##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063057_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634884
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11560852061164	32.0	32.0	32.0	32.0	32.0
2	30.807588472854885	32.0	32.0	32.0	32.0	32.0
3	30.867589984942132	32.0	32.0	32.0	32.0	32.0
4	30.916517033032807	32.0	32.0	32.0	32.0	32.0
5	30.894898910667145	32.0	32.0	32.0	32.0	32.0
6	34.485211471701916	36.0	36.0	36.0	32.0	36.0
7	34.3682593985673	36.0	36.0	36.0	32.0	36.0
8	34.335395127298845	36.0	36.0	36.0	32.0	36.0
9	34.437519294863314	36.0	36.0	36.0	32.0	36.0
10	34.24951172182635	36.0	36.0	36.0	32.0	36.0
11	34.50082534762256	36.0	36.0	36.0	32.0	36.0
12	34.32149967553128	36.0	36.0	36.0	32.0	36.0
13	34.421308459498114	36.0	36.0	36.0	32.0	36.0
14	34.311916822600665	36.0	36.0	36.0	32.0	36.0
15	34.298328513555234	36.0	36.0	36.0	32.0	36.0
16	34.297783532109804	36.0	36.0	36.0	32.0	36.0
17	34.221489279931454	36.0	36.0	36.0	32.0	36.0
18	34.253786518482116	36.0	36.0	36.0	32.0	36.0
19	34.24031004088936	36.0	36.0	36.0	32.0	36.0
20	34.18088343697431	36.0	36.0	36.0	32.0	36.0
21	34.1600922373221	36.0	36.0	36.0	32.0	36.0
22	34.15191909073153	36.0	36.0	36.0	32.0	36.0
23	34.11796485657222	36.0	36.0	36.0	32.0	36.0
24	34.11000592234172	36.0	36.0	36.0	32.0	36.0
25	34.08086516592007	36.0	36.0	36.0	32.0	36.0
26	34.05024224897777	36.0	36.0	36.0	32.0	36.0
27	34.043784376358516	36.0	36.0	36.0	32.0	36.0
28	34.03768562446053	36.0	36.0	36.0	32.0	36.0
29	33.995466888439466	36.0	36.0	36.0	32.0	36.0
30	33.94544672727616	36.0	36.0	36.0	32.0	36.0
31	33.9788874818077	36.0	36.0	36.0	32.0	36.0
32	33.919473478619715	36.0	36.0	36.0	32.0	36.0
33	33.855356254055856	36.0	36.0	36.0	32.0	36.0
34	33.848512484170335	36.0	36.0	36.0	32.0	36.0
35	33.76660145790412	36.0	36.0	36.0	32.0	36.0
36	33.718832101612264	36.0	36.0	36.0	27.0	36.0
37	33.71351932006477	36.0	36.0	36.0	27.0	36.0
38	33.10760390874553	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	3.0
11	4.0
12	1.0
13	4.0
14	96.0
15	198.0
16	242.0
17	360.0
18	482.0
19	680.0
20	834.0
21	1156.0
22	1570.0
23	2127.0
24	3169.0
25	4427.0
26	6273.0
27	8951.0
28	12355.0
29	17157.0
30	22846.0
31	30615.0
32	41780.0
33	61672.0
34	136020.0
35	281856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39582617142533	18.83064045592312	12.001412932750078	23.772120439901474
2	15.882561520459543	20.890244190719333	38.911503518269	24.315690770552127
3	18.339686840364198	26.273274313978767	29.426136542642006	25.960902303015025
4	11.008430098855195	16.24999212434711	38.4423218684829	34.299255908314805
5	12.785367989642168	38.94897004482723	33.780923069168765	14.484738896361844
6	31.018471119875628	38.1457685904332	18.1916983129175	12.644061976773674
7	28.53592152267186	32.65210652654658	21.37587338789448	17.43609856288708
8	28.17814964040562	32.226618489735095	20.18406570039787	19.41116616946141
9	27.767478417283186	14.204349462814408	20.289565163975094	37.73860695592731
10	16.13829993337334	26.74123182139645	31.684520406186355	25.435947839043855
11	36.0731550188936	22.778800732747282	22.99207240526028	18.155971843098833
12	24.24831422977509	26.207918749104152	29.10172585698242	20.442041164138338
13	28.010529350327744	20.60863551655096	27.0517976612996	24.329037471821703
14	23.096589432296664	21.744712288975947	26.486521397370282	28.67217688135711
15	24.436570567911662	28.103489835819694	24.673679633355338	22.786259962913306
16	24.413726833801974	27.243527641911424	25.304222490016777	23.038523034269826
17	22.034101238741755	26.94889540406801	26.686590301485452	24.33041305570478
18	23.276968756645218	25.489599987398698	28.831622969024423	22.40180828693166
19	23.745900528953843	26.3090741259975	28.17269917679786	21.772326168250796
20	24.159011290891677	25.00094510812037	27.75719542315641	23.082848177831544
21	26.799066855899806	24.446674217089896	26.35076704003982	22.403491886970478
22	24.48404159801398	25.371039540796236	27.282708344491034	22.86221051669875
23	23.027908654565618	25.110385447236304	28.023647610901342	23.838058287296736
24	22.674564229396893	26.953583440323253	27.53282555785726	22.839026772422592
