FastQCFastQC Report
Thu 2 Feb 2017
SRR4063061_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063061_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727094
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45490.26339041187103884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29600.17138615500951307No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23490.13600881017477914No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21340.12356015364537193No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18680.10815855998573326No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18480.10700054542485817No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18460.10688474396877065No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18250.10566882867985182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17950.1039318068385392No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17650.10219478499722656No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17520.10144207553265776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG400.004490062619.995624
TCTAGCG4600.019.47737928
CTAGCGG4600.019.12956829
TAGCGGC4900.017.95837230
ACTCGTA450.00884990317.77851913
GTCCTAT4750.017.1936051
AACCGCG1850.016.4307377
ACCGTCG2900.015.998358
TACCGTC3150.015.7444087
GAACCGC2300.015.3014396
ATACCGT3300.015.0274476
GTATCAA31050.015.0079621
AGCGGCG6150.014.82859731
CCGTCGT3050.014.68913659
AAGACGG5400.014.5153395
TTATCCG901.5358583E-414.22116627
TAGGTTG1258.618081E-714.0769175
CGCTTCG4250.013.928781532
CAAGACG5200.013.8431224
CGTCGTA3250.013.7851910
Produced by FastQC (version 0.11.5)