##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063060_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328919
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011242889586796	32.0	32.0	32.0	32.0	32.0
2	30.74708362849212	32.0	32.0	32.0	32.0	32.0
3	30.83556133880986	32.0	32.0	32.0	32.0	32.0
4	30.857688367044773	32.0	32.0	32.0	32.0	32.0
5	30.863930025325384	32.0	32.0	32.0	32.0	32.0
6	34.43177803653787	36.0	36.0	36.0	32.0	36.0
7	34.2713342798683	36.0	36.0	36.0	32.0	36.0
8	34.23437685265977	36.0	36.0	36.0	32.0	36.0
9	34.35096178694451	36.0	36.0	36.0	32.0	36.0
10	34.16541154509165	36.0	36.0	36.0	32.0	36.0
11	34.47753094226846	36.0	36.0	36.0	32.0	36.0
12	34.288733700394324	36.0	36.0	36.0	32.0	36.0
13	34.36328700987173	36.0	36.0	36.0	32.0	36.0
14	34.28713756274341	36.0	36.0	36.0	32.0	36.0
15	34.26603510286727	36.0	36.0	36.0	32.0	36.0
16	34.26212532568809	36.0	36.0	36.0	32.0	36.0
17	34.23274119160036	36.0	36.0	36.0	32.0	36.0
18	34.262155728310006	36.0	36.0	36.0	32.0	36.0
19	34.213137580984984	36.0	36.0	36.0	32.0	36.0
20	34.14773850096832	36.0	36.0	36.0	32.0	36.0
21	34.076517318853575	36.0	36.0	36.0	32.0	36.0
22	34.06112447137441	36.0	36.0	36.0	32.0	36.0
23	34.041247237161734	36.0	36.0	36.0	32.0	36.0
24	34.007691863346295	36.0	36.0	36.0	32.0	36.0
25	33.99576795502844	36.0	36.0	36.0	32.0	36.0
26	33.99257567972662	36.0	36.0	36.0	32.0	36.0
27	33.974118247957705	36.0	36.0	36.0	32.0	36.0
28	33.94429327585211	36.0	36.0	36.0	32.0	36.0
29	33.87158236526318	36.0	36.0	36.0	32.0	36.0
30	33.80175970375685	36.0	36.0	36.0	32.0	36.0
31	33.81301779465461	36.0	36.0	36.0	32.0	36.0
32	33.7513460760856	36.0	36.0	36.0	27.0	36.0
33	33.69910221057464	36.0	36.0	36.0	27.0	36.0
34	33.66762941636087	36.0	36.0	36.0	27.0	36.0
35	33.55207817122149	36.0	36.0	36.0	21.0	36.0
36	33.49386019050283	36.0	36.0	36.0	21.0	36.0
37	33.45407531945555	36.0	36.0	36.0	21.0	36.0
38	32.790981974285465	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	2.0
12	1.0
13	3.0
14	71.0
15	118.0
16	177.0
17	222.0
18	298.0
19	388.0
20	496.0
21	646.0
22	915.0
23	1288.0
24	1589.0
25	2460.0
26	3432.0
27	4911.0
28	6739.0
29	9493.0
30	12789.0
31	16783.0
32	22253.0
33	32297.0
34	71193.0
35	140342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02377966369979	21.534415370940838	13.000280047242752	22.44152491811662
2	12.721028632960909	22.673666984033314	43.914925459395825	20.690378923609952
3	17.98495527325578	28.934950104291456	32.14882968566616	20.931264936786604
4	9.059593628671585	18.85586070395943	43.12047940020249	28.9640662671665
5	9.068798093147553	42.13620982673546	37.865553525336026	10.929438554780964
6	25.381479218161417	43.53864348756677	19.940896943605836	11.138980350665975
7	23.58452992986115	35.70179892313913	23.215746125945902	17.497925021053813
8	23.926936196863657	37.86141226688719	21.876577140807132	16.335074395442025
9	25.783551573487696	15.725452162994536	22.585195747281244	35.90580051623652
10	13.646216849741123	29.66110197343419	36.3727847889602	20.31989638786449
11	31.744024128474397	24.989510680863226	26.748979951718727	16.51748523894365
12	21.13904192053705	28.95764165055274	33.53217313048026	16.371143298429956
13	26.564029897038882	23.639094817887138	29.2964830232742	20.50039226179978
14	20.198491247594113	25.689231059258518	29.5787838154226	24.53349387772477
15	22.546908737037374	31.30280083933948	27.96186479335827	18.188425630264877
16	20.374672366924717	30.182075810188703	30.186940895297287	19.25631092758929
17	17.467499885964514	29.216804269488666	31.741093828399396	21.574602016147427
18	17.644841058696546	29.41151431307864	34.87921677130999	18.064427856914822
19	20.83888230107331	29.06838152573809	30.609018212776306	19.483717960412296
20	19.012262346720664	30.74738592794617	32.57199149875491	17.66836022657825
21	21.985229059044237	27.913977056088836	29.197996892568618	20.902796992298313
22	18.74205651806401	30.798209714004244	30.803378678326226	19.65635508960552
23	20.77506651463322	29.566552641581147	30.509768148992777	19.148612694792856
24	19.943988493618882	31.73985361596541	30.13066310690535	18.185494783510357
25	20.806814069679398	29.685661200125285	31.919817302166038	17.58770742802928
26	17.14404746337918	29.82699951040466	34.691022767703735	18.337930258512422
27	18.343853759988324	31.096982522713173	32.486530205178724	18.072633512119772
28	17.650778979117764	30.721635065614244	32.27107986816341	19.35650608710458
29	18.255541703408642	29.325569373916743	31.86456654605163	20.554322376622984
30	19.714687049637885	29.874552298252976	31.827413636888803	18.58334701522034
31	18.836349484335475	28.747081144191476	32.60331533372251	19.813254037750536
32	16.89288211864149	31.638845814710088	32.617896561160265	18.850375505488156
33	18.89802086468966	30.389411367637535	31.80907379872963	18.903493968943174
34	17.685157311877983	31.08988685464425	34.1096302783175	17.11532555516026
35	19.28108351375678	31.807234777531807	31.125173328143624	17.786508380567785
36	18.295682952268297	30.718332172754888	31.826101763977878	19.159883110998937
37	19.41686760176235	30.759337020606363	30.007084672478328	19.81671070515296
