##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063061_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1727094
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19301844601394	32.0	32.0	32.0	32.0	32.0
2	31.269697538176846	32.0	32.0	32.0	32.0	32.0
3	31.35785197563074	32.0	32.0	32.0	32.0	32.0
4	31.46012550561811	32.0	32.0	32.0	32.0	32.0
5	31.3810105298264	32.0	32.0	32.0	32.0	32.0
6	34.90684293964312	36.0	36.0	36.0	36.0	36.0
7	34.930901271152585	36.0	36.0	36.0	36.0	36.0
8	34.8588212338182	36.0	36.0	36.0	32.0	36.0
9	34.97471996312882	36.0	36.0	36.0	36.0	36.0
10	34.81382310401171	36.0	36.0	36.0	32.0	36.0
11	34.99027094066681	36.0	36.0	36.0	36.0	36.0
12	34.89288017907537	36.0	36.0	36.0	32.0	36.0
13	34.94639782200621	36.0	36.0	36.0	36.0	36.0
14	34.89478742905713	36.0	36.0	36.0	32.0	36.0
15	34.865457815266566	36.0	36.0	36.0	32.0	36.0
16	34.872540811328165	36.0	36.0	36.0	32.0	36.0
17	34.841169617866775	36.0	36.0	36.0	32.0	36.0
18	34.84176715338019	36.0	36.0	36.0	32.0	36.0
19	34.83265994786618	36.0	36.0	36.0	32.0	36.0
20	34.824682385556315	36.0	36.0	36.0	32.0	36.0
21	34.81891257800675	36.0	36.0	36.0	32.0	36.0
22	34.79783323895515	36.0	36.0	36.0	32.0	36.0
23	34.74065916504834	36.0	36.0	36.0	32.0	36.0
24	34.71425643306039	36.0	36.0	36.0	32.0	36.0
25	34.704499002370454	36.0	36.0	36.0	32.0	36.0
26	34.641376786671714	36.0	36.0	36.0	32.0	36.0
27	34.62563647375302	36.0	36.0	36.0	32.0	36.0
28	34.59591429302632	36.0	36.0	36.0	32.0	36.0
29	34.562060895353696	36.0	36.0	36.0	32.0	36.0
30	34.54534032310922	36.0	36.0	36.0	32.0	36.0
31	34.5398594401926	36.0	36.0	36.0	32.0	36.0
32	34.51211920138684	36.0	36.0	36.0	32.0	36.0
33	34.47533197382424	36.0	36.0	36.0	32.0	36.0
34	34.4694845792991	36.0	36.0	36.0	32.0	36.0
35	34.43657322647175	36.0	36.0	36.0	32.0	36.0
36	34.412975205750236	36.0	36.0	36.0	32.0	36.0
37	34.41073039452398	36.0	36.0	36.0	32.0	36.0
38	33.97486876799989	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	31.0
22	108.0
23	379.0
24	1036.0
25	2583.0
26	5675.0
27	11196.0
28	19961.0
29	31993.0
30	48962.0
31	71135.0
32	102493.0
33	163099.0
34	395973.0
35	872465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68546833443451	18.56324977315354	12.156827774461055	26.594454117950896
2	15.956282634297844	20.407980109941903	36.38979696530704	27.245940290453213
3	17.92855513365225	24.02330156899393	28.972250497077752	29.075892800276073
4	12.142961777926004	15.921222448614461	35.94749784464166	35.98831792881788
5	14.22111361628261	36.909166495859516	33.241097473559634	15.628622414298238
6	34.6334207443855	35.773894686376906	16.238016685880112	13.354667883357488
7	30.352067211515354	30.63143968015471	20.58746384804454	18.429029260285393
8	28.23346917221661	33.031430546725446	19.12734229429905	19.607757986758894
9	27.26275220985806	13.971699456988107	18.464952050741328	40.300596282412506
10	15.758435505117033	26.636191248208675	31.51669730613899	26.0886759405353
11	38.01523924008781	21.042867094115827	21.803960534780476	19.137933131015888
12	24.36165636126032	23.902443825933496	28.440258076171034	23.295641736635154
13	29.480768829917576	19.091571265456615	25.22517619211947	26.202483712506343
14	23.37035505884451	19.595748696944113	24.95405577229728	32.079840471914096
15	25.255371161036976	27.13998195813314	22.063246123256754	25.541400757573125
16	25.975744198919337	25.863358755642434	23.31977682717539	24.841120218262837
