.0
4	3.4585920071938714E-4
5	0.0
6	0.0
7	1.7292960035969357E-4
8	0.002939803206114791
9	0.0034585920071938717
10	0.0010375776021581615
11	0.0051878880107908065
12	2.5939440053954037E-4
13	1.7292960035969357E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.5939440053954037E-4
22	8.646480017984678E-5
23	1.7292960035969357E-4
24	0.0
25	0.0
26	0.0
27	8.646480017984678E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1156540.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.3779513512242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.05281047561763	44.331606440475234
2	12.696458103969757	14.062076784289868
3	3.483209356053195	5.786789947969283
4	1.344897048133779	2.9791057321582977
5	0.6619727719276003	1.8329347979820838
6	0.39322182565125435	1.3065491478692837
7	0.22617844718570093	0.8767709331461624
8	0.16712648622892043	0.7404097939108956
9	0.12989788852835893	0.6474131056395183
>10	0.6609181042770467	6.9249965489941845
>50	0.07996793152274188	3.159998978958267
>100	0.0885784832814893	10.377654866582334
>500	0.010679673173756406	3.993726837525186
>1k	0.004083404448789214	2.9799660844993454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2540	0.21962059245681084	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1935	0.16730938834800352	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1784	0.15425320352084668	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1729	0.14949763951095507	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1505	0.13012952427066943	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1501	0.12978366506995004	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1434	0.12399052345790028	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1425	0.12321234025628168	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1389	0.12009960744980717	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1295	0.11197191623290158	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1287	0.11128019783146281	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1261	0.1090321130267868	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1187	0.10263371781347812	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1166	0.10081795700970135	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1165	0.10073149220952152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.646480017984678E-5	0.0
11	0.0	0.0	0.0	8.646480017984678E-5	0.0
12	0.0	0.0	0.0	8.646480017984678E-5	8.646480017984678E-5
13	0.0	0.0	0.0	8.646480017984678E-5	1.7292960035969357E-4
14	0.0	0.0	0.0	8.646480017984678E-5	1.7292960035969357E-4
15	0.0	0.0	0.0	8.646480017984678E-5	2.5939440053954037E-4
16	0.0	0.0	0.0	8.646480017984678E-5	2.5939440053954037E-4
17	0.0	0.0	0.0	1.7292960035969357E-4	2.5939440053954037E-4
18	0.0	0.0	0.0	2.5939440053954037E-4	2.5939440053954037E-4
19	0.0	0.0	0.0	3.4585920071938714E-4	3.4585920071938714E-4
20	0.0	0.0	0.0	6.917184014387743E-4	3.4585920071938714E-4
21	0.0	0.0	0.0	7.781832016186211E-4	3.4585920071938714E-4
22	0.0	0.0	0.0	0.0012969720026977018	3.4585920071938714E-4
23	0.0	0.0	0.0	0.001642831203417089	3.4585920071938714E-4
24	0.0	0.0	0.0	0.0021616200044961694	3.4585920071938714E-4
25	0.0	0.0	0.0	0.0025074792052155567	3.4585920071938714E-4
26	0.0	0.0	0.0	0.003199197606654331	3.4585920071938714E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTC	30	8.4085925E-4	26.671173	7
CGCTTAA	40	1.5940715E-4	23.998865	28
TCTAGCG	295	0.0	21.693888	28
GATAACG	180	0.0	20.44613	11
CTAGCGG	310	0.0	20.12808	29
TCGCGTA	185	0.0	19.029419	9
ACCGTCG	305	0.0	18.888435	8
CGATAAC	195	0.0	18.87335	10
ATAACGA	195	0.0	18.870901	12
ACGAACG	190	0.0	18.52544	15
CGTCGTA	325	0.0	18.216887	10
CGCGTAA	195	0.0	18.05277	10
CGAACGA	195	0.0	18.050428	16
TAACGAA	195	0.0	18.050428	13
TACCGTC	330	0.0	17.942425	7
GTTTTCG	290	0.0	17.654337	28
CCGATAA	210	0.0	17.52601	9
CAAGACG	430	0.0	17.489056	4
TAGCGGC	350	0.0	17.370607	30
CCGTCGT	335	0.0	17.19619	9
>>END_MODULE