##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634884
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.050172944978925	32.0	32.0	32.0	32.0	32.0
2	31.24928175855747	32.0	32.0	32.0	32.0	32.0
3	31.352402958650714	32.0	32.0	32.0	32.0	32.0
4	31.446732001436484	32.0	32.0	32.0	32.0	32.0
5	31.36840430692851	32.0	32.0	32.0	32.0	32.0
6	34.8432312044405	36.0	36.0	36.0	32.0	36.0
7	34.878187196401235	36.0	36.0	36.0	32.0	36.0
8	34.832046484082134	36.0	36.0	36.0	32.0	36.0
9	34.945493980002645	36.0	36.0	36.0	32.0	36.0
10	34.78351793398479	36.0	36.0	36.0	32.0	36.0
11	34.96671832964762	36.0	36.0	36.0	36.0	36.0
12	34.85905299235766	36.0	36.0	36.0	32.0	36.0
13	34.923389154554215	36.0	36.0	36.0	32.0	36.0
14	34.846693254200765	36.0	36.0	36.0	32.0	36.0
15	34.844625159871725	36.0	36.0	36.0	32.0	36.0
16	34.83525966948293	36.0	36.0	36.0	32.0	36.0
17	34.80769085376226	36.0	36.0	36.0	32.0	36.0
18	34.80066909860699	36.0	36.0	36.0	32.0	36.0
19	34.780331525129	36.0	36.0	36.0	32.0	36.0
20	34.76322761323328	36.0	36.0	36.0	32.0	36.0
21	34.749300659648064	36.0	36.0	36.0	32.0	36.0
22	34.74070853888269	36.0	36.0	36.0	32.0	36.0
23	34.685170834357145	36.0	36.0	36.0	32.0	36.0
24	34.65671681756037	36.0	36.0	36.0	32.0	36.0
25	34.62469521991419	36.0	36.0	36.0	32.0	36.0
26	34.572358100062374	36.0	36.0	36.0	32.0	36.0
27	34.5672626810567	36.0	36.0	36.0	32.0	36.0
28	34.529772367865625	36.0	36.0	36.0	32.0	36.0
29	34.48687634276498	36.0	36.0	36.0	32.0	36.0
30	34.459917087215935	36.0	36.0	36.0	32.0	36.0
31	34.448817421765234	36.0	36.0	36.0	32.0	36.0
32	34.40668846592448	36.0	36.0	36.0	32.0	36.0
33	34.35958537307603	36.0	36.0	36.0	32.0	36.0
34	34.33306556788327	36.0	36.0	36.0	32.0	36.0
35	34.28328797071591	36.0	36.0	36.0	32.0	36.0
36	34.25093717907523	36.0	36.0	36.0	32.0	36.0
37	34.24769249185678	36.0	36.0	36.0	32.0	36.0
38	33.809250193736176	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	9.0
22	41.0
23	137.0
24	376.0
25	987.0
26	2274.0
27	4491.0
28	7892.0
29	12630.0
30	19275.0
31	27999.0
32	40103.0
33	62776.0
34	147739.0
35	308150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56824661720461	20.286218357670556	12.766094860041271	24.379440165083565
2	14.945879877268919	22.020243068025025	38.01592101864277	25.017956036063282
3	17.30237334694212	26.935944203980572	29.90514802704116	25.856534422036155
4	10.66887809426604	17.121395404514843	38.27581101429553	33.933915486923595
5	12.301459794230126	39.499656630187566	33.52061163929159	14.678271936290724
6	31.03658469355771	38.64995597614049	17.716177626428806	12.597281703872998
7	28.311245239272807	32.93761675397948	21.16755554575496	17.58358246099275
8	28.096347435455186	32.75573386005617	19.84929174365105	19.298626960837588
9	27.744665032731213	13.947781271067372	19.957061940434862	38.350491755766555
10	15.888961868444637	27.164460741461788	31.59552735079487	25.351050039298705
11	36.23289537728226	22.67631524644676	22.809103983189544	18.28168539308144
12	23.87896314427428	26.555370218985917	28.80791833430202	20.75774830243778
13	28.088999215602268	20.604458781316843	26.88310583699018	24.423436166090706
14	22.9744331247913	21.810440962443533	26.290314451143832	28.924811461621335
15	24.446040536538955	28.405188979404112	24.247579085313223	22.90119139874371
16	24.611267570138796	27.324834142930047	24.86627478405504	23.197623502876116
17	22.141052538731483	27.314438543103936	26.22069543412655	24.32381348403803
18	23.331663736997623	25.535688409221212	28.58506435821347	22.547583495567693
19	23.864674491718173	26.46593708456978	27.76412699012733	21.90526143358472
20	24.399732864586287	25.18003288789763	27.106684055670012	23.31355019184607
21	26.858292223461294	24.736959822581763	25.813219422760692	22.59152853119625
