##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	926836
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31226991614482	32.0	32.0	32.0	32.0	32.0
2	31.454953195603107	32.0	32.0	32.0	32.0	32.0
3	31.498869271370555	32.0	32.0	32.0	32.0	32.0
4	31.57031880505289	32.0	32.0	32.0	32.0	32.0
5	31.54207648386554	32.0	32.0	32.0	32.0	32.0
6	35.0889952483503	36.0	36.0	36.0	36.0	36.0
7	35.128747696464096	36.0	36.0	36.0	36.0	36.0
8	35.04005023542461	36.0	36.0	36.0	36.0	36.0
9	35.145507943152836	36.0	36.0	36.0	36.0	36.0
10	35.04682921250362	36.0	36.0	36.0	36.0	36.0
11	35.1613802226068	36.0	36.0	36.0	36.0	36.0
12	35.12208848167313	36.0	36.0	36.0	36.0	36.0
13	35.134133762607405	36.0	36.0	36.0	36.0	36.0
14	35.10569075866712	36.0	36.0	36.0	36.0	36.0
15	35.07028751580646	36.0	36.0	36.0	36.0	36.0
16	35.10854455372903	36.0	36.0	36.0	36.0	36.0
17	35.12257616234156	36.0	36.0	36.0	36.0	36.0
18	35.12268513523428	36.0	36.0	36.0	36.0	36.0
19	35.06106258280861	36.0	36.0	36.0	36.0	36.0
20	35.04492704210885	36.0	36.0	36.0	36.0	36.0
21	34.99825643371643	36.0	36.0	36.0	36.0	36.0
22	34.976601038371406	36.0	36.0	36.0	36.0	36.0
23	34.955165746690895	36.0	36.0	36.0	36.0	36.0
24	34.94384767100112	36.0	36.0	36.0	36.0	36.0
25	34.90333133369874	36.0	36.0	36.0	36.0	36.0
26	34.85893297196052	36.0	36.0	36.0	36.0	36.0
27	34.77876237004173	36.0	36.0	36.0	32.0	36.0
28	34.70342757510498	36.0	36.0	36.0	32.0	36.0
29	34.6680437531559	36.0	36.0	36.0	32.0	36.0
30	34.5813239882784	36.0	36.0	36.0	32.0	36.0
31	34.57564984528007	36.0	36.0	36.0	32.0	36.0
32	34.543232028104214	36.0	36.0	36.0	32.0	36.0
33	34.6083913443155	36.0	36.0	36.0	32.0	36.0
34	34.55824007699312	36.0	36.0	36.0	32.0	36.0
35	34.54334207993647	36.0	36.0	36.0	32.0	36.0
36	34.45176385034677	36.0	36.0	36.0	32.0	36.0
37	34.34214683072302	36.0	36.0	36.0	32.0	36.0
38	33.9839475376442	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	10.0
22	42.0
23	128.0
24	382.0
25	950.0
26	2247.0
27	4567.0
28	8763.0
29	14392.0
30	23063.0
31	33781.0
32	48574.0
33	77000.0
34	200110.0
35	512820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.175216349785266	21.84312752228241	13.302112782441677	26.679543345490647
2	9.642051020892586	21.06187070851801	50.908359191917455	18.387719078671953
3	19.218286730338484	26.683253563737274	31.383761528468902	22.714698177455343
4	10.110526565649154	18.635012019386384	41.348091787543865	29.9063696274206
5	8.892511728072712	42.25332205481876	37.501780250227654	11.352385966880872
6	29.05907841300942	40.09091144495898	19.26112062975543	11.588889512276173
7	25.17988638754471	32.33703949462418	23.643474836405616	18.839599281425496
8	19.97952124158677	48.30580893253663	19.91154643758079	11.803123388295818
9	25.829364454236863	14.89176667091069	21.740861507519472	37.53800736733297
10	15.384773078670161	30.172342172527756	36.749462408517736	17.69342234028435
11	33.16655678908575	18.629051488526514	32.112163610055795	16.092228112331934
12	26.91166506264323	22.331243067813507	36.54940032540816	14.2076915441351
13	31.984691972888353	23.805125836989145	26.174697950226257	18.03548423989625
14	16.907629828793873	28.162803343849397	27.126374029493896	27.803192797862835
15	17.986569360706746	44.60260499160585	21.478772943649147	15.932052704038254
16	15.720041086017375	27.743959017560822	38.87268081947615	17.66331907694565
17	16.641994916036925	33.728512919221956	32.82241950032152	16.807072664419596
18	17.290653362622947	26.55550712315879	42.12762559935091	14.026213914867355
19	24.94616091735755	24.676102352519756	27.900189461781803	22.477547268340896
20	24.065098895597494	30.665619376027692	30.85939691595924	14.409884812415575
21	19.47856897783206	27.03612513136117	27.037851438337395	26.447454452469376
22	22.60464634520023	35.52634986124838	27.37744325857002	14.491560534981376
23	16.64751546931223	34.73196415759008	31.69312768723667	16.927392685861022
24	24.340012688328898	27.80265332809688	32.60501318464108	15.252320798933145
25	20.054270717010038	31.612099240965218	32.535780370831915	15.797849671192823
26	13.80621814431032	34.47535486321205	34.97371703300261	16.74470995947503
27	16.6769705503137	33.42310119924258	32.205948200057186	17.693980050386532