25	23.191960628631204	25.772240916480776	27.66141126948719	23.374387185400835
26	22.25663549517304	26.80947999899176	28.923165629017216	22.010718876817986
27	23.759191466034896	26.113529964021755	27.246592777886292	22.88068579205706
28	22.145896110728692	26.04052302200996	28.742897917457537	23.07068294980381
29	22.17718751329119	26.17375976459432	28.88634398151284	22.762708740601653
30	22.584572831789966	26.50870182258646	28.73814515481195	22.168580190811618
31	22.85999618805791	26.354066412852227	27.247194993439365	23.538742405650495
32	22.258259128634954	26.505293434754734	27.292889738092725	23.943557698517587
33	22.34638455528392	26.045000882123144	27.649834916954408	23.958779645638533
34	23.295646982282637	26.0763609383098	28.590849730860533	22.037142348547025
35	24.255685837118115	25.97297642171527	27.639068085113088	22.132269656053523
36	22.194584035665336	26.853605129255342	27.47333763921927	23.478473195860047
37	23.99034690039098	26.76624313587116	26.765770562456876	22.47763940128099
38	21.977509612522034	26.717376888047394	27.75856936058811	23.546544138842464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	87.0
2	127.0
3	127.0
4	430.5
5	734.0
6	734.0
7	985.0
8	1236.0
9	1347.5
10	1459.0
11	1459.0
12	1904.5
13	2350.0
14	3004.0
15	3658.0
16	3658.0
17	5220.5
18	6783.0
19	6783.0
20	7800.5
21	8818.0
22	9355.0
23	9892.0
24	9892.0
25	10841.5
26	11791.0
27	11791.0
28	15172.5
29	18554.0
30	21366.5
31	24179.0
32	24179.0
33	28180.5
34	32182.0
35	32182.0
36	33257.0
37	34332.0
38	38044.0
39	41756.0
40	41756.0
41	42420.0
42	43084.0
43	48080.0
44	53076.0
45	53076.0
46	51972.0
47	50868.0
48	50868.0
49	51610.5
50	52353.0
51	50453.0
52	48553.0
53	48553.0
54	45311.0
55	42069.0
56	42069.0
57	39863.0
58	37657.0
59	32989.5
60	28322.0
61	28322.0
62	27825.5
63	27329.0
64	23265.5
65	19202.0
66	19202.0
67	16429.5
68	13657.0
69	13657.0
70	11008.5
71	8360.0
72	6304.5
73	4249.0
74	4249.0
75	3271.5
76	2294.0
77	2294.0
78	2153.0
79	2012.0
80	1607.5
81	1203.0
82	1203.0
83	1236.0
84	1269.0
85	1269.0
86	852.5
87	436.0
88	368.5
89	301.0
90	301.0
91	239.5
92	178.0
93	150.0
94	122.0
95	122.0
96	105.5
97	89.0
98	89.0
99	196.0
100	303.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11687174349959992
2	0.05339558092501938
3	0.010080581649561179
4	0.0025201454123902948
5	3.1501817654878685E-4
6	1.5750908827439342E-4
7	0.0
8	3.1501817654878685E-4
9	1.5750908827439342E-4
10	4.7252726482318024E-4
11	0.0020476181475671145
12	0.0017325999710183278
13	0.013703290679872228
14	0.009923072561286786
15	0.02441390868253098
16	0.010868127090933146
17	0.020003654210847967
18	0.004567763559957409
19	0.007245418060622098
20	0.005670327177878164
21	0.00645787261925013
22	0.007245418060622098
23	0.009923072561286786
24	0.014018308856421016
25	0.01701098153363449
26	0.018271054239829637
27	0.008820508943366032
28	0.005512818089603771
29	0.010238090737835572
30	0.0026776545006646883
31	0.005827836266152557
32	0.00645787261925013
33	0.008190472590268458
34	0.013073254326774654
35	0.01653845426881131
36	0.014805854297792983
37	0.009765563473012393
38	0.005512818089603771
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	634884.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.80954130278282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.49317732168329	34.276241885770574
2	13.912133294570475	12.467926229457515
3	4.483324110056786	6.026870906500548
4	1.8104444849175443	3.245007476932322
5	0.8588075247197162	1.9241385625034402
6	0.47842102738157655	1.2862696071944724
7	0.28341111179856476	0.8889665343863771
8	0.2030609175008038	0.7279253255786594
9	0.1392817202245453	0.5617034995611964
>10	0.9588739376045825	8.960802791683566
>50	0.1749257955452738	5.56033559125428
>100	0.1851327320730834	16.74046455542144
>500	0.01619031497217079	4.951247296178818