22	24.516958242699836	25.695915625398698	26.838173332724296	22.94895279917717
23	23.078970704746244	25.276075359004285	27.603283134949695	24.041670801299773
24	22.669495529892075	27.158347036624015	27.195361672368495	22.976795761115415
25	23.227392720559976	25.946314602352555	27.363108851380723	23.463183825706743
26	22.436382079245973	27.0772298561627	28.255712854631714	22.230675209959614
27	23.822147037884086	26.42403966708879	26.807101769772117	22.946711525255008
28	22.326440735630445	26.168402416819454	28.53529148631876	22.969865361231342
29	22.391964516352594	26.291574523850024	28.366599252776886	22.949861707020496
30	22.61736002167325	26.637937008965416	28.406606561198583	22.338096408162748
31	23.020425778567425	26.569263046477783	26.740790443608596	23.6695207313462
32	22.3777886984079	26.723149425721864	26.912475349827687	23.98658652604255
33	22.3377813899862	26.113746763188235	27.40705388700928	24.14141795981628
34	23.14249532198008	26.322603814240082	28.17869091046553	22.356209953314305
35	24.301762211679616	26.114376799541333	27.3171161976046	22.26674479117445
36	22.278557972795028	27.083372710605403	27.17472798180455	23.463341334795018
37	24.29546184814864	26.866167677875012	26.284329105789407	22.554041368186944
38	22.184525047095217	26.677944317387116	27.433672922927656	23.703857712590015
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	54.0
2	68.0
3	68.0
4	256.5
5	445.0
6	445.0
7	693.0
8	941.0
9	1190.0
10	1439.0
11	1439.0
12	1777.5
13	2116.0
14	2754.0
15	3392.0
16	3392.0
17	4870.5
18	6349.0
19	6349.0
20	7281.5
21	8214.0
22	9034.5
23	9855.0
24	9855.0
25	10923.5
26	11992.0
27	11992.0
28	15519.0
29	19046.0
30	21846.5
31	24647.0
32	24647.0
33	28582.0
34	32517.0
35	32517.0
36	33347.0
37	34177.0
38	37602.5
39	41028.0
40	41028.0
41	41945.5
42	42863.0
43	48054.5
44	53246.0
45	53246.0
46	52325.5
47	51405.0
48	51405.0
49	52311.0
50	53217.0
51	50805.5
52	48394.0
53	48394.0
54	45303.0
55	42212.0
56	42212.0
57	40248.0
58	38284.0
59	33519.0
60	28754.0
61	28754.0
62	28204.5
63	27655.0
64	23311.0
65	18967.0
66	18967.0
67	16512.0
68	14057.0
69	14057.0
70	11242.5
71	8428.0
72	6385.5
73	4343.0
74	4343.0
75	3228.0
76	2113.0
77	2113.0
78	2033.0
79	1953.0
80	1549.5
81	1146.0
82	1146.0
83	1088.0
84	1030.0
85	1030.0
86	668.5
87	307.0
88	246.0
89	185.0
90	185.0
91	108.0
92	31.0
93	23.5
94	16.0
95	16.0
96	10.5
97	5.0
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008505490766817244
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5750908827439342E-4
7	3.1501817654878685E-4
8	0.0023626363241159016
9	0.0025201454123902948
10	4.7252726482318024E-4
11	0.00645787261925013
12	4.7252726482318024E-4
13	3.1501817654878685E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.5750908827439342E-4
23	4.7252726482318024E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	634884.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.54806960369578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67804736017781	31.77406756948935
2	14.766801254922047	12.565977752367308
3	4.911251165485524	6.268927692909304
4	1.990462415813653	3.38761333646319
5	0.9618424270337383	2.0462269266609585
6	0.5436609347933	1.3879033976637387
7	0.32791391468009723	0.9766472846080395
8	0.22123442095267867	0.7530478033142323
9	0.15435143436208668	0.5910620015401509
>10	1.035253692268969	9.223427589696886
>50	0.18862426300829885	5.7905955369050846
>100	0.1982782602894657	17.34129562878539
>500	0.01930799538399292	5.612547314361731
