##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063059_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197861
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256654152693844	32.0	32.0	32.0	32.0	32.0
2	30.912652636658176	32.0	32.0	32.0	32.0	32.0
3	30.96597184481338	32.0	32.0	32.0	32.0	32.0
4	31.023694735866684	32.0	32.0	32.0	32.0	32.0
5	30.971956679447782	32.0	32.0	32.0	32.0	32.0
6	34.62023807436756	36.0	36.0	36.0	32.0	36.0
7	34.51731043919119	36.0	36.0	36.0	32.0	36.0
8	34.51729040347753	36.0	36.0	36.0	32.0	36.0
9	34.61929305653995	36.0	36.0	36.0	32.0	36.0
10	34.38336584962696	36.0	36.0	36.0	32.0	36.0
11	34.61139314160825	36.0	36.0	36.0	32.0	36.0
12	34.42813899108494	36.0	36.0	36.0	32.0	36.0
13	34.531545813746334	36.0	36.0	36.0	32.0	36.0
14	34.42835520982818	36.0	36.0	36.0	32.0	36.0
15	34.37779007747977	36.0	36.0	36.0	32.0	36.0
16	34.39260732255245	36.0	36.0	36.0	32.0	36.0
17	34.302520075367674	36.0	36.0	36.0	32.0	36.0
18	34.343926382109444	36.0	36.0	36.0	32.0	36.0
19	34.31714197223217	36.0	36.0	36.0	32.0	36.0
20	34.28258120099077	36.0	36.0	36.0	32.0	36.0
21	34.2568394830452	36.0	36.0	36.0	32.0	36.0
22	34.22743540360693	36.0	36.0	36.0	32.0	36.0
23	34.19073915921797	36.0	36.0	36.0	32.0	36.0
24	34.15974391018658	36.0	36.0	36.0	32.0	36.0
25	34.14193967413581	36.0	36.0	36.0	32.0	36.0
26	34.102431751263296	36.0	36.0	36.0	32.0	36.0
27	34.08373926524029	36.0	36.0	36.0	32.0	36.0
28	34.06161148914607	36.0	36.0	36.0	32.0	36.0
29	34.018934584229726	36.0	36.0	36.0	32.0	36.0
30	33.975057206136604	36.0	36.0	36.0	32.0	36.0
31	33.99721920990833	36.0	36.0	36.0	32.0	36.0
32	33.94105409559206	36.0	36.0	36.0	32.0	36.0
33	33.90004098973086	36.0	36.0	36.0	32.0	36.0
34	33.91000792245511	36.0	36.0	36.0	32.0	36.0
35	33.859171473150894	36.0	36.0	36.0	32.0	36.0
36	33.79144825651724	36.0	36.0	36.0	32.0	36.0
37	33.79785133667429	36.0	36.0	36.0	32.0	36.0
38	33.14096627238052	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	2.0
10	9.0
11	10.0
12	6.0
13	12.0
14	186.0
15	368.0
16	488.0
17	550.0
18	716.0
19	967.0
20	1209.0
21	1732.0
22	2518.0
23	3618.0
24	5345.0
25	7782.0
26	11289.0
27	16092.0
28	22550.0
29	30254.0
30	41145.0
31	55047.0
32	75349.0
33	114763.0
34	263461.0
35	542388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17383134761756	18.71966886719749	11.646235554314774	25.460264230870177
2	15.775252105508025	20.72473077144745	39.617128724366594	23.882888398677935
3	17.869016706882302	25.86711307411644	29.96326322732548	26.300606991675778
4	11.27080743578591	16.06013880111435	38.484458132170786	34.18459563092895
5	13.270499056651028	38.02698145025295	34.14998413838011	14.552535354715909
6	31.649608468435375	38.319252667256606	17.56991635082564	12.461222513482378
7	28.480516520698146	31.861376236474847	22.06558189973628	17.592525343090728
8	26.72514331844285	34.35635472347701	20.37947768389716	18.539024274182978
9	27.869534002304107	13.803282520494884	19.903745011937957	38.423438465263054
10	16.281896518619067	25.873350597483174	31.59456296155304	26.25018992234472
11	37.253399252152	20.797320503540966	24.259728160008414	17.689552084298615
12	25.50054974274377	24.43929996919437	29.3727923675504	20.687357920511456
13	29.004029369477983	19.539686701076892	26.35028963702278	25.105994292422345
14	22.94570355614406	22.43575079440087	24.979670350772025	29.638875298683036
15	24.061971073596915	29.96056249587714	22.586175079806043	23.391291350719904
16	24.478033882399302	25.736246285685183	26.225337742781978	23.560382089133537
17	22.383736464867106	26.863773605071373	26.72892326807078	24.023566661990742
18	24.047894753100078	23.93393516241955	29.74311922009897	22.275050864381406
19	25.770222997737463	24.62693171476995	26.004992611269273	23.597852676223315
20	25.347700820756774	24.687822727891287	25.929685915059064	24.034790536292878
21	27.50346262411389	24.074066343794534	24.153712959940425	24.26875807215115
22	25.525139925996182	25.723841519616357	25.229842641695942	23.521175912691522
23	22.515595882954067	24.951742931758826	27.04391226230472	25.488748922982385
24	24.798905901153713	25.864115995852043	26.13546987637952	23.201508226614724
25	23.956158097367634	25.219697962789322	26.809622374165777	24.01452156567727
26	22.563485112007502	27.096805976405324	28.041828825451326	22.29788008613585
27	24.191179724202534	25.873148527713745	25.828815884277606	24.10685586380611
28	22.185487964521386	26.068549464347733	27.05603310090567	24.689929470225213
29	23.253942212882418	25.18591248024406	26.62695858286558	24.933186724007943
