FastQCFastQC Report
Thu 2 Feb 2017
SRR4063058_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063058_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037772
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24170.2329027956044295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18560.17884467879264423No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14620.1408787286610161No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14100.13586799412587736No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13950.1344225899330489No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13880.1337480679763956No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13450.12960457595695393No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13410.12921913483886635No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13030.1255574442170342No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12620.12160667275663634No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12490.12035398912285165No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11860.11428329151297201No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11830.11399421067440632No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11180.1077307925054829No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10830.10435818272221645No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10820.10426182244269455No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10680.10291277852938795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA350.002066966422.85607732
GACTGCG450.00885454817.776097
ATCGTTT2750.017.45373229
GTATTAG2600.017.2457561
CATCGTT2700.017.18355628
GTCCTAC1601.8189894E-1217.0147861
TTACTCT957.709896E-716.8380744
CGGCATC2800.016.57145325
CGTTTAT3000.016.53256231
TCTAGAT3350.016.2403952
CTACTAG600.002427562816.0139161
GTACTAT1101.9987601E-716.0139161
CGTTATT1407.403287E-1016.0015662
CGAGCCG2700.016.00156615
ACGAACG1703.6379788E-1216.00156615
TCGTTTA3100.015.99925430
TCGTTCT3600.015.99925230
AGTACTA703.70135E-415.996945
TCTAAGC703.7027083E-415.996173
GCATCGT3000.015.46519927
Produced by FastQC (version 0.11.5)