##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197861
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20996843540277	32.0	32.0	32.0	32.0	32.0
2	31.328731797762845	32.0	32.0	32.0	32.0	32.0
3	31.399917018752593	32.0	32.0	32.0	32.0	32.0
4	31.49120473911414	32.0	32.0	32.0	32.0	32.0
5	31.41743741552651	32.0	32.0	32.0	32.0	32.0
6	34.94272206875422	36.0	36.0	36.0	36.0	36.0
7	34.969489782203446	36.0	36.0	36.0	36.0	36.0
8	34.909731596570886	36.0	36.0	36.0	36.0	36.0
9	35.00838661580935	36.0	36.0	36.0	36.0	36.0
10	34.862095852523794	36.0	36.0	36.0	32.0	36.0
11	35.042263668322114	36.0	36.0	36.0	36.0	36.0
12	34.92200013190178	36.0	36.0	36.0	32.0	36.0
13	34.984852165651944	36.0	36.0	36.0	36.0	36.0
14	34.934029908311565	36.0	36.0	36.0	32.0	36.0
15	34.89727522642443	36.0	36.0	36.0	32.0	36.0
16	34.89892149423013	36.0	36.0	36.0	32.0	36.0
17	34.86924776747887	36.0	36.0	36.0	32.0	36.0
18	34.87112361117025	36.0	36.0	36.0	32.0	36.0
19	34.85203041087405	36.0	36.0	36.0	32.0	36.0
20	34.84290831740911	36.0	36.0	36.0	32.0	36.0
21	34.822025260025995	36.0	36.0	36.0	32.0	36.0
22	34.80419430969036	36.0	36.0	36.0	32.0	36.0
23	34.744573034767804	36.0	36.0	36.0	32.0	36.0
24	34.707101241295945	36.0	36.0	36.0	32.0	36.0
25	34.6866481169351	36.0	36.0	36.0	32.0	36.0
26	34.62286692696398	36.0	36.0	36.0	32.0	36.0
27	34.606850043535935	36.0	36.0	36.0	32.0	36.0
28	34.55006048281061	36.0	36.0	36.0	32.0	36.0
29	34.50516712707067	36.0	36.0	36.0	32.0	36.0
30	34.466275302393186	36.0	36.0	36.0	32.0	36.0
31	34.48169612333985	36.0	36.0	36.0	32.0	36.0
32	34.4378404506032	36.0	36.0	36.0	32.0	36.0
33	34.41034393806961	36.0	36.0	36.0	32.0	36.0
34	34.38263037197137	36.0	36.0	36.0	32.0	36.0
35	34.34541403384867	36.0	36.0	36.0	32.0	36.0
36	34.3033340262351	36.0	36.0	36.0	32.0	36.0
37	34.28200016529464	36.0	36.0	36.0	32.0	36.0
38	33.81474895668195	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	11.0
21	26.0
22	98.0
23	300.0
24	837.0
25	1962.0
26	4059.0
27	7991.0
28	14095.0
29	22240.0
30	33933.0
31	49055.0
32	70239.0
33	113457.0
34	278108.0
35	601448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60681933927215	19.994239294689297	12.340683102765974	26.05825826327258
2	14.73526561095152	21.785582801343395	39.031156369562076	24.447995218143006
3	16.992706165406503	26.584303187097667	30.354440122852317	26.06855052464351
4	10.745180146561612	16.840560400314562	38.826471919042156	33.58778753408167
5	12.655725497365722	38.493781832783604	34.01187616927173	14.838616500578949
6	31.617384335398125	38.71429048469771	17.250012522331488	12.41831265757267
7	28.04470001302331	32.003118916193316	22.310018266029527	17.64216280475384
8	26.525817872775253	34.6279489711838	20.221600172979038	18.62463298306191
9	27.67815623666839	13.614501787009804	19.7901839271805	38.91715804914131
10	16.07572945703235	26.22756070703757	31.49486201447101	26.201847821459072
11	37.064389606963424	20.78916214866898	24.405035222849854	17.741413021517747
12	25.332802385591613	24.49063244558203	29.360909222962988	20.815655945863366
13	29.001576980262282	19.48175870448859	26.359195865289653	25.15746844995947
14	22.831382632361045	22.36321439901157	25.072295593808956	29.733107374818424
15	23.97899255422791	30.10290843428411	22.452521619787273	23.465577391700705
16	24.473958163760237	25.861097406126422	25.96244472438789	23.702499705725455
17	22.343994837464447	27.104396920844735	26.45248488764556	24.09912335404525
18	24.09411442563035	23.83982782643395	29.557686576322297	22.5083711716134
19	25.75040008815714	24.49608093092604	26.12147820156095	23.63204077935587
20	25.26812376394256	24.760969761933982	25.92587954695912	24.045026927164336
21	27.521830597899587	24.02567912777787	24.232130632962114	24.220359641360425
22	25.440702586278864	25.728966657205348	25.34920608418346	23.481124672332328
23	22.372434796111058	24.779147444855735	27.191035999258677	25.657381759774534
24	24.65820324728829	25.87745990561509	26.249289358281136	23.21504748881548
25	23.919927136666335	25.24245341062679	26.81158633862583	24.026033114081038
26	22.627500185747763	26.97007415718518	28.0188602851249	22.383565371942154
27	24.39391915582789	25.58846609787454	25.83841183443808	24.17920291185948
28	22.27403680393635	25.836386692612916	27.264849594402023	24.624726909048714
29	23.35321043092646	24.832180027565805	26.792507644876995	25.022101896630744
30	22.4760635833373	25.630352770480048	27.973362518689566	23.92022112749309
31	24.708292531437287	24.71371887055343	25.5927858073683	24.985202790640983
32	23.186413114710305	25.94658311774071	25.682863036696247	25.184140730852743
