##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063058_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1037772
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268851925085663	32.0	32.0	32.0	32.0	32.0
2	30.95763231229981	32.0	32.0	32.0	32.0	32.0
3	31.000381586706908	32.0	32.0	32.0	32.0	32.0
4	31.04961687152862	32.0	32.0	32.0	32.0	32.0
5	31.004995316890415	32.0	32.0	32.0	32.0	32.0
6	34.62725338513662	36.0	36.0	36.0	32.0	36.0
7	34.567759584957	36.0	36.0	36.0	32.0	36.0
8	34.546598867574	36.0	36.0	36.0	32.0	36.0
9	34.66188045158282	36.0	36.0	36.0	32.0	36.0
10	34.4269801073839	36.0	36.0	36.0	32.0	36.0
11	34.637566825853845	36.0	36.0	36.0	32.0	36.0
12	34.494438084665994	36.0	36.0	36.0	32.0	36.0
13	34.56422701710973	36.0	36.0	36.0	32.0	36.0
14	34.472558519597754	36.0	36.0	36.0	32.0	36.0
15	34.43459931468569	36.0	36.0	36.0	32.0	36.0
16	34.442068199951436	36.0	36.0	36.0	32.0	36.0
17	34.369238137085986	36.0	36.0	36.0	32.0	36.0
18	34.390268768091644	36.0	36.0	36.0	32.0	36.0
19	34.382515620001314	36.0	36.0	36.0	32.0	36.0
20	34.35854985488142	36.0	36.0	36.0	32.0	36.0
21	34.33748742498352	36.0	36.0	36.0	32.0	36.0
22	34.31525132688105	36.0	36.0	36.0	32.0	36.0
23	34.26566336343628	36.0	36.0	36.0	32.0	36.0
24	34.25344102558173	36.0	36.0	36.0	32.0	36.0
25	34.22417351788254	36.0	36.0	36.0	32.0	36.0
26	34.1852015664327	36.0	36.0	36.0	32.0	36.0
27	34.189522361366464	36.0	36.0	36.0	32.0	36.0
28	34.15955816884634	36.0	36.0	36.0	32.0	36.0
29	34.133554383814555	36.0	36.0	36.0	32.0	36.0
30	34.10880424601935	36.0	36.0	36.0	32.0	36.0
31	34.124759581102595	36.0	36.0	36.0	32.0	36.0
32	34.07617761897604	36.0	36.0	36.0	32.0	36.0
33	34.046276060637595	36.0	36.0	36.0	32.0	36.0
34	34.05296442763921	36.0	36.0	36.0	32.0	36.0
35	34.00481994118169	36.0	36.0	36.0	32.0	36.0
36	33.96039496151371	36.0	36.0	36.0	32.0	36.0
37	33.96119860624492	36.0	36.0	36.0	32.0	36.0
38	33.343537886934705	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	9.0
11	11.0
12	3.0
13	1.0
14	190.0
15	369.0
16	451.0
17	536.0
18	635.0
19	837.0
20	1085.0
21	1348.0
22	1996.0
23	2801.0
24	4142.0
25	6007.0
26	8726.0
27	12663.0
28	17988.0
29	24496.0
30	33966.0
31	45390.0
32	63712.0
33	97974.0
34	225717.0
35	486711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24749456851009	17.42649640923527	11.34091233594585	25.985096686308783
2	16.558943645948624	19.881067614834926	37.65044908159055	25.909539657625903
3	18.588429621448885	24.058007048504	29.032242520962892	28.321320809084227
4	12.335303952319693	15.319081277192373	36.49624907853085	35.84936569195708
5	14.460939246536796	36.66570948420071	33.833766313858206	15.039584955404292
6	33.950586353430914	35.91942337897607	16.899698391743836	13.230291875849176
7	29.82408467370482	30.761766553732418	21.22595329224531	18.188195480317447
8	28.054027437705308	32.880631431465	19.802961930834318	19.262379199995376
9	27.46868046996881	14.04327158881873	18.82101157191712	39.667036369295346
10	15.94412238573554	26.434135567872584	31.718168222569975	25.903573823821898
11	37.55679348899688	21.095619276685834	22.468877408935285	18.878709825382003
12	24.469933423079034	23.90891036061776	28.92362011164571	22.69753610465749
13	29.419644836286608	19.219144839177876	25.455519725213616	25.9056905993219
14	23.517660289861777	20.07108253418486	25.12279951082573	31.288457665127638
15	25.193465590642326	27.203059594823443	22.419165647412154	25.184309167122077
16	25.768848250287185	25.62756828852722	23.930281326374057	24.673302134811536
17	23.906441405884806	25.92108547707924	25.418364170312262	24.754108946723687
18	25.021730457524903	24.495188462331623	26.50508714347113	23.97799393667234
19	25.56471477085686	24.990266861457926	25.48819881043701	23.956819557248203
20	25.587858615196772	24.19199253739254	25.18697474335241	25.033174104058276
