FastQCFastQC Report
Thu 2 Feb 2017
SRR4063063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775801
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33830.4360654343059625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27530.354859042460631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15840.20417607092540485No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15200.19592653270619656No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13630.17568938426220126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.13560178447823604No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8080.10415042001750449No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7780.10028344897725061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1300.019.69167328
CTAGCGG1401.8189894E-1218.28512629
CTAAGAC854.9812497E-616.9406323
AAGCTCG2100.016.76136424
CGCTTCG1551.0913936E-1116.51559832
CGAGCCG1750.016.45661215
TTTCGTT2350.016.339928
CTAGACA1203.10647E-815.9994844
CTTTCGT2400.015.99948427
CGACTTT2550.015.68576924
TAGAGTG1950.015.591255
TAAACGC1652.910383E-1115.51465128
AACGCTT1357.898052E-915.40691130
AGGCCCG2100.015.23956810
CGCGCAA1052.1833384E-615.23760421
TAGCGGC1704.7293724E-1115.05833930
TCGTTCT2550.015.05833830
TCCGACT2550.015.05833822
TTCGTTC2550.015.05833829
CCGACTT2550.015.05833823
Produced by FastQC (version 0.11.5)