##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063056_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1101060
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224615370642834	32.0	32.0	32.0	32.0	32.0
2	30.808723411984815	32.0	32.0	32.0	32.0	32.0
3	30.86054438450221	32.0	32.0	32.0	32.0	32.0
4	30.934365974606287	32.0	32.0	32.0	32.0	32.0
5	30.830257206691734	32.0	32.0	32.0	32.0	32.0
6	34.4832334296042	36.0	36.0	36.0	32.0	36.0
7	34.407546364412475	36.0	36.0	36.0	32.0	36.0
8	34.38711514358891	36.0	36.0	36.0	32.0	36.0
9	34.54837792672516	36.0	36.0	36.0	32.0	36.0
10	34.23774453708245	36.0	36.0	36.0	32.0	36.0
11	34.52570795415327	36.0	36.0	36.0	32.0	36.0
12	34.32751802808203	36.0	36.0	36.0	32.0	36.0
13	34.43715328864912	36.0	36.0	36.0	32.0	36.0
14	34.31852396781284	36.0	36.0	36.0	32.0	36.0
15	34.24267524022306	36.0	36.0	36.0	32.0	36.0
16	34.27448640401068	36.0	36.0	36.0	32.0	36.0
17	34.18386463952918	36.0	36.0	36.0	32.0	36.0
18	34.218508528145605	36.0	36.0	36.0	32.0	36.0
19	34.21202114326195	36.0	36.0	36.0	32.0	36.0
20	34.19636350425953	36.0	36.0	36.0	32.0	36.0
21	34.173517337838085	36.0	36.0	36.0	32.0	36.0
22	34.138938840753454	36.0	36.0	36.0	32.0	36.0
23	34.08645759540806	36.0	36.0	36.0	32.0	36.0
24	34.064034657511854	36.0	36.0	36.0	32.0	36.0
25	34.04154541986813	36.0	36.0	36.0	32.0	36.0
26	33.97496775834196	36.0	36.0	36.0	32.0	36.0
27	33.98639311209198	36.0	36.0	36.0	32.0	36.0
28	33.95904673678092	36.0	36.0	36.0	32.0	36.0
29	33.93505894320019	36.0	36.0	36.0	32.0	36.0
30	33.907611755944274	36.0	36.0	36.0	32.0	36.0
31	33.925367373258496	36.0	36.0	36.0	32.0	36.0
32	33.867181624979565	36.0	36.0	36.0	32.0	36.0
33	33.83463026538063	36.0	36.0	36.0	32.0	36.0
34	33.83546400741104	36.0	36.0	36.0	32.0	36.0
35	33.783982707572704	36.0	36.0	36.0	32.0	36.0
36	33.75241676202932	36.0	36.0	36.0	32.0	36.0
37	33.747829364430636	36.0	36.0	36.0	32.0	36.0
38	33.12454907089532	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	0.0
9	4.0
10	10.0
11	10.0
12	4.0
13	6.0
14	86.0
15	199.0
16	272.0
17	384.0
18	474.0
19	593.0
20	899.0
21	1424.0
22	2162.0
23	3394.0
24	5018.0
25	7572.0
26	11139.0
27	16344.0
28	22746.0
29	31023.0
30	41467.0
31	55741.0
32	75489.0
33	112153.0
34	241204.0
35	471238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.951354791780325	17.065739225313695	11.085561011092926	25.89734497181306
2	17.064340897897466	19.19031842410871	36.96543878046165	26.77990189753217
3	19.031074608672117	23.201908203148065	28.25572174822945	29.51129543995037
4	12.392452487454872	14.479144433595966	36.03469494334824	37.09370813560092
5	14.724228377121602	36.32767089049058	33.492756045105786	15.455344687282027
6	35.196120814926765	35.29983942687747	16.219338525924204	13.284701232271564
7	30.927106606361143	30.044775034966303	20.51059887744537	18.517519481227183
8	28.903941132769145	32.37225422186378	19.11450301256796	19.609301632799117
9	27.307990972294753	13.804578336232067	18.470921071154482	40.4165096203187
10	16.090022750907085	25.746397773044944	30.910899092234267	27.252680383813704
11	38.77867420867494	20.8022829470143	21.340323132912456	19.078719711398296
12	24.786042223907508	23.563556108570573	28.15232939915625	23.498072268365675
13	29.451630484149334	18.491688618403124	25.44817876283041	26.60850213461713
14	23.806751297068697	19.462570303303348	24.274894999200686	32.455783400427265
15	25.563898162658127	26.84118007767255	21.91501442165747	25.679907338011855
16	26.621964578651635	25.24402362392113	23.29392379813937	24.840087999287867
17	24.452967515531842	25.662084859870475	24.8089803534329	25.075967271164778
18	25.815918088935597	23.91664297606083	26.121549610853418	24.145889324150158
19	25.988254122229066	24.661620262638262	24.97452274424105	24.375602870891623
20	25.948824425927842	23.484051684205365	24.751451869855785	25.81567202001101
21	27.526060565326027	23.76372965132385	23.715591288954606	24.994618494395514
22	26.011849453985953	23.847412166807906	24.858966428969126	25.281771950237015
23	24.324896316063878	23.495878983634018	25.433492409227732	26.74573229107437
24	24.91125346084252	24.77745238389373	24.912070982697603	25.39922317256615