33	22.405354210546964	25.281397424242048	27.54860538910608	24.764642976104906
34	24.080005943928388	25.195577784066764	27.81441252365675	22.9100037483481
35	25.447610365476464	25.343842065147793	26.837754964891587	22.370792604484162
36	22.817338572672455	26.290613017704057	26.457159887499465	24.43488852212402
37	24.64726708691576	26.159546057514188	25.30769429842027	23.88549255714979
38	23.2730870051592	26.17651478469938	26.55852937738968	23.991868832751738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	264.0
1	222.0
2	180.0
3	180.0
4	653.5
5	1127.0
6	1127.0
7	1270.5
8	1414.0
9	1481.5
10	1549.0
11	1549.0
12	1900.5
13	2252.0
14	2961.0
15	3670.0
16	3670.0
17	5360.0
18	7050.0
19	7050.0
20	8167.5
21	9285.0
22	10865.5
23	12446.0
24	12446.0
25	15642.5
26	18839.0
27	18839.0
28	24672.5
29	30506.0
30	38553.0
31	46600.0
32	46600.0
33	60301.0
34	74002.0
35	74002.0
36	75402.0
37	76802.0
38	82379.0
39	87956.0
40	87956.0
41	83053.5
42	78151.0
43	83906.0
44	89661.0
45	89661.0
46	83350.0
47	77039.0
48	77039.0
49	84295.5
50	91552.0
51	91092.5
52	90633.0
53	90633.0
54	83403.5
55	76174.0
56	76174.0
57	77263.5
58	78353.0
59	68753.0
60	59153.0
61	59153.0
62	62383.5
63	65614.0
64	53039.5
65	40465.0
66	40465.0
67	34740.5
68	29016.0
69	29016.0
70	23709.5
71	18403.0
72	14183.5
73	9964.0
74	9964.0
75	7206.5
76	4449.0
77	4449.0
78	5292.5
79	6136.0
80	4806.0
81	3476.0
82	3476.0
83	3639.0
84	3802.0
85	3802.0
86	2376.5
87	951.0
88	830.0
89	709.0
90	709.0
91	420.5
92	132.0
93	88.0
94	44.0
95	44.0
96	30.5
97	17.0
98	17.0
99	21.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007596874762597663
2	0.0
3	0.0
4	2.5044642074497796E-4
5	0.0
6	8.348214024832599E-5
7	7.513392622349338E-4
8	0.0023374999269531274
9	0.004174107012416299
10	0.0015026785244698676
11	0.004925446274651232
12	2.5044642074497796E-4
13	1.6696428049665197E-4
14	8.348214024832599E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	8.348214024832599E-5
22	8.348214024832599E-5
23	2.5044642074497796E-4
24	0.0
25	1.6696428049665197E-4
26	0.0
27	8.348214024832599E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.348214024832599E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1197861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.432894979263736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57811838786202	25.9366108475028
2	12.854966755654216	8.595575070074283
3	3.921461302308714	3.9331741165600205
4	1.601090432267441	2.1411635309720536
5	0.903419665208736	1.5101967394562636
6	0.5359844177525289	1.0751706449545273
7	0.3621694772343685	0.8475861867950043
8	0.25548750777197343	0.6833349612683374
9	0.18316855012638142	0.5511469409891384
>10	1.269644945298374	8.434762994587345
>50	0.20580494852541595	4.935752610516483
>100	0.2613386501467918	18.99440274309141
>500	0.04347267930171926	9.576929303635387
>1k	0.023872280541406533	12.784193309596969
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4419	0.36890757775735245	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3309	0.27624240208171064	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3041	0.25386918849515927	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2917	0.24351740310436684	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2807	0.23433436767705101	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2712	0.22640356435346004	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2632	0.21972499313359395	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2628	0.21939106457260063	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2623	0.21897365387135906	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2598	0.2168866003651509	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2596	0.21671963608465425	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2495	0.2082879399195733	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2325	0.19409597607735787	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2296	0.19167499401015645	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2289	0.19109061902841817	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2241	0.18708347629649852	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2163	0.1805718693571291	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2162	0.18048838721688076	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	2066	0.17247410175304145	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2063	0.1722236553322965	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2042	0.17047053038708165	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	