FastQCFastQC Report
Thu 2 Feb 2017
SRR4063063_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063063_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775801
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28790.3711003208296973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20740.26733659791621817No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14730.18986827807646547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12800.16499076438416552No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12160.15674122616495725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.10879078526580914No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8120.10466601615620501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTATT350.002066277422.85649911
GTCTAAA400.00445788820.0187951
ACCGTCG1003.261448E-919.1982218
CTTATAC550.001364951317.4529293
GTATAGG805.572128E-516.0150361
GATATAT600.002425690416.0150341
GTATAAC1001.3002191E-616.0150341
GCGTCCA600.002441404915.9995499
GTCGTAG1304.793037E-915.99954811
TACCGTC1203.1088348E-815.9985177
TAATACT1203.1111995E-815.9974864
ATACCGT1407.4396667E-1015.9964556
GGCATCG1551.891749E-1015.48343526
CGTTTAT1357.898052E-915.40697331
GTTATTC951.4324538E-515.156493
GTACATA859.350708E-515.0729731
TCTTTCG1704.7293724E-1115.05839910
TCATAGG756.2455E-414.931953
CTTACAC756.2455E-414.931953
GTATTAC650.004134064514.7831081
Produced by FastQC (version 0.11.5)