FastQCFastQC Report
Thu 2 Feb 2017
SRR4063060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328919
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29550.8983974777984854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22570.6861871767821257No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15960.4852258458769484No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15440.4694164824774489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13770.4186441038675175No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT13190.40101058315269106No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA11390.3462858636928849No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11040.3356449460201448No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA10100.3070664814133571No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT9790.2976416686175016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.2952094588637324No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC9160.2784880168065694No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT8800.26754307291460816No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG8650.26298267962629096No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT8390.2550779979265412No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA8150.24778136866523368No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG6900.2097780912625905No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6900.2097780912625905No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA6880.2091700388241482No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT6850.20825796016648473No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC6700.2036975668781676No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC6500.19761704249374465No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT5980.18180767909424508No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC5930.18028754799813937No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT5920.17998352177891822No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG5830.1772472858059279No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA5800.17633520714826445No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT5670.1723828662983896No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC5660.17207884007916843No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA5430.16508623703708208No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT5380.16356610594097634No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT5230.15900571265265917No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG5140.15626947667966884No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT5110.1553573980220054No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG5100.15505337180278428No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC5060.1538372669258997No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5050.15353324070667854No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA4990.15170908339135167No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT4940.15018895229524595No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA4930.1498849260760248No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA4850.14745271632225562No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAA4790.14562855900692875No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4770.14502050656848647No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA4690.1425882968147173No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4680.14228427059549614No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG4650.1413721919378327No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA4470.1358997199918521No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC4420.13437958889574636No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA4390.13346751023808293No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4380.13316348401886177No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA4380.13316348401886177No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT4350.13225140536119834No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC4280.13012322182665031No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGG4190.12738698585366004No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA4150.12617088097677545No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC4110.12495477609989086No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG4060.12343464500378512No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC4060.12343464500378512No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG4030.12252256634612169No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG3990.1213064614692371No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC3990.1213064614692371No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG3960.12039438281157366No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG3960.12039438281157366No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC3900.11857022549624678No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG3880.1179621730578045No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT3870.11765814683858337No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATAT3840.11674606818091993No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA3770.1146178846463719No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA3720.11309775355026618No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT3680.11188164867338159No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA3660.11127359623493929No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3630.11036151757727586No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3550.1079293078235067No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.10762528160428554No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTA3510.10671320294662211No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT3440.1045850194120741No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT3410.10367294075441066No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTT3400.10336891453518952No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT3390.10306488831596837No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA3350.1018487834390838No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3350.1018487834390838No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTAT3310.10063267856219921No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.10032865234297805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACC200.003746512832.0054744
GTCTTGC252.8902676E-432.000611
TTAACCG200.003752096831.99574332
AGAGTGT356.39618E-527.441616
ATAGGCC308.4105646E-426.6631215
TGTCCAC557.551898E-826.18629810
AAACTTG552.201412E-623.2837896
ATACTGT350.002057729822.8680086
TACACAG350.002057729822.8680085
CCGTATT350.002065021522.85410327
AAATGTC653.7820246E-722.1643777
ATGTCCA653.7875907E-722.1610059
TAGAAAT805.2077667E-922.0037634
AACTTGC604.6843215E-621.3434737
ATTGAAC453.521659E-421.3402278
GGTAACC453.5325324E-421.33049631
ACTGTTC4250.020.7125768
ACAGGCG851.051194E-820.70312725
TGAGCTT555.8094312E-520.3671210
ATACACT4800.020.3368114
Produced by FastQC (version 0.11.5)