##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328919
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.930335432127666	32.0	32.0	32.0	32.0	32.0
2	31.281351943791634	32.0	32.0	32.0	32.0	32.0
3	31.377865067083384	32.0	32.0	32.0	32.0	32.0
4	31.481890678252093	32.0	32.0	32.0	32.0	32.0
5	31.40817040061535	32.0	32.0	32.0	32.0	32.0
6	34.85886494851316	36.0	36.0	36.0	32.0	36.0
7	34.91004776251904	36.0	36.0	36.0	36.0	36.0
8	34.84917867316877	36.0	36.0	36.0	32.0	36.0
9	34.99212876118436	36.0	36.0	36.0	36.0	36.0
10	34.85438056177965	36.0	36.0	36.0	32.0	36.0
11	35.01228873978092	36.0	36.0	36.0	36.0	36.0
12	34.91864866426081	36.0	36.0	36.0	32.0	36.0
13	34.98162465531028	36.0	36.0	36.0	36.0	36.0
14	34.92839878511123	36.0	36.0	36.0	32.0	36.0
15	34.90888942262381	36.0	36.0	36.0	32.0	36.0
16	34.892915885065925	36.0	36.0	36.0	32.0	36.0
17	34.89249024835902	36.0	36.0	36.0	32.0	36.0
18	34.87951744958485	36.0	36.0	36.0	32.0	36.0
19	34.84396158324694	36.0	36.0	36.0	32.0	36.0
20	34.79203390500397	36.0	36.0	36.0	32.0	36.0
21	34.7699676820129	36.0	36.0	36.0	32.0	36.0
22	34.73379768271216	36.0	36.0	36.0	32.0	36.0
23	34.70770919284079	36.0	36.0	36.0	32.0	36.0
24	34.686071646818824	36.0	36.0	36.0	32.0	36.0
25	34.66911610457286	36.0	36.0	36.0	32.0	36.0
26	34.61874200031011	36.0	36.0	36.0	32.0	36.0
27	34.57438761518793	36.0	36.0	36.0	32.0	36.0
28	34.538989842484014	36.0	36.0	36.0	32.0	36.0
29	34.478105551822786	36.0	36.0	36.0	32.0	36.0
30	34.43056801218537	36.0	36.0	36.0	32.0	36.0
31	34.41696283887522	36.0	36.0	36.0	32.0	36.0
32	34.35347304351527	36.0	36.0	36.0	32.0	36.0
33	34.305227122787066	36.0	36.0	36.0	32.0	36.0
34	34.26349040341239	36.0	36.0	36.0	32.0	36.0
35	34.1749154047045	36.0	36.0	36.0	32.0	36.0
36	34.11145297170428	36.0	36.0	36.0	32.0	36.0
37	34.067998504191	36.0	36.0	36.0	32.0	36.0
38	33.67536080311566	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	7.0
22	21.0
23	64.0
24	211.0
25	492.0
26	1042.0
27	2181.0
28	4028.0
29	6751.0
30	10095.0
31	14730.0
32	20756.0
33	32140.0
34	76238.0
35	160160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35300019763146	23.029842350902264	13.627449489958801	22.98970796150747
2	12.01146786898902	23.72924641021042	42.93184644243719	21.327439278363368
3	17.303956293190726	29.53888343330729	32.31525086723479	20.8419094062672
4	8.859628054323405	19.84591951209872	43.236784740316004	28.057667693261866
5	8.957220470693393	42.522019098927096	37.54632599515382	10.974434435225694
6	25.60326402548956	43.71957837643918	19.593577750145172	11.083579847926085
7	23.264531781574075	36.04313550226957	23.23808133966928	17.454251376487075
8	23.84473422578419	38.425147534105385	21.40757113712995	16.32254710298048
9	25.46509824905977	15.768495237284478	22.501360558695566	36.265045954960186
10	13.704410852618906	29.899427209378686	36.39409454085541	20.00206739714699
11	31.642636926949997	25.182275355885412	26.483590657285845	16.691497059878746
12	20.880584218559033	29.327066320481094	33.60047185012678	16.191877610833096
13	26.409237532644813	23.659320379789552	29.39325487430036	20.53818721326527
14	20.088532435037198	25.5448909913991	29.556516954022115	24.810059619541587
15	22.527126739410004	31.600181199626654	27.480625929180132	18.392066131783206
16	20.469477287721293	30.357626041669832	29.872400195792885	19.300496474815986
17	17.354120619362217	29.389910585888927	31.508061255202648	21.747907539546212
18	17.80955189575549	29.556820980241337	34.346146011632044	18.287481112371132
19	20.806946391056766	29.33853015484055	30.503558626895984	19.3509648272067
20	19.22327381513382	30.975711345346426	32.003319966313896	17.797694873205867
21	22.073215594112835	28.042466382300805	28.896172005873787	20.988146017712566
22	18.803413606389416	30.6993515120744	30.491093551907916	20.006141329628267
23	20.775631690476988	29.39903137246556	30.15696873698388	19.668368200073573
24	19.880274474870713	32.06716547235033	29.690288490479418	18.362271562299533
25	20.89541802085012	29.81037884707177	31.287642246267318	18.006560885810792
26	17.27355367126861	29.864495514093136	34.3397614610284	18.522189353609857
27	18.67785077785108	31.225316871326985	32.11459356254883	17.9822387882731
28	17.647505920910618	30.706040088897268	32.17509478017384	19.47135921001827
29	18.24704562521472	29.186821071449202	31.83063307379628	20.735500229539795
30	19.58020059649944	30.2104773515668	31.46367342719636	18.745648624737395
31	19.070956679304025	28.912589421711726	31.865596089006715	20.15085780997753
32	16.750020521769798	31.42050170406696	32.413147309824	19.416330464339246
33	18.912863045309027	30.651923421875903	31.424454044916832	19.010759487898234
34	17.7104393482894	31.053542057467038	33.887066420608114	17.348952173635453
35	19.59783411721427	32.01335283154819	30.441233251955644	17.94757979928189
36	18.25555835935291	30.875382693003445	31.49164383936471	19.37741510827894
37	19.504802094132597	30.71303269193935	29.728291767882066	20.053873446045987