28	15.413514365000927	34.67992179846273	28.5318006637636	21.374763172772745
29	19.4558692152657	29.177438079660263	27.56237349433988	23.804319210734153
30	15.72705419297481	30.489968020232272	31.826558312365943	21.956419474426976
31	22.618025195395948	26.94597533975806	30.774700162704082	19.66129930214191
32	17.828936295094277	33.27578989163131	31.095684673448158	17.799589139826246
33	15.564565899468729	33.717615629949634	35.36321420402315	15.354604266558486
34	18.188223159221266	32.83471941098533	34.1056022856255	14.8714551441679
35	16.259187170114238	34.60461181913521	32.85306138302785	16.283139627722704
36	14.598159760734369	32.41511982702441	35.02119037240677	17.965530039834448
37	16.04588082465506	32.400338355437206	28.36175979353413	23.1920210263736
38	18.503549183078288	36.4531690175037	28.08402376695694	16.95925803246108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	133.0
2	100.0
3	100.0
4	320.5
5	541.0
6	541.0
7	649.5
8	758.0
9	757.0
10	756.0
11	756.0
12	999.0
13	1242.0
14	1669.0
15	2096.0
16	2096.0
17	3110.0
18	4124.0
19	4124.0
20	5239.0
21	6354.0
22	8830.0
23	11306.0
24	11306.0
25	19423.5
26	27541.0
27	27541.0
28	39829.0
29	52117.0
30	73537.5
31	94958.0
32	94958.0
33	117745.0
34	140532.0
35	140532.0
36	143958.5
37	147385.0
38	145883.0
39	144381.0
40	144381.0
41	119853.5
42	95326.0
43	74552.5
44	53779.0
45	53779.0
46	47012.0
47	40245.0
48	40245.0
49	34957.0
50	29669.0
51	25563.0
52	21457.0
53	21457.0
54	18513.5
55	15570.0
56	15570.0
57	13791.5
58	12013.0
59	10056.5
60	8100.0
61	8100.0
62	7317.5
63	6535.0
64	5182.0
65	3829.0
66	3829.0
67	3205.0
68	2581.0
69	2581.0
70	2006.5
71	1432.0
72	1132.0
73	832.0
74	832.0
75	594.0
76	356.0
77	356.0
78	353.0
79	350.0
80	262.5
81	175.0
82	175.0
83	157.0
84	139.0
85	139.0
86	92.0
87	45.0
88	34.0
89	23.0
90	23.0
91	17.5
92	12.0
93	8.5
94	5.0
95	5.0
96	4.5
97	4.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010357819506363585
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0789395319128735E-4
8	0.0023736669702083216
9	0.004531546034034068
10	0.001834197204251885
11	0.005071015799990505
12	0.0
13	2.157879063825747E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.157879063825747E-4
22	0.0
23	1.0789395319128735E-4
24	0.0
25	1.0789395319128735E-4
26	0.0
27	1.0789395319128735E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.2368185957386205E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	926836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.1559544937526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.44276422940275	38.40511283847139
2	10.833338981189211	10.217128800247092
3	2.9095364362453293	4.116059033564996
4	1.274857035558727	2.404684014193907
5	0.7180095924882117	1.6929213834725985
6	0.4845913535816075	1.3710820690536156
7	0.34410761819868313	1.1358706229311488
8	0.2516165390395593	0.9492174451859988
9	0.2121964051443674	0.9005691622253114
>10	1.2719217933391966	11.72272299953711
>50	0.14059099257232424	4.625292542225772
>100	0.09671293645992945	9.078175614217566
>500	0.010567209208015639	3.3971526943190673
>1k	0.00826998981496876	6.955713472647554
>5k	9.18887757218751E-4	3.028297307706936
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	8077	0.8714594599260279	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	7100	0.7660470676581402	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	6746	0.7278526082284245	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	6032	0.6508163256498453	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	4296	0.46351242290977046	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	3564	0.3845340491737481	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	2605	0.28106374806330353	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	2596	0.28009270248458196	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	2585	0.2789058689994778	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	2576	0.2779348234207562	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	2464	0.26585070066333205	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2398	0.2587296997527071	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2134	0.2302456961102072	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	2010	0.21686684591448757	