21	27.112243088641165	24.18725041244584	24.31146599441849	24.389040504494503
22	25.636817612814504	24.304735766344578	25.06593915962143	24.99250746121949
23	24.105401321419485	24.00132219044168	25.68740411228211	26.205872375856725
24	24.686757008634242	25.223276886469232	25.321290307658057	24.768675797238473
25	24.84254271706456	24.535579322328914	25.391510847806874	25.23036711279965
26	24.436012324987207	25.481727926967608	25.929602618433318	24.15265712961187
27	25.326834232452715	24.782544170564776	24.815309507151518	25.075312089830987
28	24.245721119423195	24.76465072712783	25.89502107037302	25.094607083075953
29	24.345179641324492	24.8890041063903	25.830457446675233	24.93535880560998
30	24.39413839922832	25.14750847276333	26.067972906430047	24.3903802215783
31	25.035992575989148	24.808280508887805	24.71470944788256	25.44101746724049
32	24.43428100059169	25.077526775406522	24.67510065471338	25.81309156928841
33	24.24177594806917	24.471999290730935	25.48087671824286	25.805348042957032
34	25.158917089417603	24.707897090342776	25.619767011549193	24.513418808690425
35	25.917855377121825	24.587489121624813	25.528595068084414	23.96606043316895
36	24.396609467084943	25.4209975954009	24.973231366766417	25.20916157074774
37	25.526561277315846	25.095309133101278	24.906907358366194	24.47122223121668
38	24.447969286335155	24.907151681484493	25.50683521663965	25.1380438155407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	371.0
1	304.0
2	237.0
3	237.0
4	523.5
5	810.0
6	810.0
7	882.5
8	955.0
9	936.0
10	917.0
11	917.0
12	1140.0
13	1363.0
14	1854.0
15	2345.0
16	2345.0
17	3607.5
18	4870.0
19	4870.0
20	5831.5
21	6793.0
22	7697.5
23	8602.0
24	8602.0
25	10038.0
26	11474.0
27	11474.0
28	14425.0
29	17376.0
30	21090.0
31	24804.0
32	24804.0
33	31209.0
34	37614.0
35	37614.0
36	41590.5
37	45567.0
38	51808.0
39	58049.0
40	58049.0
41	60954.0
42	63859.0
43	72604.0
44	81349.0
45	81349.0
46	82990.0
47	84631.0
48	84631.0
49	89816.0
50	95001.0
51	95803.0
52	96605.0
53	96605.0
54	91617.5
55	86630.0
56	86630.0
57	83823.0
58	81016.0
59	72838.5
60	64661.0
61	64661.0
62	61632.0
63	58603.0
64	48200.0
65	37797.0
66	37797.0
67	31682.0
68	25567.0
69	25567.0
70	20467.0
71	15367.0
72	11997.0
73	8627.0
74	8627.0
75	6434.0
76	4241.0
77	4241.0
78	4254.0
79	4267.0
80	3344.0
81	2421.0
82	2421.0
83	2491.5
84	2562.0
85	2562.0
86	1671.5
87	781.0
88	686.0
89	591.0
90	591.0
91	431.5
92	272.0
93	228.5
94	185.0
95	185.0
96	161.0
97	137.0
98	137.0
99	296.0
100	455.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11794498213480419
2	0.05241999205991296
3	0.010117829349799379
4	0.002601727547091269
5	3.8544111808759535E-4
6	1.9272055904379768E-4
7	0.0
8	2.890808385656965E-4
9	9.636027952189884E-5
10	3.8544111808759535E-4
11	0.0020235658699598756
12	0.002409006988047471
13	0.015995806400635207
14	0.010117829349799379
15	0.02399370960095281
16	0.011177792424540265
17	0.021102901215295847
18	0.005588896212270132
19	0.007708822361751907
20	0.006263418168923424
21	0.005781616771313931
22	0.007034300405098615
23	0.009636027952189884
24	0.015321284443981916
25	0.01821209282963888
26	0.01975385730198926
27	0.008672425156970896
28	0.005685256491792031
29	0.011659593822149759
30	0.0033726097832664594
31	0.005588896212270132
32	0.006359778448445323
33	0.008094263479839502
34	0.011948674660715456
35	0.01570672556206951
36	0.017055769475376095
37	0.009250586834102289
38	0.005588896212270132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1037772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.97782877766233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60867160676875	46.24254074753837
2	10.804044600712427	12.095739175298148
3	2.8609483840236183	4.8044903634781155
