FastQCFastQC Report
Thu 2 Feb 2017
SRR4063056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101060
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23290.21152344104771767No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22700.20616496830327138No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21810.19808184840063212No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18910.17174359253809965No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18040.1638421157793399No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17850.16211650591248433No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17770.1613899333369662No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17320.15730296259967666No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17100.15530488801700182No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17030.1546691370134234No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16110.14631355239496485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14950.13577825004995187No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14670.13323524603563838No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14440.13114634988102375No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14310.12996566944580676No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14190.12887581058252956No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13650.12397144569778212No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13270.12052022596407098No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13120.11915790238497448No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12920.11734147094617914No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12590.11434435907216682No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12550.11398107278440775No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12220.11098396091039545No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT12130.11016656676293754No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11990.1088950647557808No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT11690.10617041759758779No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11300.10262837629193686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGG308.424943E-426.6621653
CATCGTT3000.021.33166728
AAGACGG4800.020.6622375
CGTCCCG555.8283524E-520.36574610
CAAGACG4950.020.037024
CGCAAGA5200.019.3901462
ATCGTTT3150.019.30008129
CTAGCGG4500.019.19850229
GACGGAC5300.019.0156567
GGACCGT701.7904486E-518.2817966
CGGACCA5450.017.90939
GCGCAAG5650.017.8587821
TAGCGGC4850.017.81304230
GCATCGT3700.017.72754127
ACGGACC5700.017.6812238
CGGCATC3350.017.67106425
AGACGGA5800.017.6513886
TCTAGCG4900.017.63127728
GATATAC1006.73499E-817.617831
TGTAGGA2850.017.4084762
Produced by FastQC (version 0.11.5)