17	24.18368658567513	25.986715256957645	24.903682138899214	24.925916018468016
18	25.200133866483238	24.736870141405156	25.84671129654784	24.21628469556376
19	25.50324417779229	25.14292794717601	25.341064238541733	24.012763636489964
20	25.717303169370055	24.204588748498924	24.94664447910768	25.131463603023345
21	26.94162966325496	24.25028646401786	24.38924829178278	24.418835580944396
22	25.726219881488788	24.28547606557605	25.001186964924898	24.98711708801026
23	24.2570748981525	23.993105173320238	25.579007950937182	26.170811977590073
24	24.74404983168258	25.121852082168083	25.04814445536838	25.085953630780956
25	25.019830999354987	24.398845691085718	25.192143566013197	25.389179743546098
26	24.779803276258587	25.288982868312747	25.52417589798343	24.407037957445233
27	25.56878780193782	24.638554705186863	24.64312886270232	25.149528630172995
28	24.459873058443836	24.63282253311053	25.77091924353857	25.136385164907065
29	24.497740134584454	24.9561980992349	25.61470307927652	24.93135868690413
30	24.601845643607124	25.069509824016528	25.710412982732844	24.618231549643504
31	25.051213193954702	24.823663332742747	24.67370044710942	25.451423026193133
32	24.78701217189105	24.81080937111703	24.755629977291335	25.64654847970058
33	24.426695941274765	24.42663804054672	25.340022025436948	25.806643992741563
34	25.138295888932504	24.603408963264304	25.481589305503928	24.77670584229926
35	25.81393948447508	24.50972558528951	25.354497207447885	24.321837722787524
36	24.69680283759888	25.19665982280061	24.96656232955473	25.139975010045777
37	25.651527942312345	24.91763621435776	24.81121467621334	24.619621167116556
38	24.672556718138512	24.572793705955615	25.47384535748799	25.280804218417884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	53.5
2	85.0
3	85.0
4	300.5
5	516.0
6	516.0
7	721.5
8	927.0
9	1029.5
10	1132.0
11	1132.0
12	1494.0
13	1856.0
14	2682.5
15	3509.0
16	3509.0
17	5286.5
18	7064.0
19	7064.0
20	8710.5
21	10357.0
22	11947.5
23	13538.0
24	13538.0
25	15943.5
26	18349.0
27	18349.0
28	22833.0
29	27317.0
30	32745.5
31	38174.0
32	38174.0
33	48462.0
34	58750.0
35	58750.0
36	65636.5
37	72523.0
38	82718.5
39	92914.0
40	92914.0
41	100504.0
42	108094.0
43	122227.5
44	136361.0
45	136361.0
46	140005.0
47	143649.0
48	143649.0
49	152428.5
50	161208.0
51	162385.0
52	163562.0
53	163562.0
54	156143.5
55	148725.0
56	148725.0
57	143667.0
58	138609.0
59	124313.5
60	110018.0
61	110018.0
62	103934.5
63	97851.0
64	81080.5
65	64310.0
66	64310.0
67	53972.0
68	43634.0
69	43634.0
70	35180.5
71	26727.0
72	20682.5
73	14638.0
74	14638.0
75	11040.5
76	7443.0
77	7443.0
78	7124.0
79	6805.0
80	5180.0
81	3555.0
82	3555.0
83	3413.0
84	3271.0
85	3271.0
86	2090.0
87	909.0
88	723.0
89	537.0
90	537.0
91	310.0
92	83.0
93	59.5
94	36.0
95	36.0
96	27.5
97	19.0
98	19.0
99	18.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008974612846781936
2	0.0
3	0.0
4	1.7370218413126327E-4
5	0.0
6	2.8950364021877213E-4
7	5.211065523937898E-4
8	0.0027792349461002123
9	0.003474043682625266
10	0.001100113832831334
11	0.0060795764445942145
12	4.0530509630628094E-4
13	1.1580145608750884E-4
14	0.0
15	0.0
16	1.1580145608750884E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	5.790072804375442E-5
22	0.0
23	1.1580145608750884E-4
24	0.0
25	0.0
26	1.1580145608750884E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.790072804375442E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1727094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.9325208015991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62765358775589	42.94520083504171