38	18.731359392434	32.2003289563692	30.37829982457687	18.69001182661993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	64.0
2	72.0
3	72.0
4	276.0
5	480.0
6	480.0
7	654.0
8	828.0
9	1002.5
10	1177.0
11	1177.0
12	1440.5
13	1704.0
14	2330.0
15	2956.0
16	2956.0
17	3989.5
18	5023.0
19	5023.0
20	5660.0
21	6297.0
22	7888.5
23	9480.0
24	9480.0
25	11943.5
26	14407.0
27	14407.0
28	18005.0
29	21603.0
30	24303.0
31	27003.0
32	27003.0
33	28512.0
34	30021.0
35	30021.0
36	30055.5
37	30090.0
38	31432.0
39	32774.0
40	32774.0
41	30903.5
42	29033.0
43	27750.0
44	26467.0
45	26467.0
46	26344.5
47	26222.0
48	26222.0
49	23921.0
50	21620.0
51	16713.0
52	11806.0
53	11806.0
54	10249.5
55	8693.0
56	8693.0
57	7239.5
58	5786.0
59	5081.5
60	4377.0
61	4377.0
62	3934.5
63	3492.0
64	3007.5
65	2523.0
66	2523.0
67	2154.5
68	1786.0
69	1786.0
70	1580.5
71	1375.0
72	1103.5
73	832.0
74	832.0
75	630.5
76	429.0
77	429.0
78	352.0
79	275.0
80	205.5
81	136.0
82	136.0
83	93.0
84	50.0
85	50.0
86	36.5
87	23.0
88	14.5
89	6.0
90	6.0
91	5.5
92	5.0
93	5.5
94	6.0
95	6.0
96	5.0
97	4.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007296629261307495
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	6.080524384422912E-4
8	0.0036483146306537477
9	0.0030402621922114566
10	9.120786576634369E-4
11	0.0063845506036440585
12	3.040262192211456E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	328919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.004101313697294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94758909853249	20.28766960862705
2	14.267295597484278	8.276201739638028
3	5.3417190775681345	4.647952839452874
4	2.653039832285115	3.0779614433948783
5	1.5838574423480085	2.296918086215755
6	1.0136268343815513	1.7639601239210871
7	0.6949685534591195	1.410985683405337
8	0.5104821802935011	1.1844861500855834
9	0.42033542976939203	1.0972306251691146
>10	2.752620545073375	15.934926228037908
>50	0.42662473794549266	8.764467847707186
>100	0.34905660377358494	21.264505850984587
>500	0.029350104821802933	5.644854812279012
>1k	0.009433962264150943	4.347878961081603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2955	0.8983974777984854	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2257	0.6861871767821257	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1596	0.4852258458769484	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1544	0.4694164824774489	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1377	0.4186441038675175	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	1319	0.40101058315269106	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	1139	0.3462858636928849	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1104	0.3356449460201448	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	1010	0.3070664814133571	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	979	0.2976416686175016	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.2952094588637324	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	916	0.2784880168065694	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	880	0.26754307291460816	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	865	0.26298267962629096	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	839	0.2550779979265412	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	815	0.24778136866523368	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	690	0.2097780912625905	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	690	0.2097780912625905	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	688	0.2091700388241482	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	685	0.20825796016648473	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	670	0.2036975668781676	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC	650	0.19761704249374465	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT	598	0.18180767909424508	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	593	0.18028754799813937	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT	592	0.17998352177891822	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	583	0.1772472858059279	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	580	0.17633520714826445	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	567	0.1723828662983896	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	566	0.17207884007916843	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	543	0.16508623703708208	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	538	0.16356610594097634	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT	523	0.15900571265265917	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	514	0.15626947667966884	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	511	0.1553573980220054	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	510	0.15505337180278428	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	506	0.1538372669258997	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	505	0.15353324070667854	