FastQCFastQC Report
Thu 2 Feb 2017
SRR4063057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634884
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38890.612552844299116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29140.4589814832315825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17780.28005115895187155No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA13170.20743946925737616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.20066657846157723No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11490.18097794242727805No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10890.17152739713081444No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10260.16160432456952767No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9880.1556189792151007No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9850.15514645195027751No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9580.1508937065668689No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT9210.14506587030071635No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9060.14270323397660045No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC8980.1414431612704053No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8850.1393955431228382No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8750.13782045224009423No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8530.1343552522980576No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8510.1340402341215088No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8460.13325268868013684No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8050.12679481606088672No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA7830.12332961611885006No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7790.12269957976575249No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7790.12269957976575249No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG7740.12191203432438051No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC7550.11891936164716704No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG7500.11813181620579508No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC7170.1129340162927401No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT6850.1078937254679595No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA6690.10537358005556921No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA6680.10521607096729481No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC6670.1050585618790204No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG6630.10442852552592286No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6600.10395599826109966No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC6390.1006483074073374No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT6350.10001827105423983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTAA651.4013494E-824.61623210
GTATAGA851.0510121E-820.7114891
AAGACGG2650.020.5306265
TCTAGCG1800.020.44353928
CGAACTC555.8300117E-520.36273420
TAGAAGA801.262797E-720.000694
CCCGTTG801.263852E-719.99911531
TATAAGG400.004482455619.9991152
TAACCCG902.0350853E-819.5546928
CAAGACG2750.019.200664
AGCGGCG2050.018.73087731
CGCAAGA2750.018.6173572
GGAGTGT953.7765858E-818.5284146
CTAGACA953.77986E-818.5269554
CTAGCGG1900.018.52549629
TAGCGGC2000.018.39918330
CAAGTAG701.7858902E-518.28490430
GCGCAAG2850.017.9697761
ACCGTCG1700.017.8843788
CTTAGAC450.00884427917.778393
Produced by FastQC (version 0.11.5)