FastQCFastQC Report
Thu 2 Feb 2017
SRR4063062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926836
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA80770.8714594599260279No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA71000.7660470676581402No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG67460.7278526082284245No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC60320.6508163256498453No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA42960.46351242290977046No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA35640.3845340491737481No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA26050.28106374806330353No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT25960.28009270248458196No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA25850.2789058689994778No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA25760.2779348234207562No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG24640.26585070066333205No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23980.2587296997527071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21340.2302456961102072No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT20100.21686684591448757No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT19460.20996163291024517No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG18790.2027327380464289No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG17870.19280649435283048No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT16640.17953553811030215No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA16460.17759344695285897No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT16440.1773776590464764No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT16230.17511188602945937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15870.17122770371457302No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA15500.1672356274464954No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA15360.16572511210181737No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA14900.16076199025501814No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC14810.15979094467629656No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA14070.15180679214014128No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT13930.15029627679546326No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA13630.14705945819972466No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA13090.14123318472739516No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA12780.13788847217846523No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT12740.13745689636570008No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT12240.1320621987061357No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11960.12904116801677967No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT11800.12731486476571907No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11590.12504909174870205No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT11530.1244017280295543No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA11040.11911492432318124No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC10910.11771230293169449No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT10180.10983604434873052No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG9880.1065992257529919No Hit
GTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGC9870.10649133179980062No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATG9460.10206767971895783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGTT200.003754228631.99811227
CGCGCGC308.418526E-426.66509422
CCCTCGA350.002066949622.85579326
TAGGGTC453.5310228E-421.337834
CCTCGCA453.5374647E-421.33207530
GACCTAC453.5374647E-421.3320751
TAGGACC16350.020.652674
TAACCTA555.8222773E-520.3679285
CCGCCCC400.004477370520.0042154
CCCGCCC400.004477370520.0042153
TCGCAAT400.00448436719.9988232
GTGTAGG1600.019.998821
TTGCGCT400.00448436719.9988212
CAACTAG400.00448436719.998821
GCTCGTT400.00448436719.9988212
GCGCTGT400.00448436719.9988214
GCGAAGA1300.019.69114720
CGAAGAA1400.019.42742321
GACCGCG751.5048809E-619.2040487
TCCGTTA507.1973575E-419.19886626
Produced by FastQC (version 0.11.5)