##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063063_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775801
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22025880348182	32.0	32.0	32.0	32.0	32.0
2	30.851665568876555	32.0	32.0	32.0	32.0	32.0
3	30.911791812591115	32.0	32.0	32.0	32.0	32.0
4	30.97756641200514	32.0	32.0	32.0	32.0	32.0
5	30.893754970669026	32.0	32.0	32.0	32.0	32.0
6	34.5572859534855	36.0	36.0	36.0	32.0	36.0
7	34.479229854047624	36.0	36.0	36.0	32.0	36.0
8	34.45973387505301	36.0	36.0	36.0	32.0	36.0
9	34.583199815416584	36.0	36.0	36.0	32.0	36.0
10	34.33247701407964	36.0	36.0	36.0	32.0	36.0
11	34.59455066441007	36.0	36.0	36.0	32.0	36.0
12	34.4093498203792	36.0	36.0	36.0	32.0	36.0
13	34.4979859525832	36.0	36.0	36.0	32.0	36.0
14	34.410362966791745	36.0	36.0	36.0	32.0	36.0
15	34.35482681770196	36.0	36.0	36.0	32.0	36.0
16	34.35837411913622	36.0	36.0	36.0	32.0	36.0
17	34.29628732110425	36.0	36.0	36.0	32.0	36.0
18	34.321470325508734	36.0	36.0	36.0	32.0	36.0
19	34.30872221097936	36.0	36.0	36.0	32.0	36.0
20	34.28429197693738	36.0	36.0	36.0	32.0	36.0
21	34.25656708356911	36.0	36.0	36.0	32.0	36.0
22	34.24727346316904	36.0	36.0	36.0	32.0	36.0
23	34.17992758452232	36.0	36.0	36.0	32.0	36.0
24	34.16493791577995	36.0	36.0	36.0	32.0	36.0
25	34.142245240725394	36.0	36.0	36.0	32.0	36.0
26	34.10242961790459	36.0	36.0	36.0	32.0	36.0
27	34.09333321302757	36.0	36.0	36.0	32.0	36.0
28	34.07574622873649	36.0	36.0	36.0	32.0	36.0
29	34.049355440377106	36.0	36.0	36.0	32.0	36.0
30	34.02862331963996	36.0	36.0	36.0	32.0	36.0
31	34.03480145037194	36.0	36.0	36.0	32.0	36.0
32	33.976658962801025	36.0	36.0	36.0	32.0	36.0
33	33.94428983721341	36.0	36.0	36.0	32.0	36.0
34	33.927262274732826	36.0	36.0	36.0	32.0	36.0
35	33.8809282277285	36.0	36.0	36.0	32.0	36.0
36	33.84640391028112	36.0	36.0	36.0	32.0	36.0
37	33.83340830960517	36.0	36.0	36.0	32.0	36.0
38	33.2235998664606	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	0.0
9	5.0
10	2.0
11	14.0
12	4.0
13	3.0
14	179.0
15	327.0
16	407.0
17	398.0
18	492.0
19	661.0
20	844.0
21	1076.0
22	1643.0
23	2407.0
24	3428.0
25	5130.0
26	7333.0
27	10741.0
28	14817.0
29	19766.0
30	26456.0
31	35994.0
32	48798.0
33	73359.0
34	163441.0
35	358073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.538677954727305	17.333711896342567	11.581446971065741	25.54616317786439
2	16.194541890218737	20.26452228642179	38.05857924061081	25.48235658274866
3	18.299375414939764	25.142868192570727	29.08001005523936	27.477746337250146
4	12.395217685540267	15.932712448841482	36.567819277496696	35.10425058812155
5	14.10429763379432	37.16877696413633	33.864312792359875	14.862612609709474
6	32.915571023459655	36.16731116267079	17.5041247744264	13.412993039443155
7	29.053622067543184	31.07837071410157	21.822248002062388	18.045759216292858
8	27.862830096017387	33.100798275837626	20.0183810971169	19.017990531028094
9	28.060783863840843	13.98456814789417	18.743796709968315	39.21085127829667
10	15.608722384850676	27.23470186144056	32.233561099101955	24.923014654606813
11	36.681825564398245	21.553864358801828	22.428336826582726	19.3359732502172
12	24.08762323144825	24.501531369879963	29.31129604421895	22.099549354452833
13	29.752022432075286	19.948947690720985	25.20308118735295	25.09594868985078
14	23.690021773348338	19.84713536731729	25.914022990283854	30.54881986905052
15	25.06182358295706	26.889197183062276	23.443837884253877	24.60514134972679
16	25.142515334417936	25.888416487689923	24.35768190525792	24.61138627263422
17	23.60021660091282	25.983729145715685	25.932415358036152	24.483638895335343
