FastQCFastQC Report
Thu 2 Feb 2017
SRR4063057_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063057_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634884
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44400.6993403519383068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31390.494421028093321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17790.28020866804014594No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.2173625418186629No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11360.17893032427971092No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11330.17845779701488776No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10770.16963728807152173No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10200.16065927003988129No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC9350.14727099753655787No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC9200.14490836121244197No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9190.14475085212416755No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9160.1442783248593444No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8940.14081312491730774No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG8920.14049810674075897No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8880.13986807038766136No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8650.13624536135735033No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8620.13577283409252713No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8530.1343552522980576No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT8340.13136257962084413No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA8010.12616477970778914No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG7880.12411716156022204No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG7670.12080947070645977No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7340.11561167079340477No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC7300.11498163444030722No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7230.11387907082238645No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG7170.1129340162927401No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA7150.1126189981161913No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG6980.10994134361552663No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT6810.10726368911486193No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6500.10238090737835573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA453.5053559E-421.3593651
CTAGCGG2100.021.3324429
TCTAGCG2150.020.83633628
TAGCGGC2150.020.09218230
GTAAGTT400.004478304620.0023169
GCGGCGC2300.019.47744632
GTCCCCG507.1854837E-419.2022211
ATAGCGT1500.019.1931486
AGCGGCG2300.018.78182231
CCAATAG1900.018.5226213
AATAGCG1600.017.9935765
GATTAGA450.00878409417.799471
CGTGCGC450.008840132517.77983510
AGCGTAT1550.017.5476538
TAGCGTA1550.017.5476537
TAGGACG550.001366999617.4483174
ACGCATC652.0992085E-417.23004720
CGCATCC753.2311662E-517.06595221
GGACGTG753.2399163E-517.0605756
CGTATAT1601.8189894E-1217.00196810
Produced by FastQC (version 0.11.5)