>1k	0.0029704608283066024	2.2806601652346434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3889	0.612552844299116	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2914	0.4589814832315825	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1778	0.28005115895187155	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1317	0.20743946925737616	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1274	0.20066657846157723	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1149	0.18097794242727805	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1089	0.17152739713081444	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1026	0.16160432456952767	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	988	0.1556189792151007	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	985	0.15514645195027751	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	958	0.1508937065668689	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	921	0.14506587030071635	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	906	0.14270323397660045	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	898	0.1414431612704053	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	885	0.1393955431228382	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	875	0.13782045224009423	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	853	0.1343552522980576	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	851	0.1340402341215088	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	846	0.13325268868013684	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	805	0.12679481606088672	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	783	0.12332961611885006	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	779	0.12269957976575249	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	779	0.12269957976575249	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	774	0.12191203432438051	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	755	0.11891936164716704	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	750	0.11813181620579508	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	717	0.1129340162927401	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	685	0.1078937254679595	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	669	0.10537358005556921	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	668	0.10521607096729481	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	667	0.1050585618790204	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	663	0.10442852552592286	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	660	0.10395599826109966	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	639	0.1006483074073374	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	635	0.10001827105423983	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	1.5750908827439342E-4	0.0
20	0.0	0.0	0.0	3.1501817654878685E-4	0.0
21	0.0	0.0	0.0	4.725272648231803E-4	0.0
22	0.0	0.0	0.0	6.300363530975737E-4	0.0
23	0.0	0.0	0.0	0.0025201454123902948	0.0
24	0.0	0.0	0.0	0.004410254471683016	0.0
25	0.0	0.0	0.0	0.004410254471683016	0.0
26	0.0	0.0	0.0	0.00598534535442695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTAA	65	1.4013494E-8	24.616232	10
GTATAGA	85	1.0510121E-8	20.711489	1
AAGACGG	265	0.0	20.530626	5
TCTAGCG	180	0.0	20.443539	28
CGAACTC	55	5.8300117E-5	20.362734	20
TAGAAGA	80	1.262797E-7	20.00069	4
CCCGTTG	80	1.263852E-7	19.999115	31
TATAAGG	40	0.0044824556	19.999115	2
TAACCCG	90	2.0350853E-8	19.55469	28
CAAGACG	275	0.0	19.20066	4
AGCGGCG	205	0.0	18.730877	31
CGCAAGA	275	0.0	18.617357	2
GGAGTGT	95	3.7765858E-8	18.528414	6
CTAGACA	95	3.77986E-8	18.526955	4
CTAGCGG	190	0.0	18.525496	29
TAGCGGC	200	0.0	18.399183	30
CAAGTAG	70	1.7858902E-5	18.284904	30
GCGCAAG	285	0.0	17.969776	1
ACCGTCG	170	0.0	17.884378	8
CTTAGAC	45	0.008844279	17.77839	3
>>END_MODULE