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	499	0.15170908339135167	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	494	0.15018895229524595	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA	493	0.1498849260760248	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA	485	0.14745271632225562	No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAA	479	0.14562855900692875	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	477	0.14502050656848647	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA	469	0.1425882968147173	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	468	0.14228427059549614	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	465	0.1413721919378327	No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA	447	0.1358997199918521	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	442	0.13437958889574636	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	439	0.13346751023808293	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG	438	0.13316348401886177	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA	438	0.13316348401886177	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT	435	0.13225140536119834	No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC	428	0.13012322182665031	No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGG	419	0.12738698585366004	No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA	415	0.12617088097677545	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	411	0.12495477609989086	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG	406	0.12343464500378512	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	406	0.12343464500378512	No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG	403	0.12252256634612169	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG	399	0.1213064614692371	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC	399	0.1213064614692371	No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCG	396	0.12039438281157366	No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATG	396	0.12039438281157366	No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC	390	0.11857022549624678	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG	388	0.1179621730578045	No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT	387	0.11765814683858337	No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATAT	384	0.11674606818091993	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	377	0.1146178846463719	No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA	372	0.11309775355026618	No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTT	368	0.11188164867338159	No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA	366	0.11127359623493929	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	363	0.11036151757727586	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	355	0.1079293078235067	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	354	0.10762528160428554	No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTA	351	0.10671320294662211	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT	344	0.1045850194120741	No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATT	341	0.10367294075441066	No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTT	340	0.10336891453518952	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	339	0.10306488831596837	No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA	335	0.1018487834390838	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	335	0.1018487834390838	No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTAT	331	0.10063267856219921	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	330	0.10032865234297805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.040262192211456E-4	0.0
16	0.0	0.0	0.0	6.080524384422912E-4	0.0
17	0.0	0.0	0.0	6.080524384422912E-4	0.0
18	0.0	0.0	0.0	0.0012161048768845825	0.0
19	0.0	0.0	0.0	0.001520131096105728	0.0
20	0.0	0.0	0.0	0.0027362359729903108	0.0
21	0.0	0.0	0.0	0.003952340849874893	0.0
22	0.0	0.0	0.0	0.0063845506036440585	0.0
23	0.0	0.0	0.0	0.007904681699749786	0.0
24	0.0	0.0	0.0	0.01185702254962468	0.0
25	0.0	0.0	0.0	0.014289232303393845	0.0
26	0.0	0.0	0.0	0.015505337180278427	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACC	20	0.0037465128	32.005474	4
GTCTTGC	25	2.8902676E-4	32.00061	1
TTAACCG	20	0.0037520968	31.995743	32
AGAGTGT	35	6.39618E-5	27.44161	6
ATAGGCC	30	8.4105646E-4	26.66312	15
TGTCCAC	55	7.551898E-8	26.186298	10
AAACTTG	55	2.201412E-6	23.283789	6
ATACTGT	35	0.0020577298	22.868008	6
TACACAG	35	0.0020577298	22.868008	5
CCGTATT	35	0.0020650215	22.854103	27
AAATGTC	65	3.7820246E-7	22.164377	7
ATGTCCA	65	3.7875907E-7	22.161005	9
TAGAAAT	80	5.2077667E-9	22.003763	4
AACTTGC	60	4.6843215E-6	21.343473	7
ATTGAAC	45	3.521659E-4	21.340227	8
GGTAACC	45	3.5325324E-4	21.330496	31
ACTGTTC	425	0.0	20.712576	8
ACAGGCG	85	1.051194E-8	20.703127	25
TGAGCTT	55	5.8094312E-5	20.36712	10
ATACACT	480	0.0	20.336811	4
>>END_MODULE