25	25.14313952231414	24.046713036937856	24.89869182785287	25.91145561289514
26	24.80880353115056	25.19251365559291	25.482930622411615	24.515752190844918
27	25.841373992136003	24.207355897134065	24.53588527176564	25.415384838964293
28	24.58065395095368	24.134695731153496	25.70145322434151	25.58319709355132
29	24.390175224423693	24.648592872759508	25.6557404596655	25.305491443151297
30	24.571443622379935	24.734110671721364	25.898029285518366	24.79641642038034
31	25.21634877384196	24.66739327883742	24.266212534059946	25.85004541326067
32	25.00059038204076	24.444904639676217	24.239451689491744	26.31505328879128
33	24.474440243094946	23.914388143535376	25.25602446572065	26.355147147649028
34	25.484772846180675	24.330928089757005	25.36659981397512	24.8176992500872
35	26.476957483027185	23.96214657754992	25.19089308715407	24.370002852268826
36	24.614446909125924	25.078709330484717	24.714638936274962	25.592204824114397
37	26.124046850234567	24.603639567466427	24.751692848481547	24.52062073381746
38	24.895026771238744	24.232171808228014	25.12472808686688	25.748073333666365
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	63.5
2	82.0
3	82.0
4	271.0
5	460.0
6	460.0
7	556.5
8	653.0
9	670.0
10	687.0
11	687.0
12	907.0
13	1127.0
14	1571.5
15	2016.0
16	2016.0
17	2862.5
18	3709.0
19	3709.0
20	4684.0
21	5659.0
22	6386.5
23	7114.0
24	7114.0
25	8631.0
26	10148.0
27	10148.0
28	12947.0
29	15746.0
30	19189.5
31	22633.0
32	22633.0
33	29641.0
34	36649.0
35	36649.0
36	40981.0
37	45313.0
38	52329.0
39	59345.0
40	59345.0
41	62410.0
42	65475.0
43	74700.0
44	83925.0
45	83925.0
46	86533.5
47	89142.0
48	89142.0
49	94762.5
50	100383.0
51	102387.5
52	104392.0
53	104392.0
54	98456.5
55	92521.0
56	92521.0
57	90388.5
58	88256.0
59	79793.5
60	71331.0
61	71331.0
62	69554.5
63	67778.0
64	56592.5
65	45407.0
66	45407.0
67	38283.0
68	31159.0
69	31159.0
70	25043.0
71	18927.0
72	14910.0
73	10893.0
74	10893.0
75	8252.0
76	5611.0
77	5611.0
78	5434.0
79	5257.0
80	4122.0
81	2987.0
82	2987.0
83	3015.0
84	3043.0
85	3043.0
86	2038.0
87	1033.0
88	874.5
89	716.0
90	716.0
91	550.0
92	384.0
93	321.0
94	258.0
95	258.0
96	223.0
97	188.0
98	188.0
99	398.0
100	608.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11443518064410658
2	0.052494868581185405
3	0.01171598278022996
4	0.0031787550178918496
5	3.6328628775906857E-4
6	3.6328628775906857E-4
7	0.0
8	5.449294316386028E-4
9	4.5410785969883565E-4
10	4.5410785969883565E-4
11	0.002452182442373713
12	0.0022705392984941784
13	0.01453145151036274
14	0.010535302345012989
15	0.025884148002833632
16	0.012896663215446934
17	0.023341143988520153
18	0.005540115888325795
19	0.007084082611301836
20	0.005994223748024631
21	0.006085045319964398
22	0.007356547327121138
23	0.010716945488892522
24	0.015621310373639948
25	0.019163351679290865
26	0.020798139974206674
27	0.008446406190398343
28	0.005449294316386028
29	0.01171598278022996
30	0.0034512197337111515
31	0.005449294316386028
32	0.007084082611301836
33	0.00826476304651881
34	0.011988447496049262
35	0.01652952609303762
36	0.01580295351751948
37	0.009536265053675549
38	0.005903402176084863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1101060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.67069273118795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21532807738359	39.34680220531966
2	12.358358563317463	12.27696461720774
3	3.6800110378839324	5.483660925303386
4	1.5496209345894727	3.0788298116723998
5	0.765642848287842	1.9015005329568477
6	0.49052756227941857	1.4618906293295797
7	0.3117356723748705	1.0838888757117728
8	0.23265250617210023	0.9244808917772157
9	0.1737044954014729	0.7765220355401348
>10	0.9920166700463584	9.086855270407392
>50	0.10183781807170088	3.558957514901553
>100	0.10805220890686305	11.647724301392849
>500	0.014468007299264432	4.98615089278167