2031	0.16955222684435006	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2002	0.1671312447771486	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1944	0.16228928064274567	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1896	0.15828213791082604	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1868	0.15594463798387292	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	1782	0.1487651739225169	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1762	0.14709553111755036	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1743	0.14550937045283216	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	1687	0.14083437059892592	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1676	0.13991606705619433	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	1667	0.13916472779395941	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1663	0.1388307992329661	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1583	0.1321522280131	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1563	0.1304825852081335	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1550	0.12939731738490526	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	1547	0.12914687096416028	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT	1499	0.12513972823224062	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA	1472	0.12288571044553583	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1456	0.12154999620156261	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGG	1421	0.11862812129287122	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	1421	0.11862812129287122	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1412	0.11787678203063628	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1386	0.1157062463841798	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	1383	0.11545579996343483	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1368	0.11420356785970993	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1357	0.11328526431697834	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1348	0.11253392505474341	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1344	0.1121999964937501	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1341	0.11194955007300512	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA	1339	0.11178258579250848	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTC	1331	0.11111472867052187	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC	1307	0.10911115730456206	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1306	0.10902767516431373	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1293	0.1079424073410855	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCG	1270	0.10602231811537399	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1269	0.10593883597512567	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT	1269	0.10593883597512567	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC	1261	0.10527097885313906	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAG	1247	0.10410222888966249	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAG	1246	0.10401874674941416	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1235	0.10310044320668257	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA	1222	0.10201517538345434	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT	1215	0.10143080040171605	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCC	1203	0.10042901471873614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	8.348214024832597E-5	0.0
17	0.0	0.0	0.0	8.348214024832597E-5	0.0
18	0.0	0.0	0.0	8.348214024832597E-5	0.0
19	0.0	0.0	0.0	1.6696428049665194E-4	0.0
20	0.0	0.0	0.0	3.339285609933039E-4	0.0
21	0.0	0.0	0.0	5.843749817382818E-4	0.0
22	0.0	0.0	0.0	6.678571219866078E-4	0.0
23	0.0	0.0	0.0	0.0011687499634765637	0.0
24	0.0	0.0	0.0	0.002003571365959823	0.0
25	0.0	0.0	0.0	0.0025879463476981054	0.0
26	0.0	0.0	0.0	0.003422767750181365	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAT	40	0.004482674	20.000704	1
TAGAAAT	525	0.0	18.896692	4
TGTCCAC	510	0.0	18.824192	10
AAATGTC	550	0.0	18.620392	7
ATGTCCA	510	0.0	18.511229	9
TTAGAAA	505	0.0	18.376884	3
AATGTCC	560	0.0	18.287884	8
GAAATGT	555	0.0	18.164318	6
ATTTAGA	515	0.0	18.020052	1
TTTAGAA	580	0.0	17.930918	2
ACCGTCG	560	0.0	17.716389	8
ATACCGT	570	0.0	17.68631	6
TACCGTC	570	0.0	17.68631	7
TATCCTT	55	0.0013636058	17.456617	5
TAAGGGT	55	0.0013639875	17.455889	4
TTGTGCC	65	2.0985915E-4	17.232815	7
CGGCATC	495	0.0	17.130487	25
AGATACC	590	0.0	16.814854	4
CCGTCGT	590	0.0	16.814854	9
CGTCGTA	585	0.0	16.68435	10
>>END_MODULE