FastQCFastQC Report
Thu 2 Feb 2017
SRR4063058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037772
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27700.2669179742756598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21350.20572919677925403No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15910.15330920471934104No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14590.1405896478224504No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14400.13875880251153433No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13660.13162814182691382No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13530.13037545819312912No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13460.12970093623647586No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12980.1250756428194247No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12930.1245938414218152No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12600.12141395219759253No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12440.11987218772524216No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12220.11775226157576038No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12200.11755954101671659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11850.11418693123345011No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11740.11312696815870923No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11540.11119976256827127No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10650.10262369769082225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAT2650.018.1126352
ACGATTA450.0088537817.7763632
TCTAGCG3000.017.59859528
ATTCGTT550.001365254417.45315432
ATCGTTT3150.016.76056729
CGGGTCG1456.91216E-1116.55040429
CGGCATC3350.016.2375125
TAATACT1501.1459633E-1016.0010364
AGGGGCG2800.015.99872432
CTAGCGG3300.015.99872229
TCGTTTA3250.015.75258930
CGTTTAT3250.015.75258931
AGAACCG2750.015.7123795
TTAGGAC1753.6379788E-1215.5423663
CATCGTT3500.015.54161728
GCATCGT3500.015.54161727
CGGCGTT1652.910383E-1115.51391230
ATACCGT2900.015.4522536
AACCGCG2500.015.3639567
GTCTTAG1255.124457E-815.3624751
Produced by FastQC (version 0.11.5)