##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775801
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14096914028211	32.0	32.0	32.0	32.0	32.0
2	31.303443795509416	32.0	32.0	32.0	32.0	32.0
3	31.388225846576635	32.0	32.0	32.0	32.0	32.0
4	31.48964618503972	32.0	32.0	32.0	32.0	32.0
5	31.42083859133979	32.0	32.0	32.0	32.0	32.0
6	34.940997755867805	36.0	36.0	36.0	36.0	36.0
7	34.9733385236678	36.0	36.0	36.0	36.0	36.0
8	34.90421899430395	36.0	36.0	36.0	36.0	36.0
9	35.01658543879165	36.0	36.0	36.0	36.0	36.0
10	34.86663203579268	36.0	36.0	36.0	32.0	36.0
11	35.04342350680136	36.0	36.0	36.0	36.0	36.0
12	34.94163065012806	36.0	36.0	36.0	36.0	36.0
13	34.99157387010329	36.0	36.0	36.0	36.0	36.0
14	34.94169638863575	36.0	36.0	36.0	32.0	36.0
15	34.91239763805409	36.0	36.0	36.0	32.0	36.0
16	34.92597457337642	36.0	36.0	36.0	36.0	36.0
17	34.899991105966606	36.0	36.0	36.0	32.0	36.0
18	34.888332188280245	36.0	36.0	36.0	32.0	36.0
19	34.8800955399645	36.0	36.0	36.0	32.0	36.0
20	34.86236547774494	36.0	36.0	36.0	32.0	36.0
21	34.854560641195356	36.0	36.0	36.0	32.0	36.0
22	34.8348455338418	36.0	36.0	36.0	32.0	36.0
23	34.778456073142465	36.0	36.0	36.0	32.0	36.0
24	34.75853859430447	36.0	36.0	36.0	32.0	36.0
25	34.74353861363932	36.0	36.0	36.0	32.0	36.0
26	34.671012282789015	36.0	36.0	36.0	32.0	36.0
27	34.65218657877471	36.0	36.0	36.0	32.0	36.0
28	34.620156457648285	36.0	36.0	36.0	32.0	36.0
29	34.59151380315313	36.0	36.0	36.0	32.0	36.0
30	34.569470779233335	36.0	36.0	36.0	32.0	36.0
31	34.55198433618931	36.0	36.0	36.0	32.0	36.0
32	34.51952240329672	36.0	36.0	36.0	32.0	36.0
33	34.47309425999709	36.0	36.0	36.0	32.0	36.0
34	34.45290093722488	36.0	36.0	36.0	32.0	36.0
35	34.42084761427222	36.0	36.0	36.0	32.0	36.0
36	34.380004666145055	36.0	36.0	36.0	32.0	36.0
37	34.37386520512348	36.0	36.0	36.0	32.0	36.0
38	33.94282038821811	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	2.0
21	22.0
22	63.0
23	164.0
24	509.0
25	1245.0
26	2752.0
27	5130.0
28	9096.0
29	14412.0
30	21499.0
31	30875.0
32	44147.0
33	69956.0
34	170447.0
35	405479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86078922501976	18.48262846402646	12.308646256460102	26.34793605449368
2	15.236252595704311	21.27040310595114	37.334574201373805	26.158770096970745
3	17.35599722093681	25.748742267669154	29.55242388189755	27.34283662949648
4	11.864170827973261	16.691716142604133	36.60012013436487	34.84399289505774
5	13.611738061693657	37.54828880086517	33.67899757798714	15.160975559454034
6	32.807940190770815	36.60595514307811	17.15687032740397	13.4292343387471
7	28.68222749853698	31.31952554415218	21.877586060217016	18.120660897093817
8	27.763354980419656	33.179724096326524	19.89899172185857	19.157929201395245
9	27.744936025182593	13.715076813608087	18.70377197482772	39.8362151863816
10	15.32732398287683	27.31882684898381	32.48877597187902	24.865073196260333
11	36.748852420133474	21.5212635817898	22.39794885809197	19.33193513998476
12	23.95884490401484	24.54740093091363	29.353684017855187	22.140070147216345
13	29.764333894903462	19.940422866173154	25.30429839610931	24.990944842814073
14	23.472385315306372	19.769631645228607	26.110561858002246	30.647421181462768
15	24.975734756722407	26.992360154214808	23.389374336975592	24.642530752087197
16	25.00399587522557	25.95063160608404	24.292987883475124	24.75238463521526
