FastQCFastQC Report
Thu 2 Feb 2017
SRR4063059_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063059_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197861
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39230.3275004361941828No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30340.253284813513421No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28770.24017811749443385No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28530.23817454612847402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26800.2237321358655136No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26290.21947454671284897No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25230.21062543984652643No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25200.21037499342578145No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25130.20979061844404318No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25070.2092897256025532No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24090.20110847585821726No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT23330.19476383319934448No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG23090.19276026183338468No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG22980.1918419582906531No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22060.1841616013878071No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21990.1835772264060688No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA21650.18073883363762575No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21500.17948660153390084No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT20010.16704776263690027No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT19670.1642093698684572No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC19450.162372762782994No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT19130.1597013342950476No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18930.15803169149008106No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT18270.15252187023369154No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18020.1504348167274834No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA17990.15018437030673842No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG17690.14767990609928866No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT17640.14726249539804703No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT17190.14350579908687233No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG16740.1397491027756977No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG16360.1365767814462613No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15630.1304825852081335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15620.13039910306788519No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA15310.12781115672018706No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG15270.12747722815919377No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15160.12655892461646218No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC15150.12647544247621384No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA14550.12146651406131428No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC14050.117292407048898No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14010.1169584784879047No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC13860.1157062463841798No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA13570.11328526431697834No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT13530.11295133575598504No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC13240.11053035368878358No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG13080.10919463944481038No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT13020.10869374660332043No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC13000.10852678232282377No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12600.10518749671289072No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT12580.10502053243239408No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA12570.10493705029214576No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC12570.10493705029214576No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG12310.10276651464568927No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG12220.10201517538345434No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC12180.10168124682246103No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG12070.10076294327972944No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT11980.10001160401749451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG200.003753746931.99970630
TAGGCGT356.431272E-527.42832231
ATCCGCC552.2188378E-623.27251631
TGTCGAG707.791823E-720.56952318
GTCGAGG400.004484908719.99898119
GTCGAGA400.004484908719.99898119
AATCCGC659.4351E-619.6921330
ACCTACT659.4419065E-619.6904838
TGCTATA450.00883180517.7842982
AGGACCT7150.017.4500165
GTTATAT1404.0017767E-1117.1627661
AACTATC753.2352873E-517.06579819
GTATTAG5300.016.9252951
TAGAAAT5400.016.88384
AAATGTC5650.016.7064177
GTCCTAA8700.016.5709461
TGTCCAC6100.016.5251310
CCCGTTG1551.0913936E-1116.51597831
AAGTGTG8150.016.4892717
TAACCCG1651.8189894E-1216.4840128
Produced by FastQC (version 0.11.5)