FastQCFastQC Report
Thu 2 Feb 2017
SRR4063062_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063062_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926836
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA78010.8416807288452326No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA75540.8150309224069846No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG64220.6928949673944473No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC61950.6684030400200252No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA40870.44096258669279137No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA33830.3650052436461251No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA29840.32195555632280143No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA28180.3040451600930477No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA26070.28127953596968613No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT24420.2634770336931237No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG23970.25862180579951577No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA21890.23617986353572798No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT18920.20413535943791566No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT18020.1944249036506998No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT17170.18525391762944038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT17060.18406708414433623No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG16800.18126184136136275No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG16500.17802502276562412No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA15920.17176717348052944No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT15610.16842246093159954No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT15550.16777509721245182No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA15390.16604879396139122No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA14740.15903568700395757No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC14600.15752517165927954No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA13350.1440384275103686No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT13270.1431752758848383No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA13220.14263580611888188No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA12750.13756479031889138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12670.13670163869336108No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA12520.13508322939549178No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT11840.12774644057848422No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT11780.1270990768593365No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT11660.12580434942104105No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11190.12073333362105056No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT11050.11922281827637252No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA10610.11447548433595589No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC10470.11296496899127785No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG10440.11264128713170399No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA10260.11069919597426081No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT10060.10854131691043506No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATG9660.10422555878278358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGC356.4326494E-527.426497
TTCTCCG308.419354E-426.66464427
CCGTCGT350.002066503322.8566429
GTCGTAG350.002066503322.85664211
ACCGTCG350.002067151722.8554088
GCGCAAG502.7764061E-522.4212861
TCATCGC502.7973263E-522.3995129
TAGGACC17050.021.579614
GCGAAAG507.195561E-419.1995818
CACGATT507.195561E-419.1995828
GCTACTA507.195561E-419.1995829
AGACGGA507.203403E-419.196476
CTAGGAC2550.018.1937163
AGGACCT36200.017.8972635
ATTGGCG450.00885013417.77738816
CGTCGTA450.00885013417.77738810
CGAACCT450.00885288617.77642827
TACCGTC450.00885288617.7764287
ATACCGT450.00885839117.774516
GGACCTG35900.017.6012216
Produced by FastQC (version 0.11.5)