4	1.1777099231758146	2.6370257771715844
5	0.616509135594003	1.7255421416072856
6	0.38875562070052405	1.3057017343156667
7	0.2719804633332477	1.0657413065136467
8	0.20046835266203622	0.8977426496524393
9	0.13402752996744577	0.6752313111609618
>10	0.7488462915051064	7.775534558211312
>50	0.07995209042477336	3.157260883556323
>100	0.0942730939530512	11.055353770318044
>500	0.010359680384421895	4.02739506837293
>1k	0.0034532267948072983	2.534700512805205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2417	0.2329027956044295	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1856	0.17884467879264423	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1462	0.1408787286610161	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1410	0.13586799412587736	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1395	0.1344225899330489	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1388	0.1337480679763956	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1345	0.12960457595695393	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1341	0.12921913483886635	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1303	0.1255574442170342	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1262	0.12160667275663634	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1249	0.12035398912285165	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1186	0.11428329151297201	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1183	0.11399421067440632	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1118	0.1077307925054829	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1083	0.10435818272221645	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1082	0.10426182244269455	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1068	0.10291277852938795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.636027952189884E-5
2	0.0	0.0	0.0	0.0	9.636027952189884E-5
3	0.0	0.0	0.0	0.0	9.636027952189884E-5
4	0.0	0.0	0.0	0.0	9.636027952189884E-5
5	0.0	0.0	0.0	0.0	9.636027952189884E-5
6	0.0	0.0	0.0	0.0	9.636027952189884E-5
7	0.0	0.0	0.0	0.0	9.636027952189884E-5
8	0.0	0.0	0.0	0.0	9.636027952189884E-5
9	0.0	0.0	0.0	0.0	9.636027952189884E-5
10	0.0	0.0	0.0	0.0	9.636027952189884E-5
11	0.0	0.0	0.0	0.0	9.636027952189884E-5
12	0.0	0.0	0.0	0.0	3.8544111808759535E-4
13	0.0	0.0	0.0	0.0	5.78161677131393E-4
14	0.0	0.0	0.0	0.0	5.78161677131393E-4
15	0.0	0.0	0.0	9.636027952189884E-5	6.745219566532918E-4
16	0.0	0.0	0.0	9.636027952189884E-5	8.672425156970896E-4
17	0.0	0.0	0.0	1.9272055904379768E-4	8.672425156970896E-4
18	0.0	0.0	0.0	1.9272055904379768E-4	8.672425156970896E-4
19	0.0	0.0	0.0	1.9272055904379768E-4	8.672425156970896E-4
20	0.0	0.0	0.0	1.9272055904379768E-4	0.0010599630747408872
21	0.0	0.0	0.0	3.8544111808759535E-4	0.001156323354262786
22	0.0	0.0	0.0	3.8544111808759535E-4	0.001252683633784685
23	0.0	0.0	0.0	9.636027952189883E-4	0.0014454041928284825
24	0.0	0.0	0.0	0.0017344850313941792	0.0015417644723503814
25	0.0	0.0	0.0	0.002312646708525572	0.0015417644723503814
26	0.0	0.0	0.0	0.002794448106135066	0.0015417644723503814
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	35	0.0020669664	22.856077	32
GACTGCG	45	0.008854548	17.77609	7
ATCGTTT	275	0.0	17.453732	29
GTATTAG	260	0.0	17.245756	1
CATCGTT	270	0.0	17.183556	28
GTCCTAC	160	1.8189894E-12	17.014786	1
TTACTCT	95	7.709896E-7	16.838074	4
CGGCATC	280	0.0	16.571453	25
CGTTTAT	300	0.0	16.532562	31
TCTAGAT	335	0.0	16.240395	2
CTACTAG	60	0.0024275628	16.013916	1
GTACTAT	110	1.9987601E-7	16.013916	1
CGTTATT	140	7.403287E-10	16.001566	2
CGAGCCG	270	0.0	16.001566	15
ACGAACG	170	3.6379788E-12	16.001566	15
TCGTTTA	310	0.0	15.999254	30
TCGTTCT	360	0.0	15.999252	30
AGTACTA	70	3.70135E-4	15.99694	5
TCTAAGC	70	3.7027083E-4	15.99617	3
GCATCGT	300	0.0	15.465199	27
>>END_MODULE