##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063061_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1727094
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24760146234079	32.0	32.0	32.0	32.0	32.0
2	30.88214596310334	32.0	32.0	32.0	32.0	32.0
3	30.92577647771343	32.0	32.0	32.0	32.0	32.0
4	30.98634295527632	32.0	32.0	32.0	32.0	32.0
5	30.91111311833635	32.0	32.0	32.0	32.0	32.0
6	34.5539837437916	36.0	36.0	36.0	32.0	36.0
7	34.49037689899913	36.0	36.0	36.0	32.0	36.0
8	34.474008363181156	36.0	36.0	36.0	32.0	36.0
9	34.60384553475375	36.0	36.0	36.0	32.0	36.0
10	34.33550287361313	36.0	36.0	36.0	32.0	36.0
11	34.586705182230965	36.0	36.0	36.0	32.0	36.0
12	34.41714521618395	36.0	36.0	36.0	32.0	36.0
13	34.49366044928649	36.0	36.0	36.0	32.0	36.0
14	34.39663040923077	36.0	36.0	36.0	32.0	36.0
15	34.34975455881382	36.0	36.0	36.0	32.0	36.0
16	34.36622847395683	36.0	36.0	36.0	32.0	36.0
17	34.29472860191744	36.0	36.0	36.0	32.0	36.0
18	34.31445827499835	36.0	36.0	36.0	32.0	36.0
19	34.310783315789415	36.0	36.0	36.0	32.0	36.0
20	34.292917467144235	36.0	36.0	36.0	32.0	36.0
21	34.280286423321485	36.0	36.0	36.0	32.0	36.0
22	34.250399225519864	36.0	36.0	36.0	32.0	36.0
23	34.19986752313424	36.0	36.0	36.0	32.0	36.0
24	34.186182686061095	36.0	36.0	36.0	32.0	36.0
25	34.163262682864975	36.0	36.0	36.0	32.0	36.0
26	34.11559417148111	36.0	36.0	36.0	32.0	36.0
27	34.12729648762603	36.0	36.0	36.0	32.0	36.0
28	34.1126377603072	36.0	36.0	36.0	32.0	36.0
29	34.07825804501666	36.0	36.0	36.0	32.0	36.0
30	34.04698644080751	36.0	36.0	36.0	32.0	36.0
31	34.062293077273154	36.0	36.0	36.0	32.0	36.0
32	34.02847847308832	36.0	36.0	36.0	32.0	36.0
33	33.98345428795422	36.0	36.0	36.0	32.0	36.0
34	33.99977650318975	36.0	36.0	36.0	32.0	36.0
35	33.95954707734495	36.0	36.0	36.0	32.0	36.0
36	33.91390914449358	36.0	36.0	36.0	32.0	36.0
37	33.91143099333331	36.0	36.0	36.0	32.0	36.0
38	33.333099993399316	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	8.0
10	11.0
11	17.0
12	10.0
13	5.0
14	121.0
15	352.0
16	497.0
17	595.0
18	748.0
19	970.0
20	1386.0
21	2040.0
22	3133.0
23	4697.0
24	7127.0
25	11046.0
26	16246.0
27	23456.0
28	33039.0
29	45072.0
30	60814.0
31	81542.0
32	111421.0
33	166372.0
34	368682.0
35	787678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.23811456165471	17.31056631555759	11.482252276798395	25.969066845989296
2	16.906481280324424	19.380230284597	37.220189731334635	26.493098703743932
3	18.872461022100023	23.166921075977662	28.69007386749251	29.2705440344298
4	12.716029009423721	15.03741984047712	35.98048667506261	36.26606447503655
5	14.728126501803612	36.296023947796584	33.67056725474642	15.305282295653383
6	34.59939725028009	35.38279818306568	16.53242312914535	13.485381437508867
7	30.56156758115077	30.39319226399953	20.673165444382295	18.37207471046741
8	28.35937907115437	32.72516197766185	19.346299266396077	19.569159684787707
9	27.316816147392437	14.096428095814348	18.7391508259558	39.84760493083742
10	15.990701094618966	26.318913081869162	31.45230257920137	26.238083244310502
11	37.752481095040125	21.140435190439245	21.967852882934004	19.139230831586627
12	24.541731951551956	23.71841861768592	28.525412756811342	23.214436673950782
13	29.40388568781307	19.161363175724585	25.27735472102383	26.157396415438516
14	23.5292039846225	19.631382463422277	25.04662874600666	31.79278480594856
15	25.262797610946603	26.947852822913415	22.352521472929137	25.436828093210845
16	25.880799687305473	25.82439885924403	23.571492682079416	24.723308771371077
17	24.18733592817527	25.891279558988835	25.169986083755752	24.751398429080144
18	25.212363216085294	24.535696128648972	26.050756401006602	24.201184254259132
19	25.438166775527975	24.96948409003525	25.619351371884136	23.972997762552637
20	25.725125245221225	24.134979953816185	25.15639729197018	24.98349750899241