30	22.446090012773098	25.9477555245736	27.775978227294356	23.83017623535894
31	24.801217573668886	25.035231492613075	25.337036814405174	24.82651411931287
32	23.137676597833657	26.21796359592512	25.392518480052228	25.251841326188995
33	22.318364811589653	25.43660739481598	27.443106203423394	24.80192159017097
34	24.12681793779332	25.526313812027386	27.604851563432764	22.742016686746535
35	25.387772141858182	25.54549439539107	26.863214144070803	22.203519318679945
36	22.732684642320994	26.48831838516349	26.42160685450541	24.35739011801011
37	24.537986736764942	26.368506871651526	25.25583449593278	23.83767189565075
38	23.15830338246528	26.664913445122078	26.308508551129368	23.868274621283277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	438.0
1	363.0
2	288.0
3	288.0
4	754.5
5	1221.0
6	1221.0
7	1382.0
8	1543.0
9	1492.5
10	1442.0
11	1442.0
12	1862.5
13	2283.0
14	2924.5
15	3566.0
16	3566.0
17	5088.5
18	6611.0
19	6611.0
20	7817.5
21	9024.0
22	10763.5
23	12503.0
24	12503.0
25	15869.0
26	19235.0
27	19235.0
28	25245.5
29	31256.0
30	38814.0
31	46372.0
32	46372.0
33	59858.5
34	73345.0
35	73345.0
36	74972.5
37	76600.0
38	82616.0
39	88632.0
40	88632.0
41	83073.5
42	77515.0
43	83202.5
44	88890.0
45	88890.0
46	83049.0
47	77208.0
48	77208.0
49	84331.0
50	91454.0
51	91080.0
52	90706.0
53	90706.0
54	83344.0
55	75982.0
56	75982.0
57	76628.5
58	77275.0
59	67904.0
60	58533.0
61	58533.0
62	61893.5
63	65254.0
64	52725.5
65	40197.0
66	40197.0
67	34538.0
68	28879.0
69	28879.0
70	23427.0
71	17975.0
72	14081.5
73	10188.0
74	10188.0
75	7460.5
76	4733.0
77	4733.0
78	5413.0
79	6093.0
80	4926.0
81	3759.0
82	3759.0
83	4160.5
84	4562.0
85	4562.0
86	2963.5
87	1365.0
88	1244.5
89	1124.0
90	1124.0
91	805.0
92	486.0
93	428.0
94	370.0
95	370.0
96	330.0
97	290.0
98	290.0
99	477.0
100	664.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12380401398826742
2	0.05376249831992193
3	0.01260580317749722
4	0.0026714284879464315
5	8.348214024832599E-5
6	8.348214024832599E-5
7	0.0
8	3.3392856099330394E-4
9	8.348214024832599E-5
10	2.5044642074497796E-4
11	0.0020035713659598236
12	0.0023374999269531274
13	0.012939731738490525
14	0.008598660445577576
15	0.022540177867048015
16	0.010852678232282377
17	0.019785267238853255
18	0.005676785536886167
19	0.007596874762597663
20	0.005342856975892863
21	0.005843749817382818
22	0.007095981921107708
23	0.009099553287067531
24	0.013607588860477134
25	0.01619553520817524
26	0.018199106574135062
27	0.008181249744335945
28	0.005760267677134492
29	0.010017856829799117
30	0.0025879463476981054
31	0.005760267677134492
32	0.0069290176406110555
33	0.008598660445577576
34	0.012021428195758941
35	0.01552767808618863
36	0.013941517421470437
37	0.00951696398830916
38	0.0055098212563895145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1197861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.90295914169578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.338388409048	28.484829174180398
2	11.997686096460145	8.615048674322002
3	3.502325479498458	3.7723154577405973
4	1.507603493047437	2.165097064510399
5	0.7982974304942838	1.4330620014978503
6	0.4792617733680182	1.0324149520445172
7	0.3288190497111097	0.8263903834752444
8	0.24572770865926066	0.7057881507180769
9	0.17891506947751504	0.5781222386356396
>10	1.1320934273516718	8.09211256478677
>50	0.19458055581265987	4.999433665751721
>100	0.2391409801241763	18.86663006643231
>500	0.037096015447464985	9.011309108579116
>1k	0.020064511499886722	11.417446497325388
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3923	0.3275004361941828	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3034	0.253284813513421	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2877	0.24017811749443385	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2853	0.23817454612847402	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2680	0.2237321358655136	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2629	0.21947454671284897	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2523	0.21062543984652643	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2520	0.21037499342578145	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2513	0.20979061844404318	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2507	0.2092897256025532	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2409	0.20110847585821726	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2333	0.19476383319934448	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2309	0.19276026183338468	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2298	