FastQCFastQC Report
Thu 2 Feb 2017
SRR4063060_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063060_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328919
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30590.9300162045974845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23230.7062529072507212No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16060.48826610806915993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12080.36726367281914396No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT11770.3578388600232884No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11680.3551026240502981No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA11610.3529744405157501No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10860.33017247407416417No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA10390.3158832417707703No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT10340.3143631106746646No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG9510.28912893447930954No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9400.2857846460678769No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC8950.2721034662029253No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT7370.22406732356598436No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT7330.22285121868909977No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA7240.22011498271610944No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA6790.20643380285115787No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6790.20643380285115787No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC6780.20612977663193674No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC6650.20217743578206182No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6520.19822509493218696No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG6320.19214457054776404No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT6060.18423988884801426No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT6030.18332781019035083No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT5940.1805915742173605No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC5910.17967949555969706No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG5900.17937546934047593No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA5340.16235000106409178No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG5320.16174194862564947No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA5280.1605258437487649No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT5190.15778960777577458No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC5160.15687752911811115No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT5110.1553573980220054No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT4940.15018895229524595No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4870.14806076876069793No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC4810.14623661144537106No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC4770.14502050656848647No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA4750.14441245413004417No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA4710.14319634925315958No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA4680.14228427059549614No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT4670.141980244376275No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAA4590.13954803462250587No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG4590.13954803462250587No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG4440.13498764133418867No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG4360.1325554315804195No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA4310.13103530048431375No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT4290.13042724804587147No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG4280.13012322182665031No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG4170.12677893341521773No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG4130.12556282853833314No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA4110.12495477609989086No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC4080.12404269744222741No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4070.12373867122300627No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA4010.12191451390767939No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG4010.12191451390767939No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA3990.1213064614692371No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT3990.1213064614692371No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.1213064614692371No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3940.11978633037313137No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC3930.11948230415391023No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATAT3830.11644204196169879No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT3810.11583398952325649No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGG3800.11552996330403535No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC3780.11492191086559306No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA3770.1146178846463719No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3740.11370580598870847No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC3650.11096957001571815No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.11096957001571815No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT3630.11036151757727586No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA3540.10762528160428554No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3530.1073212553850644No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA3530.1073212553850644No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT3500.10640917672740098No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGT3470.10549709806973755No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3470.10549709806973755No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTAT3460.10519307185051639No Hit
GTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAG3400.10336891453518952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC252.8867213E-432.00731
CAAGTTT308.4002933E-426.66869528
TAAATTC401.58936E-424.00182316
TTGAACT350.00206251122.8588819
TCAAGTT350.00206251122.85888127
CGTATGT1150.022.26256223
GACGTGG1101.8189894E-1221.8165237
ATAGGAC453.538434E-421.3252263
CTACACT453.538434E-421.3252264
CTCGGAG1151.8189894E-1220.87115331
TAGGACC1700.020.6980114
CTGTAGG3500.020.5761221
TGTAGGA3500.020.5667362
TCAGGAC951.718945E-920.2028453
TCGGAGA1203.6379788E-1220.00152232
TGAACTT400.004474822420.0015210
ACGATTA801.2576311E-720.0015232
TCGTCCC400.004474822420.0015213
GCCGTTT400.004474822420.0015211
CTGTGCA400.004474822420.001529
Produced by FastQC (version 0.11.5)