21	26.914355596886587	24.14020329153006	24.589832644075376	24.355608467507984
22	25.725807105376227	24.11165425767846	25.195515392820045	24.967023244125272
23	24.282719723904712	23.933373481418	25.775881038136824	26.00802575654047
24	24.62046808550065	24.97562054684098	25.327877586190922	25.076033781467444
25	24.882124776902824	24.371071549768185	25.401169544586427	25.34563412874256
26	24.660683665154988	25.17371919195312	25.791284476722875	24.374312666169015
27	25.38821489765441	24.58511948570393	24.96023319820052	25.066432418441142
28	24.41848547683004	24.587796518700937	25.90592119624852	25.087796808220503
29	24.411649743326937	24.926269829550556	25.836279355262736	24.82580107185977
30	24.50781651244406	25.031079025551676	25.933832798027158	24.527271663977107
31	25.02006390338753	24.84299199412617	24.830079294399134	25.30686480808717
32	24.756960830580464	24.760840424207505	24.819497563673107	25.662701181538928
33	24.451852482377923	24.372058764772405	25.42611177026821	25.749976982581458
34	25.11128444866786	24.652248765259447	25.590993241572523	24.64547354450017
35	25.81720788151666	24.440519450436497	25.492739565387346	24.249533102659505
36	24.561296834527123	25.159379703286504	25.1547469932264	25.124576468959976
37	25.587879510830508	24.825658525230075	25.019471002538037	24.56699096140138
38	24.559333173131208	24.613242153232367	25.67439786680255	25.15302680683387
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	93.5
2	145.0
3	145.0
4	460.5
5	776.0
6	776.0
7	992.0
8	1208.0
9	1216.0
10	1224.0
11	1224.0
12	1687.0
13	2150.0
14	2998.0
15	3846.0
16	3846.0
17	5731.5
18	7617.0
19	7617.0
20	9407.0
21	11197.0
22	12370.5
23	13544.0
24	13544.0
25	15694.5
26	17845.0
27	17845.0
28	22411.0
29	26977.0
30	32621.0
31	38265.0
32	38265.0
33	47876.5
34	57488.0
35	57488.0
36	65060.0
37	72632.0
38	82839.5
39	93047.0
40	93047.0
41	100435.0
42	107823.0
43	121925.5
44	136028.0
45	136028.0
46	139926.0
47	143824.0
48	143824.0
49	151913.0
50	160002.0
51	162061.0
52	164120.0
53	164120.0
54	156558.0
55	148996.0
56	148996.0
57	143479.5
58	137963.0
59	123315.0
60	108667.0
61	108667.0
62	102719.0
63	96771.0
64	80555.5
65	64340.0
66	64340.0
67	53894.5
68	43449.0
69	43449.0
70	34817.0
71	26185.0
72	20543.0
73	14901.0
74	14901.0
75	11366.0
76	7831.0
77	7831.0
78	7298.0
79	6765.0
80	5237.0
81	3709.0
82	3709.0
83	3784.0
84	3859.0
85	3859.0
86	2556.0
87	1253.0
88	1083.0
89	913.0
90	913.0
91	651.0
92	389.0
93	354.5
94	320.0
95	320.0
96	291.0
97	262.0
98	262.0
99	491.5
100	721.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12066511724318423
2	0.05610580547439803
3	0.012622358713538465
4	0.003995150235019055
5	2.3160291217501767E-4
6	5.211065523937898E-4
7	0.0
8	2.3160291217501767E-4
9	2.3160291217501767E-4
10	5.211065523937898E-4
11	0.0019686247534876503
12	0.0027792349461002123
13	0.014127777642676078
14	0.008974612846781936
15	0.02229178029684545
16	0.009785223039394497
17	0.01939674389465773
18	0.005790072804375442
19	0.007295491733513057
20	0.004747859699587863
21	0.006716484453075513
22	0.006948087365250532
23	0.008916712118738181
24	0.013317167450063519
25	0.015517395115726185
26	0.018122927877695136
27	0.008743009934606917
28	0.0055005691641566695
29	0.010943237600269586
30	0.0026055327619689487
31	0.006484881540900495
32	0.006311179356769232
33	0.008164002654169373
34	0.01233285507331969
35	0.01557529584376994
36	0.014069876914632323
37	0.009669421583306989
38	0.006311179356769232
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1727094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.62283894584279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87410586331026	44.98447365323947
2	11.793490641310818	13.119748611018716
3	3.2676410207756073	5.452664106942928