18	24.566356258185607	24.983499881405397	26.90357227567573	23.546571584733265
19	25.169677789451296	25.560919643950577	26.17130850097005	23.098094065628082
20	25.496939727849764	24.15629817930565	26.093654124793105	24.25310796805148
21	26.555978085723492	24.612826297131807	24.964099258781825	23.867096358362875
22	25.366937116014775	24.732966718487752	25.45614157211252	24.443954593384948
23	24.017810642039098	24.572008312320488	25.843349885782942	25.566831159857472
24	24.19638460090604	25.612170966968424	25.847447794328087	24.34399663779745
25	24.661253600187713	24.65222895775694	25.831105106142687	24.855412335912664
26	24.205977265196825	25.411821736658418	26.23926852569301	24.142932472451747
27	25.03351690283192	25.35592372584205	25.311191551677908	24.299367819648126
28	24.103930523919466	24.836770656488117	26.257977773738673	24.80132104585375
29	24.06771663897136	25.57950658950655	26.211191654537885	24.141585116984206
30	24.318387771649107	25.388420036325154	26.371055375877678	23.922136816148065
31	24.351816640777574	25.40061489278049	25.34569994005762	24.901868526384312
32	24.130836639827987	25.574865999953595	25.33677260340369	24.95752475681473
33	23.81350775783389	24.997937431999713	25.861508871620607	25.32704593854579
34	24.378915017294077	25.309559506973713	26.11580007193512	24.19572540379709
35	25.26809219432075	25.06052862959057	25.886528480041665	23.78485069604701
36	24.181777161826794	25.725078157733588	25.558384632739227	24.53476004770039
37	24.994488983768605	25.678241734442498	25.54623517020983	23.781034111579064
38	24.074704286937063	25.69674328909239	25.70976291392094	24.518789510049604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	430.0
1	343.5
2	257.0
3	257.0
4	622.5
5	988.0
6	988.0
7	1155.5
8	1323.0
9	1179.0
10	1035.0
11	1035.0
12	1407.5
13	1780.0
14	2184.0
15	2588.0
16	2588.0
17	3789.5
18	4991.0
19	4991.0
20	5864.5
21	6738.0
22	7179.5
23	7621.0
24	7621.0
25	8404.0
26	9187.0
27	9187.0
28	11537.0
29	13887.0
30	16480.0
31	19073.0
32	19073.0
33	23366.0
34	27659.0
35	27659.0
36	30574.5
37	33490.0
38	37849.0
39	42208.0
40	42208.0
41	45972.5
42	49737.0
43	55853.0
44	61969.0
45	61969.0
46	65640.0
47	69311.0
48	69311.0
49	71284.0
50	73257.0
51	72400.0
52	71543.0
53	71543.0
54	67822.0
55	64101.0
56	64101.0
57	61709.0
58	59317.0
59	52779.0
60	46241.0
61	46241.0
62	43280.5
63	40320.0
64	32869.5
65	25419.0
66	25419.0
67	21059.0
68	16699.0
69	16699.0
70	13262.0
71	9825.0
72	7638.5
73	5452.0
74	5452.0
75	4069.5
76	2687.0
77	2687.0
78	2554.0
79	2421.0
80	1880.5
81	1340.0
82	1340.0
83	1362.5
84	1385.0
85	1385.0
86	914.5
87	444.0
88	390.5
89	337.0
90	337.0
91	254.5
92	172.0
93	155.5
94	139.0
95	139.0
96	119.0
97	99.0
98	99.0
99	215.0
100	331.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1212939916292967
2	0.05684447429173203
3	0.011085316982061121
4	0.003351374901553362
5	2.5779806935025866E-4
6	1.2889903467512933E-4
7	1.2889903467512933E-4
8	5.155961387005173E-4
9	3.86697104025388E-4
10	5.155961387005173E-4
11	0.002449081658827457
12	0.0032224758668782326
13	0.016241278369066296
14	0.010569720843360605
15	0.02668210017775177
16	0.012245408294137285
17	0.023330725276198408
18	0.006316052699081337
19	0.008507336288558535
20	0.0068316488377818535
21	0.006573850768431594
22	0.007089446907132112
23	0.010956417947385992
24	0.01533898512634039
25	0.01894815809724401
26	0.02088164361737095
27	0.00940962953128444
28	0.006702749803106724
29	0.012632105398162674
30	0.0038669710402538795
31	0.007218345941807242
32	0.007089446907132112
33	0.008894033392583923
34	0.013147701536863189