>1k	0.002815706951681876	2.3820997375767616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4440	0.6993403519383068	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3139	0.494421028093321	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1779	0.28020866804014594	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1380	0.2173625418186629	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1136	0.17893032427971092	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1133	0.17845779701488776	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1077	0.16963728807152173	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1020	0.16065927003988129	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	935	0.14727099753655787	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	920	0.14490836121244197	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	919	0.14475085212416755	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	916	0.1442783248593444	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	894	0.14081312491730774	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	892	0.14049810674075897	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	888	0.13986807038766136	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	865	0.13624536135735033	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	862	0.13577283409252713	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	853	0.1343552522980576	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	834	0.13136257962084413	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	801	0.12616477970778914	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	788	0.12411716156022204	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	767	0.12080947070645977	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	734	0.11561167079340477	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	730	0.11498163444030722	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	723	0.11387907082238645	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	717	0.1129340162927401	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	715	0.1126189981161913	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	698	0.10994134361552663	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	681	0.10726368911486193	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	650	0.10238090737835573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5750908827439342E-4	0.0
3	0.0	0.0	0.0	1.5750908827439342E-4	0.0
4	0.0	0.0	0.0	1.5750908827439342E-4	0.0
5	0.0	0.0	0.0	1.5750908827439342E-4	0.0
6	0.0	0.0	0.0	1.5750908827439342E-4	0.0
7	0.0	0.0	0.0	1.5750908827439342E-4	0.0
8	0.0	0.0	0.0	1.5750908827439342E-4	0.0
9	0.0	0.0	0.0	3.1501817654878685E-4	0.0
10	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
11	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
12	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
13	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
14	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
15	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
16	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
17	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
18	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
19	0.0	0.0	1.5750908827439342E-4	3.1501817654878685E-4	0.0
20	0.0	0.0	1.5750908827439342E-4	4.725272648231803E-4	0.0
21	0.0	0.0	1.5750908827439342E-4	6.300363530975737E-4	0.0
22	0.0	0.0	1.5750908827439342E-4	7.875454413719671E-4	0.0
23	0.0	0.0	1.5750908827439342E-4	0.0025201454123902948	0.0
24	0.0	0.0	1.5750908827439342E-4	0.004252745383408623	0.0
25	0.0	0.0	1.5750908827439342E-4	0.004252745383408623	0.0
26	0.0	0.0	1.5750908827439342E-4	0.00598534535442695	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTATA	45	3.5053559E-4	21.359365	1
CTAGCGG	210	0.0	21.33244	29
TCTAGCG	215	0.0	20.836336	28
TAGCGGC	215	0.0	20.092182	30
GTAAGTT	40	0.0044783046	20.002316	9
GCGGCGC	230	0.0	19.477446	32
GTCCCCG	50	7.1854837E-4	19.20222	11
ATAGCGT	150	0.0	19.193148	6
AGCGGCG	230	0.0	18.781822	31
CCAATAG	190	0.0	18.522621	3
AATAGCG	160	0.0	17.993576	5
GATTAGA	45	0.008784094	17.79947	1
CGTGCGC	45	0.0088401325	17.779835	10
AGCGTAT	155	0.0	17.547653	8
TAGCGTA	155	0.0	17.547653	7
TAGGACG	55	0.0013669996	17.448317	4
ACGCATC	65	2.0992085E-4	17.230047	20
CGCATCC	75	3.2311662E-5	17.065952	21
GGACGTG	75	3.2399163E-5	17.060575	6
CGTATAT	160	1.8189894E-12	17.001968	10
>>END_MODULE