4	1.3281509739200816	2.9550211087248384
5	0.6768253451539009	1.8823473583979944
6	0.40465360954921464	1.350478953168595
7	0.27187788315206796	1.0585833795252897
8	0.2034391387118597	0.9052689958280594
9	0.16473044634333261	0.8246497577788787
>10	0.8591283553772836	8.632464627598042
>50	0.07024618317158945	2.7419815688862164
>100	0.07097293484354907	8.5093637855252
>500	0.010243157654447377	3.7893259364977583
>1k	0.004494446725930992	3.7936281568681416
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4549	0.26339041187103884	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2960	0.17138615500951307	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2349	0.13600881017477914	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2134	0.12356015364537193	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1868	0.10815855998573326	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1848	0.10700054542485817	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1846	0.10688474396877065	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1825	0.10566882867985182	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1795	0.1039318068385392	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1765	0.10219478499722656	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1752	0.10144207553265776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.7900728043754425E-5
2	0.0	0.0	0.0	0.0	5.7900728043754425E-5
3	0.0	0.0	0.0	0.0	5.7900728043754425E-5
4	0.0	0.0	0.0	0.0	5.7900728043754425E-5
5	0.0	5.7900728043754425E-5	0.0	0.0	5.7900728043754425E-5
6	0.0	5.7900728043754425E-5	0.0	0.0	5.7900728043754425E-5
7	0.0	5.7900728043754425E-5	0.0	0.0	5.7900728043754425E-5
8	0.0	5.7900728043754425E-5	0.0	0.0	5.7900728043754425E-5
9	0.0	5.7900728043754425E-5	0.0	5.7900728043754425E-5	5.7900728043754425E-5
10	0.0	5.7900728043754425E-5	0.0	5.7900728043754425E-5	1.1580145608750885E-4
11	0.0	5.7900728043754425E-5	0.0	5.7900728043754425E-5	1.1580145608750885E-4
12	0.0	5.7900728043754425E-5	0.0	5.7900728043754425E-5	2.316029121750177E-4
13	0.0	5.7900728043754425E-5	0.0	5.7900728043754425E-5	4.632058243500354E-4
14	0.0	5.7900728043754425E-5	0.0	1.1580145608750885E-4	4.632058243500354E-4
15	0.0	5.7900728043754425E-5	0.0	1.1580145608750885E-4	4.632058243500354E-4
16	0.0	5.7900728043754425E-5	0.0	1.1580145608750885E-4	4.632058243500354E-4
17	0.0	5.7900728043754425E-5	0.0	1.1580145608750885E-4	5.211065523937898E-4
18	0.0	5.7900728043754425E-5	0.0	1.1580145608750885E-4	5.790072804375443E-4
19	0.0	5.7900728043754425E-5	0.0	1.7370218413126327E-4	5.790072804375443E-4
20	0.0	1.1580145608750885E-4	0.0	3.4740436826252654E-4	8.106101926125619E-4
21	0.0	1.1580145608750885E-4	0.0	4.0530509630628094E-4	8.685109206563163E-4
22	0.0	1.1580145608750885E-4	0.0	6.369080084812986E-4	9.843123767438251E-4
23	0.0	1.1580145608750885E-4	0.0	0.001100113832831334	9.843123767438251E-4
24	0.0	1.1580145608750885E-4	0.0	0.0016212203852251238	0.001100113832831334
25	0.0	1.1580145608750885E-4	0.0	0.0019107240254438958	0.001100113832831334
26	0.0	1.1580145608750885E-4	0.0	0.002026525481531405	0.0012738160169625972
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCCG	40	0.0044900626	19.99562	4
TCTAGCG	460	0.0	19.477379	28
CTAGCGG	460	0.0	19.129568	29
TAGCGGC	490	0.0	17.958372	30
ACTCGTA	45	0.008849903	17.778519	13
GTCCTAT	475	0.0	17.193605	1
AACCGCG	185	0.0	16.430737	7
ACCGTCG	290	0.0	15.99835	8
TACCGTC	315	0.0	15.744408	7
GAACCGC	230	0.0	15.301439	6
ATACCGT	330	0.0	15.027447	6
GTATCAA	3105	0.0	15.007962	1
AGCGGCG	615	0.0	14.828597	31
CCGTCGT	305	0.0	14.6891365	9
AAGACGG	540	0.0	14.515339	5
TTATCCG	90	1.5358583E-4	14.221166	27
TAGGTTG	125	8.618081E-7	14.076917	5
CGCTTCG	425	0.0	13.9287815	32
CAAGACG	520	0.0	13.843122	4
CGTCGTA	325	0.0	13.78519	10
>>END_MODULE