FastQCFastQC Report
Thu 2 Feb 2017
SRR4063061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727094
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38000.22002276656626682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25970.15036819072963023No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23160.13409808614933524No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22760.13178205702758505No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20530.11887019467382784No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20000.11580145608750886No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19620.11360122842184617No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19040.11024298619530842No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17700.10248428863744533No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17350.10045776315591391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3500.019.1990628
CTAGCGG3800.017.68334829
TAGCGGC4000.016.7991830
ACCGTCG4400.016.7298458
AACCGCT805.616089E-516.0024627
CGCGTAA2100.016.00014510
TACCGTC4850.015.8374877
ATACCGT4950.015.5175386
ATCGTTT3650.015.34171729
CGCTTCG4050.015.01161232
TCGCGTA2250.014.9351989
GCGTAAC2250.014.93346911
CGTTTAT3650.014.90338231
GTCGCGT2300.014.6109438
AAGACGG6050.014.5476925
TCTAGAT3750.014.5063792
GTATCAA31700.014.3862371
TTTTCGG4700.014.297173529
ATTGGAC901.5336397E-414.2235863
ATAACGA1801.1823431E-1014.22152712
Produced by FastQC (version 0.11.5)