38	18.594193248260073	32.41084486443919	30.68475157574044	18.310210311560297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	88.0
2	112.0
3	112.0
4	391.5
5	671.0
6	671.0
7	867.5
8	1064.0
9	1146.0
10	1228.0
11	1228.0
12	1541.0
13	1854.0
14	2455.5
15	3057.0
16	3057.0
17	4133.0
18	5209.0
19	5209.0
20	5836.0
21	6463.0
22	7900.5
23	9338.0
24	9338.0
25	11757.0
26	14176.0
27	14176.0
28	17609.0
29	21042.0
30	23694.0
31	26346.0
32	26346.0
33	28172.5
34	29999.0
35	29999.0
36	30183.0
37	30367.0
38	31823.0
39	33279.0
40	33279.0
41	31563.5
42	29848.0
43	28498.5
44	27149.0
45	27149.0
46	26330.5
47	25512.0
48	25512.0
49	23073.0
50	20634.0
51	15888.5
52	11143.0
53	11143.0
54	10003.5
55	8864.0
56	8864.0
57	7399.0
58	5934.0
59	5152.0
60	4370.0
61	4370.0
62	3958.5
63	3547.0
64	2983.0
65	2419.0
66	2419.0
67	2128.0
68	1837.0
69	1837.0
70	1561.0
71	1285.0
72	1089.5
73	894.0
74	894.0
75	692.0
76	490.0
77	490.0
78	383.5
79	277.0
80	207.5
81	138.0
82	138.0
83	106.0
84	74.0
85	74.0
86	52.5
87	31.0
88	22.5
89	14.0
90	14.0
91	13.5
92	13.0
93	11.0
94	9.0
95	9.0
96	9.5
97	10.0
98	10.0
99	84.0
100	158.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12252256634612169
2	0.05229250970603705
3	0.010032865234297807
4	0.002432209753769165
5	0.0
6	6.080524384422912E-4
7	0.0
8	3.040262192211456E-4
9	0.0
10	0.0
11	0.003952340849874893
12	0.0033442884114326015
13	0.0173294944956053
14	0.011552996330403534
15	0.02705833351068196
16	0.013681179864951553
17	0.022497940222364777
18	0.007296629261307495
19	0.008816760357413222
20	0.007904681699749786
21	0.00912078657663437
22	0.010032865234297807
23	0.013377153645730406
24	0.017633520714826444
25	0.021281835345480195
26	0.023105992660807068
27	0.010640917672740098
28	0.008208707918970931
29	0.014897284741836134
30	0.005168445726759475
31	0.006992603042086349
32	0.008816760357413222
33	0.011552996330403534
34	0.015201310961057281
35	0.019153651810932173
36	0.01824157315326874
37	0.012161048768845826
38	0.007904681699749786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	328919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.83597789167602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79016130746926	22.137198269169776
2	13.363769949608143	8.241698294311222
3	5.123621676439083	4.739756542199629
4	2.417889029433214	2.9823189062451427
5	1.4922976373296222	2.3008228476248296
6	0.961546808886239	1.7790141684376617
7	0.681678688588332	1.4714160279375559
8	0.518755481786346	1.2797066054039608
9	0.357831334209823	0.9930671199578732
>10	2.5507976673072115	15.711850581525722
>50	0.3818200270028578	8.28295648092098
>100	0.32684593934537826	20.849169872438033
>500	0.022989163929491435	4.65064859494741
>1k	0.009995288664996277	4.580375688880207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3059	0.9300162045974845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2323	0.7062529072507212	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1606	0.48826610806915993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1208	0.36726367281914396	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	1177	0.3578388600232884	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1168	0.3551026240502981	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	1161	0.3529744405157501	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1086	0.33017247407416417	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	1039	0.3158832417707703	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	1034	0.3143631106746646	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	951	0.28912893447930954	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	940	0.2857846460678769	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	895	0.2721034662029253	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	737	0.22406732356598436	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	733	0.22285121868909977	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	724	0.22011498271610944	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	679	0.20643380285115787	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	679	0.20643380285115787	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	678	0.20612977663193674	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	665	0.20217743578206182	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	652	0.19822509493218696	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	632	0.19214457054776404	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT	606	0.18423988884801426	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	603	0.18332781019035083	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT	594	0.1805915742173605	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	591	0.17967949555969706	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	590	0.17937546934047593	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	534	0.16235000106409178	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	532	0.16174194862564947	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	528	0.1605258437487649	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	519	0.15778960777577458	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC	516	