17	23.48965778595284	25.98076053008439	25.981147227188416	24.548434456774356
18	24.493781266072098	24.79785408887073	27.046755546847713	23.661609098209464
19	24.951243940134134	25.537218951767272	26.36178607658407	23.149751031514526
20	25.28767042063622	24.217292836693947	26.37171130225406	24.123325440415776
21	26.558614902532995	24.473801915697454	25.181586515098587	23.785996666670965
22	25.252223186100558	24.636601396492143	25.61159369477482	24.49958172263248
23	23.93210096945214	24.318154573542888	26.170051778875713	25.579692678129256
24	24.176174044632578	25.451629992742987	25.913088536879947	24.459107425744488
25	24.62541892240268	24.44508894044857	26.050657385924207	24.878834751224545
26	24.251740139211137	25.147718484145397	26.50657385924207	24.093967517401392
27	24.992233833160824	25.084525541988217	25.510021255450816	24.413219369400142
28	23.966842012320168	24.604376637823357	26.569958017584405	24.858823332272063
29	24.01453465514997	25.05642555242904	26.640852486655724	24.288187305765266
30	24.333044169832213	25.05230078331943	26.611205708680448	24.003449338167908
31	24.344516183918298	24.980632920040062	25.741652820762024	24.933198075279613
32	24.070090139094948	25.28161216600649	25.642013866958152	25.00628382794041
33	23.81783472823572	24.712394028881118	26.201564576482888	25.26820666640028
34	24.417601936579096	24.873388923190355	26.51723831240228	24.19177082782827
35	25.283545651526616	24.719612374822926	26.155032024965163	23.841809948685295
36	24.322861146092876	25.47264053539503	25.73572346516697	24.468774853345124
37	25.154388818782138	25.3188639870276	25.699373937388582	23.82737325680168
38	24.232631821820284	25.122937454321402	26.00834492350487	24.63608580035344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	215.0
1	192.5
2	170.0
3	170.0
4	566.0
5	962.0
6	962.0
7	1092.0
8	1222.0
9	1191.0
10	1160.0
11	1160.0
12	1540.0
13	1920.0
14	2340.5
15	2761.0
16	2761.0
17	4248.5
18	5736.0
19	5736.0
20	6417.5
21	7099.0
22	7412.5
23	7726.0
24	7726.0
25	8491.0
26	9256.0
27	9256.0
28	11429.5
29	13603.0
30	16271.0
31	18939.0
32	18939.0
33	23206.5
34	27474.0
35	27474.0
36	30451.5
37	33429.0
38	37881.5
39	42334.0
40	42334.0
41	46062.5
42	49791.0
43	55880.5
44	61970.0
45	61970.0
46	65945.0
47	69920.0
48	69920.0
49	71971.5
50	74023.0
51	72666.0
52	71309.0
53	71309.0
54	67639.0
55	63969.0
56	63969.0
57	61695.0
58	59421.0
59	52887.5
60	46354.0
61	46354.0
62	43133.5
63	39913.0
64	32715.5
65	25518.0
66	25518.0
67	21028.0
68	16538.0
69	16538.0
70	13162.0
71	9786.0
72	7569.5
73	5353.0
74	5353.0
75	3943.0
76	2533.0
77	2533.0
78	2441.0
79	2349.0
80	1803.0
81	1257.0
82	1257.0
83	1225.0
84	1193.0
85	1193.0
86	765.5
87	338.0
88	255.5
89	173.0
90	173.0
91	111.5
92	50.0
93	32.0
94	14.0
95	14.0
96	12.5
97	11.0
98	11.0
99	11.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010054124704660087
2	0.0
3	0.0
4	3.86697104025388E-4
5	0.0
6	1.2889903467512933E-4
7	9.022932427259052E-4
8	0.002964677797527974
9	0.0034802739362284913
10	0.001288990346751293
11	0.004898163317654914
12	5.155961387005173E-4
13	0.0
14	0.0
15	0.0
16	1.2889903467512933E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.5779806935025866E-4
24	0.0
25	1.2889903467512933E-4
26	1.2889903467512933E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	775801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.63350075038487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84232771943428	48.209110107197446