2	12.484412503339088	13.466316740641584
3	3.502241520253757	5.666541409299294
4	1.4406399969578236	3.1078938641417393
5	0.7403737129078402	1.9965110336179623
6	0.4518893816078209	1.4622920084151332
7	0.2956050901533277	1.1159909371627046
8	0.21131275581611603	0.9117303678956726
9	0.16763664181620613	0.8136960004676478
>10	0.9110668923350378	8.85778160042172
>50	0.07489018012273767	2.8279251969800234
>100	0.07706039849906414	9.022474929415388
>500	0.01046866544835012	3.9334681772942206
>1k	0.004748672986880466	3.8721768992053334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3800	0.22002276656626682	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2597	0.15036819072963023	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2316	0.13409808614933524	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2276	0.13178205702758505	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2053	0.11887019467382784	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2000	0.11580145608750886	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1962	0.11360122842184617	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1904	0.11024298619530842	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1770	0.10248428863744533	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1735	0.10045776315591391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7370218413126327E-4
2	0.0	0.0	0.0	0.0	1.7370218413126327E-4
3	0.0	0.0	0.0	0.0	1.7370218413126327E-4
4	0.0	0.0	0.0	0.0	1.7370218413126327E-4
5	0.0	0.0	0.0	0.0	2.316029121750177E-4
6	0.0	0.0	0.0	0.0	2.316029121750177E-4
7	0.0	0.0	0.0	0.0	2.316029121750177E-4
8	0.0	0.0	0.0	0.0	2.316029121750177E-4
9	0.0	0.0	0.0	0.0	2.316029121750177E-4
10	0.0	0.0	0.0	0.0	2.316029121750177E-4
11	0.0	0.0	0.0	0.0	2.316029121750177E-4
12	0.0	0.0	0.0	0.0	2.8950364021877213E-4
13	0.0	0.0	0.0	0.0	2.8950364021877213E-4
14	0.0	0.0	0.0	5.7900728043754425E-5	3.4740436826252654E-4
15	0.0	0.0	0.0	5.7900728043754425E-5	3.4740436826252654E-4
16	0.0	0.0	0.0	5.7900728043754425E-5	3.4740436826252654E-4
17	0.0	0.0	0.0	5.7900728043754425E-5	3.4740436826252654E-4
18	0.0	0.0	0.0	5.7900728043754425E-5	3.4740436826252654E-4
19	0.0	0.0	0.0	5.7900728043754425E-5	4.0530509630628094E-4
20	0.0	0.0	0.0	1.7370218413126327E-4	6.948087365250531E-4
21	0.0	0.0	0.0	2.316029121750177E-4	6.948087365250531E-4
22	0.0	0.0	0.0	4.632058243500354E-4	6.948087365250531E-4
23	0.0	0.0	0.0	9.264116487000708E-4	7.527094645688075E-4
24	0.0	0.0	0.0	0.0014475182010938606	7.527094645688075E-4
25	0.0	0.0	0.0	0.0016791211132688782	7.527094645688075E-4
26	0.0	0.0	0.0	0.0017949225693563871	9.264116487000708E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	350	0.0	19.19906	28
CTAGCGG	380	0.0	17.683348	29
TAGCGGC	400	0.0	16.79918	30
ACCGTCG	440	0.0	16.729845	8
AACCGCT	80	5.616089E-5	16.002462	7
CGCGTAA	210	0.0	16.000145	10
TACCGTC	485	0.0	15.837487	7
ATACCGT	495	0.0	15.517538	6
ATCGTTT	365	0.0	15.341717	29
CGCTTCG	405	0.0	15.011612	32
TCGCGTA	225	0.0	14.935198	9
GCGTAAC	225	0.0	14.933469	11
CGTTTAT	365	0.0	14.903382	31
GTCGCGT	230	0.0	14.610943	8
AAGACGG	605	0.0	14.547692	5
TCTAGAT	375	0.0	14.506379	2
GTATCAA	3170	0.0	14.386237	1
TTTTCGG	470	0.0	14.2971735	29
ATTGGAC	90	1.5336397E-4	14.223586	3
ATAACGA	180	1.1823431E-10	14.221527	12
>>END_MODULE