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	1946	0.20996163291024517	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	1879	0.2027327380464289	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	1787	0.19280649435283048	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	1664	0.17953553811030215	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	1646	0.17759344695285897	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	1644	0.1773776590464764	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	1623	0.17511188602945937	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1587	0.17122770371457302	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	1550	0.1672356274464954	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	1536	0.16572511210181737	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	1490	0.16076199025501814	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	1481	0.15979094467629656	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1407	0.15180679214014128	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	1393	0.15029627679546326	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	1363	0.14705945819972466	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	1309	0.14123318472739516	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	1278	0.13788847217846523	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	1274	0.13745689636570008	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	1224	0.1320621987061357	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1196	0.12904116801677967	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	1180	0.12731486476571907	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1159	0.12504909174870205	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	1153	0.1244017280295543	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	1104	0.11911492432318124	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	1091	0.11771230293169449	No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT	1018	0.10983604434873052	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	988	0.1065992257529919	No Hit
GTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGC	987	0.10649133179980062	No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATG	946	0.10206767971895783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.0789395319128735E-4	0.0
6	0.0	0.0	0.0	2.157879063825747E-4	0.0
7	0.0	0.0	0.0	2.157879063825747E-4	0.0
8	0.0	0.0	0.0	2.157879063825747E-4	0.0
9	0.0	0.0	0.0	3.2368185957386205E-4	0.0
10	0.0	0.0	0.0	3.2368185957386205E-4	0.0
11	0.0	0.0	0.0	3.2368185957386205E-4	0.0
12	0.0	0.0	0.0	3.2368185957386205E-4	0.0
13	0.0	0.0	0.0	3.2368185957386205E-4	0.0
14	0.0	0.0	0.0	3.2368185957386205E-4	0.0
15	0.0	1.0789395319128735E-4	0.0	3.2368185957386205E-4	0.0
16	0.0	1.0789395319128735E-4	0.0	3.2368185957386205E-4	0.0
17	0.0	1.0789395319128735E-4	0.0	3.2368185957386205E-4	0.0
18	0.0	1.0789395319128735E-4	0.0	3.2368185957386205E-4	0.0
19	0.0	1.0789395319128735E-4	0.0	3.2368185957386205E-4	0.0
20	0.0	1.0789395319128735E-4	0.0	6.473637191477241E-4	0.0
21	0.0	1.0789395319128735E-4	0.0	6.473637191477241E-4	0.0
22	0.0	1.0789395319128735E-4	0.0	0.0010789395319128735	0.0
23	0.0	1.0789395319128735E-4	0.0	0.0019420911574431722	0.0
24	0.0	1.0789395319128735E-4	0.0	0.0029131367361647583	0.0
25	0.0	1.0789395319128735E-4	0.0	0.0034526065021211953	0.0
26	0.0	1.0789395319128735E-4	0.0	0.004315758127651494	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGTT	20	0.0037542286	31.998112	27
CGCGCGC	30	8.418526E-4	26.665094	22
CCCTCGA	35	0.0020669496	22.855793	26
TAGGGTC	45	3.5310228E-4	21.33783	4
CCTCGCA	45	3.5374647E-4	21.332075	30
GACCTAC	45	3.5374647E-4	21.332075	1
TAGGACC	1635	0.0	20.65267	4
TAACCTA	55	5.8222773E-5	20.367928	5
CCGCCCC	40	0.0044773705	20.004215	4
CCCGCCC	40	0.0044773705	20.004215	3
TCGCAAT	40	0.004484367	19.99882	32
GTGTAGG	160	0.0	19.99882	1
TTGCGCT	40	0.004484367	19.99882	12
CAACTAG	40	0.004484367	19.99882	1
GCTCGTT	40	0.004484367	19.99882	12
GCGCTGT	40	0.004484367	19.99882	14
GCGAAGA	130	0.0	19.691147	20
CGAAGAA	140	0.0	19.427423	21
GACCGCG	75	1.5048809E-6	19.204048	7
TCCGTTA	50	7.1973575E-4	19.198866	26
>>END_MODULE