0.15687752911811115	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	511	0.1553573980220054	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT	494	0.15018895229524595	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	487	0.14806076876069793	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC	481	0.14623661144537106	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	477	0.14502050656848647	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA	475	0.14441245413004417	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA	471	0.14319634925315958	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA	468	0.14228427059549614	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	467	0.141980244376275	No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAA	459	0.13954803462250587	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	459	0.13954803462250587	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG	444	0.13498764133418867	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG	436	0.1325554315804195	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	431	0.13103530048431375	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT	429	0.13042724804587147	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	428	0.13012322182665031	No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG	417	0.12677893341521773	No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG	413	0.12556282853833314	No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA	411	0.12495477609989086	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	408	0.12404269744222741	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG	407	0.12373867122300627	No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA	401	0.12191451390767939	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG	401	0.12191451390767939	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA	399	0.1213064614692371	No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT	399	0.1213064614692371	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.1213064614692371	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	394	0.11978633037313137	No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC	393	0.11948230415391023	No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATAT	383	0.11644204196169879	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	381	0.11583398952325649	No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGG	380	0.11552996330403535	No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC	378	0.11492191086559306	No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA	377	0.1146178846463719	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	374	0.11370580598870847	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	365	0.11096957001571815	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	365	0.11096957001571815	No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT	363	0.11036151757727586	No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA	354	0.10762528160428554	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	353	0.1073212553850644	No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA	353	0.1073212553850644	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT	350	0.10640917672740098	No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGT	347	0.10549709806973755	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	347	0.10549709806973755	No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTAT	346	0.10519307185051639	No Hit
GTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAG	340	0.10336891453518952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.040262192211456E-4	0.0
9	0.0	0.0	0.0	3.040262192211456E-4	0.0
10	0.0	0.0	0.0	3.040262192211456E-4	0.0
11	0.0	0.0	0.0	3.040262192211456E-4	0.0
12	0.0	0.0	0.0	3.040262192211456E-4	0.0
13	0.0	0.0	0.0	3.040262192211456E-4	0.0
14	0.0	0.0	0.0	3.040262192211456E-4	0.0
15	0.0	0.0	0.0	6.080524384422912E-4	0.0
16	0.0	0.0	0.0	9.120786576634369E-4	0.0
17	0.0	0.0	0.0	9.120786576634369E-4	0.0
18	0.0	0.0	0.0	0.0018241573153268738	0.0
19	0.0	0.0	0.0	0.002432209753769165	0.0
20	0.0	0.0	0.0	0.0027362359729903108	0.0
21	0.0	0.0	0.0	0.003952340849874893	0.0
22	0.0	0.0	0.0	0.0063845506036440585	0.0
23	0.0	0.0	0.0	0.007904681699749786	0.0
24	0.0	0.0	0.0	0.012161048768845825	0.0
25	0.0	0.0	0.0	0.014897284741836136	0.0
26	0.0	0.0	0.0	0.016417415837941863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAC	25	2.8867213E-4	32.0073	1
CAAGTTT	30	8.4002933E-4	26.668695	28
TAAATTC	40	1.58936E-4	24.001823	16
TTGAACT	35	0.002062511	22.858881	9
TCAAGTT	35	0.002062511	22.858881	27
CGTATGT	115	0.0	22.262562	23
GACGTGG	110	1.8189894E-12	21.816523	7
ATAGGAC	45	3.538434E-4	21.325226	3
CTACACT	45	3.538434E-4	21.325226	4
CTCGGAG	115	1.8189894E-12	20.871153	31
TAGGACC	170	0.0	20.698011	4
CTGTAGG	350	0.0	20.576122	1
TGTAGGA	350	0.0	20.566736	2
TCAGGAC	95	1.718945E-9	20.202845	3
TCGGAGA	120	3.6379788E-12	20.001522	32
TGAACTT	40	0.0044748224	20.00152	10
ACGATTA	80	1.2576311E-7	20.00152	32
TCGTCCC	40	0.0044748224	20.00152	13
GCCGTTT	40	0.0044748224	20.00152	11
CTGTGCA	40	0.0044748224	20.00152	9
>>END_MODULE