>1k	0.0060435979857686875	4.385771495697785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2329	0.21152344104771767	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2270	0.20616496830327138	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2181	0.19808184840063212	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1891	0.17174359253809965	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1804	0.1638421157793399	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1785	0.16211650591248433	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1777	0.1613899333369662	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1732	0.15730296259967666	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1710	0.15530488801700182	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1703	0.1546691370134234	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1611	0.14631355239496485	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1495	0.13577825004995187	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1467	0.13323524603563838	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1444	0.13114634988102375	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1431	0.12996566944580676	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1419	0.12887581058252956	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1365	0.12397144569778212	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1327	0.12052022596407098	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1312	0.11915790238497448	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1292	0.11734147094617914	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1259	0.11434435907216682	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1255	0.11398107278440775	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1222	0.11098396091039545	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1213	0.11016656676293754	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1199	0.1088950647557808	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1169	0.10617041759758779	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1130	0.10262837629193686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.082157193976713E-5
2	0.0	0.0	0.0	0.0	9.082157193976713E-5
3	0.0	0.0	0.0	0.0	9.082157193976713E-5
4	0.0	0.0	0.0	0.0	9.082157193976713E-5
5	0.0	0.0	0.0	0.0	9.082157193976713E-5
6	0.0	0.0	0.0	0.0	9.082157193976713E-5
7	0.0	0.0	0.0	9.082157193976713E-5	9.082157193976713E-5
8	0.0	0.0	0.0	9.082157193976713E-5	9.082157193976713E-5
9	0.0	0.0	0.0	9.082157193976713E-5	9.082157193976713E-5
10	0.0	0.0	0.0	9.082157193976713E-5	9.082157193976713E-5
11	0.0	0.0	0.0	9.082157193976713E-5	1.8164314387953426E-4
12	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	1.8164314387953426E-4
13	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	2.724647158193014E-4
14	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	2.724647158193014E-4
15	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	2.724647158193014E-4
16	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	2.724647158193014E-4
17	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	2.724647158193014E-4
18	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	2.724647158193014E-4
19	0.0	9.082157193976713E-5	0.0	9.082157193976713E-5	2.724647158193014E-4
20	0.0	1.8164314387953426E-4	0.0	1.8164314387953426E-4	2.724647158193014E-4
21	0.0	1.8164314387953426E-4	0.0	3.632862877590685E-4	2.724647158193014E-4
22	0.0	1.8164314387953426E-4	0.0	7.26572575518137E-4	4.5410785969883565E-4
23	0.0	1.8164314387953426E-4	0.0	0.0010898588632772056	4.5410785969883565E-4
24	0.0	1.8164314387953426E-4	0.0	0.001998074582674877	4.5410785969883565E-4
25	0.0	1.8164314387953426E-4	0.0	0.00254300401431348	4.5410785969883565E-4
26	0.0	1.8164314387953426E-4	0.0	0.003269576589831617	6.3575100357837E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAGG	30	8.424943E-4	26.662165	3
CATCGTT	300	0.0	21.331667	28
AAGACGG	480	0.0	20.662237	5
CGTCCCG	55	5.8283524E-5	20.365746	10
CAAGACG	495	0.0	20.03702	4
CGCAAGA	520	0.0	19.390146	2
ATCGTTT	315	0.0	19.300081	29
CTAGCGG	450	0.0	19.198502	29
GACGGAC	530	0.0	19.015656	7
GGACCGT	70	1.7904486E-5	18.281796	6
CGGACCA	545	0.0	17.9093	9
GCGCAAG	565	0.0	17.858782	1
TAGCGGC	485	0.0	17.813042	30
GCATCGT	370	0.0	17.727541	27
ACGGACC	570	0.0	17.681223	8
CGGCATC	335	0.0	17.671064	25
AGACGGA	580	0.0	17.651388	6
TCTAGCG	490	0.0	17.631277	28
GATATAC	100	6.73499E-8	17.61783	1
TGTAGGA	285	0.0	17.408476	2
>>END_MODULE