0.1918419582906531	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2206	0.1841616013878071	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2199	0.1835772264060688	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2165	0.18073883363762575	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2150	0.17948660153390084	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	2001	0.16704776263690027	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1967	0.1642093698684572	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1945	0.162372762782994	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1913	0.1597013342950476	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1893	0.15803169149008106	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1827	0.15252187023369154	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1802	0.1504348167274834	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	1799	0.15018437030673842	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1769	0.14767990609928866	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1764	0.14726249539804703	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1719	0.14350579908687233	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1674	0.1397491027756977	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1636	0.1365767814462613	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1563	0.1304825852081335	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1562	0.13039910306788519	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1531	0.12781115672018706	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	1527	0.12747722815919377	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1516	0.12655892461646218	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	1515	0.12647544247621384	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	1455	0.12146651406131428	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1405	0.117292407048898	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1401	0.1169584784879047	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	1386	0.1157062463841798	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA	1357	0.11328526431697834	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT	1353	0.11295133575598504	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1324	0.11053035368878358	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1308	0.10919463944481038	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	1302	0.10869374660332043	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1300	0.10852678232282377	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1260	0.10518749671289072	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1258	0.10502053243239408	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA	1257	0.10493705029214576	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1257	0.10493705029214576	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG	1231	0.10276651464568927	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	1222	0.10201517538345434	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC	1218	0.10168124682246103	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG	1207	0.10076294327972944	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	1198	0.10001160401749451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	8.348214024832597E-5	0.0
17	0.0	0.0	0.0	8.348214024832597E-5	0.0
18	0.0	0.0	0.0	8.348214024832597E-5	0.0
19	0.0	0.0	0.0	2.504464207449779E-4	0.0
20	0.0	0.0	0.0	3.339285609933039E-4	0.0
21	0.0	0.0	0.0	4.1741070124162987E-4	0.0
22	0.0	0.0	0.0	5.843749817382818E-4	0.0
23	0.0	0.0	0.0	0.0012522321037248896	0.0
24	0.0	0.0	0.0	0.0021705356464564755	0.0
25	0.0	0.0	0.0	0.0027549106281947572	0.0
26	0.0	0.0	0.0	0.003756696311174669	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	0.0037537469	31.999706	30
TAGGCGT	35	6.431272E-5	27.428322	31
ATCCGCC	55	2.2188378E-6	23.272516	31
TGTCGAG	70	7.791823E-7	20.569523	18
GTCGAGG	40	0.0044849087	19.998981	19
GTCGAGA	40	0.0044849087	19.998981	19
AATCCGC	65	9.4351E-6	19.69213	30
ACCTACT	65	9.4419065E-6	19.690483	8
TGCTATA	45	0.008831805	17.784298	2
AGGACCT	715	0.0	17.450016	5
GTTATAT	140	4.0017767E-11	17.162766	1
AACTATC	75	3.2352873E-5	17.065798	19
GTATTAG	530	0.0	16.925295	1
TAGAAAT	540	0.0	16.8838	4
AAATGTC	565	0.0	16.706417	7
GTCCTAA	870	0.0	16.570946	1
TGTCCAC	610	0.0	16.52513	10
CCCGTTG	155	1.0913936E-11	16.515978	31
AAGTGTG	815	0.0	16.489271	7
TAACCCG	165	1.8189894E-12	16.48401	28
>>END_MODULE