FastQCFastQC Report
Thu 2 Feb 2017
SRR4063059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197861
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44190.36890757775735245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33090.27624240208171064No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30410.25386918849515927No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29170.24351740310436684No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28070.23433436767705101No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27120.22640356435346004No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA26320.21972499313359395No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26280.21939106457260063No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26230.21897365387135906No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25980.2168866003651509No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG25960.21671963608465425No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24950.2082879399195733No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23250.19409597607735787No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22960.19167499401015645No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG22890.19109061902841817No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22410.18708347629649852No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT21630.1805718693571291No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT21620.18048838721688076No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC20660.17247410175304145No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20630.1722236553322965No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG20420.17047053038708165No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT20310.16955222684435006No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA20020.1671312447771486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19440.16228928064274567No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT18960.15828213791082604No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT18680.15594463798387292No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA17820.1487651739225169No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG17620.14709553111755036No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17430.14550937045283216No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG16870.14083437059892592No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG16760.13991606705619433No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC16670.13916472779395941No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT16630.1388307992329661No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT15830.1321522280131No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15630.1304825852081335No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA15500.12939731738490526No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA15470.12914687096416028No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT14990.12513972823224062No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA14720.12288571044553583No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT14560.12154999620156261No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG14210.11862812129287122No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT14210.11862812129287122No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC14120.11787678203063628No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13860.1157062463841798No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC13830.11545579996343483No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC13680.11420356785970993No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC13570.11328526431697834No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG13480.11253392505474341No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC13440.1121999964937501No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.11194955007300512No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA13390.11178258579250848No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC13310.11111472867052187No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC13070.10911115730456206No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT13060.10902767516431373No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC12930.1079424073410855No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCG12700.10602231811537399No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC12690.10593883597512567No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT12690.10593883597512567No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC12610.10527097885313906No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAG12470.10410222888966249No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG12460.10401874674941416No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12350.10310044320668257No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA12220.10201517538345434No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT12150.10143080040171605No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC12030.10042901471873614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT400.00448267420.0007041
TAGAAAT5250.018.8966924
TGTCCAC5100.018.82419210
AAATGTC5500.018.6203927
ATGTCCA5100.018.5112299
TTAGAAA5050.018.3768843
AATGTCC5600.018.2878848
GAAATGT5550.018.1643186
ATTTAGA5150.018.0200521
TTTAGAA5800.017.9309182
ACCGTCG5600.017.7163898
ATACCGT5700.017.686316
TACCGTC5700.017.686317
TATCCTT550.001363605817.4566175
TAAGGGT550.001363987517.4558894
TTGTGCC652.0985915E-417.2328157
CGGCATC4950.017.13048725
AGATACC5900.016.8148544
CCGTCGT5900.016.8148549
CGTCGTA5850.016.6843510
Produced by FastQC (version 0.11.5)