2	12.232083951804348	14.588839750373909
3	3.2438641785491975	5.803289307769806
4	1.2707096937169424	3.031074698951625
5	0.6405038829425168	1.9097744392038503
6	0.3800745235674506	1.3599104631817043
7	0.25991071416890793	1.084957003789725
8	0.17552886770504497	0.8373920691202404
9	0.1312484661486013	0.704412495410353
>10	0.6668931622154971	7.4206143282952235
>50	0.07799338679166018	3.259461984298124
>100	0.07234397750634568	8.323799035610023
>500	0.005213980359332008	1.9574206943095283
>1k	0.001303495089833002	1.5099436224884208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3383	0.4360654343059625	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2753	0.354859042460631	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1584	0.20417607092540485	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1520	0.19592653270619656	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1363	0.17568938426220126	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.13560178447823604	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	808	0.10415042001750449	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	778	0.10028344897725061	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.288990346751293E-4
2	0.0	0.0	0.0	0.0	1.288990346751293E-4
3	0.0	0.0	0.0	0.0	1.288990346751293E-4
4	0.0	0.0	0.0	0.0	1.288990346751293E-4
5	0.0	0.0	0.0	0.0	1.288990346751293E-4
6	0.0	0.0	0.0	0.0	2.577980693502586E-4
7	0.0	0.0	0.0	0.0	2.577980693502586E-4
8	0.0	0.0	0.0	0.0	2.577980693502586E-4
9	0.0	0.0	0.0	0.0	2.577980693502586E-4
10	0.0	0.0	0.0	1.288990346751293E-4	2.577980693502586E-4
11	0.0	0.0	0.0	1.288990346751293E-4	2.577980693502586E-4
12	0.0	0.0	0.0	1.288990346751293E-4	3.8669710402538794E-4
13	0.0	0.0	0.0	1.288990346751293E-4	9.022932427259052E-4
14	0.0	0.0	0.0	1.288990346751293E-4	9.022932427259052E-4
15	0.0	0.0	0.0	1.288990346751293E-4	0.0011600913120761638
16	0.0	0.0	0.0	1.288990346751293E-4	0.0011600913120761638
17	0.0	0.0	0.0	1.288990346751293E-4	0.0011600913120761638
18	0.0	0.0	0.0	2.577980693502586E-4	0.0011600913120761638
19	0.0	0.0	0.0	2.577980693502586E-4	0.0011600913120761638
20	0.0	0.0	0.0	2.577980693502586E-4	0.0014178893814264224
21	0.0	0.0	0.0	3.8669710402538794E-4	0.0015467884161015518
22	0.0	0.0	0.0	7.733942080507759E-4	0.0015467884161015518
23	0.0	0.0	0.0	0.0015467884161015518	0.0015467884161015518
24	0.0	0.0	0.0	0.002835778762852845	0.0015467884161015518
25	0.0	0.0	0.0	0.003609172970903621	0.0015467884161015518
26	0.0	0.0	0.0	0.004511466213629526	0.0015467884161015518
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	130	0.0	19.691673	28
CTAGCGG	140	1.8189894E-12	18.285126	29
CTAAGAC	85	4.9812497E-6	16.940632	3
AAGCTCG	210	0.0	16.761364	24
CGCTTCG	155	1.0913936E-11	16.515598	32
CGAGCCG	175	0.0	16.456612	15
TTTCGTT	235	0.0	16.3399	28
CTAGACA	120	3.10647E-8	15.999484	4
CTTTCGT	240	0.0	15.999484	27
CGACTTT	255	0.0	15.685769	24
TAGAGTG	195	0.0	15.59125	5
TAAACGC	165	2.910383E-11	15.514651	28
AACGCTT	135	7.898052E-9	15.406911	30
AGGCCCG	210	0.0	15.239568	10
CGCGCAA	105	2.1833384E-6	15.237604	21
TAGCGGC	170	4.7293724E-11	15.058339	30
TCGTTCT	255	0.0	15.058338	30
TCCGACT	255	0.0	15.058338	22
TTCGTTC	255	0.0	15.058338	29
CCGACTT	255	0.0	15.058338	23
>>END_MODULE