35	0.01740136968114246
36	0.016241278369066296
37	0.010569720843360605
38	0.006316052699081337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	775801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.55772031433677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.16903665300951	50.58138576784445
2	11.4108553571337	14.048524852435756
3	2.985430179244557	5.513288279757502
4	1.226744227143208	3.0206231212683528
5	0.5781225963960902	1.779395454817436
6	0.35140043103792346	1.2978845671301926
7	0.23096914401441482	0.995255377793678
8	0.15727069615109399	0.7744980421848067
9	0.1171530745840233	0.6490508579287148
>10	0.6273998503863484	7.314187042843794
>50	0.07438542031417518	3.2136946297458677
>100	0.06471849599098607	7.548370093198106
>500	0.00546324965945597	2.1097977031577773
>1k	0.0010506249345107633	1.1540442098935497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2879	0.3711003208296973	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2074	0.26733659791621817	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1473	0.18986827807646547	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1280	0.16499076438416552	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1216	0.15674122616495725	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.10879078526580914	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	812	0.10466601615620501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.577980693502586E-4
2	0.0	0.0	0.0	0.0	2.577980693502586E-4
3	0.0	0.0	0.0	0.0	2.577980693502586E-4
4	0.0	0.0	0.0	0.0	2.577980693502586E-4
5	0.0	0.0	0.0	0.0	2.577980693502586E-4
6	0.0	0.0	0.0	0.0	6.444951733756466E-4
7	0.0	0.0	0.0	0.0	7.733942080507759E-4
8	0.0	0.0	0.0	1.288990346751293E-4	7.733942080507759E-4
9	0.0	0.0	0.0	1.288990346751293E-4	7.733942080507759E-4
10	0.0	0.0	0.0	2.577980693502586E-4	7.733942080507759E-4
11	0.0	0.0	0.0	2.577980693502586E-4	7.733942080507759E-4
12	0.0	0.0	0.0	2.577980693502586E-4	0.0010311922774010344
13	0.0	0.0	0.0	2.577980693502586E-4	0.0014178893814264224
14	0.0	0.0	0.0	2.577980693502586E-4	0.0015467884161015518
15	0.0	0.0	0.0	2.577980693502586E-4	0.0015467884161015518
16	0.0	0.0	0.0	2.577980693502586E-4	0.0015467884161015518
17	0.0	0.0	0.0	2.577980693502586E-4	0.0015467884161015518
18	0.0	0.0	0.0	3.8669710402538794E-4	0.0015467884161015518
19	0.0	0.0	0.0	3.8669710402538794E-4	0.0015467884161015518
20	0.0	0.0	0.0	3.8669710402538794E-4	0.002062384554802069
21	0.0	0.0	0.0	5.155961387005172E-4	0.002062384554802069
22	0.0	0.0	0.0	7.733942080507759E-4	0.002062384554802069
23	0.0	0.0	0.0	0.0015467884161015518	0.0021912835894771984
24	0.0	0.0	0.0	0.0029646777975279744	0.0021912835894771984
25	0.0	0.0	0.0	0.0037380720055787504	0.0021912835894771984
26	0.0	0.0	0.0	0.004640365248304655	0.0021912835894771984
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTATT	35	0.0020662774	22.856499	11
GTCTAAA	40	0.004457888	20.018795	1
ACCGTCG	100	3.261448E-9	19.198221	8
CTTATAC	55	0.0013649513	17.452929	3
GTATAGG	80	5.572128E-5	16.015036	1
GATATAT	60	0.0024256904	16.015034	1
GTATAAC	100	1.3002191E-6	16.015034	1
GCGTCCA	60	0.0024414049	15.999549	9
GTCGTAG	130	4.793037E-9	15.999548	11
TACCGTC	120	3.1088348E-8	15.998517	7
TAATACT	120	3.1111995E-8	15.997486	4
ATACCGT	140	7.4396667E-10	15.996455	6
GGCATCG	155	1.891749E-10	15.483435	26
CGTTTAT	135	7.898052E-9	15.406973	31
GTTATTC	95	1.4324538E-5	15.15649	3
GTACATA	85	9.350708E-5	15.072973	1
TCTTTCG	170	4.7293724E-11	15.058399	10
TCATAGG	75	6.2455E-4	14.93195	3
CTTACAC	75	6.2455E-4	14.93195	3
GTATTAC	65	0.0041340645	14.783108	1
>>END_MODULE